<i>Aspergillus flavus</i> NRRL 3251 Growth, Oxidative Status, and Aflatoxins Production Ability <i>In Vitro</i> under Different Illumination Regimes
<i>Aspergillus flavus</i> is the most important mycotoxin-producing fungus involved in the global episodes of aflatoxin B<sub>1</sub> contamination of crops at both the pre-harvest and post-harvest stages. However, in order to effectively control aflatoxin contamination in cr...
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MDPI AG
2018-12-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/10/12/528 |
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| author | Tihomir Kovač Bojan Šarkanj Biljana Crevar Marija Kovač Ante Lončarić Ivica Strelec Chibundu N. Ezekiel Michael Sulyok Rudolf Krska |
| author_facet | Tihomir Kovač Bojan Šarkanj Biljana Crevar Marija Kovač Ante Lončarić Ivica Strelec Chibundu N. Ezekiel Michael Sulyok Rudolf Krska |
| author_sort | Tihomir Kovač |
| collection | DOAJ |
| description | <i>Aspergillus flavus</i> is the most important mycotoxin-producing fungus involved in the global episodes of aflatoxin B<sub>1</sub> contamination of crops at both the pre-harvest and post-harvest stages. However, in order to effectively control aflatoxin contamination in crops using antiaflatoxigenic and/or antifungal compounds, some of which are photosensitive, a proper understanding of the photo-sensitive physiology of potential experimental strains need to be documented. The purpose of the study is therefore to evaluate the effect of visible (VIS) light illumination on growth and conidiation, aflatoxin production ability and modulation of <i>A. flavus</i> oxidative status during in vitro experiment. Aflatoxigenic <i>A. flavus</i> strain was inoculated in aflatoxin-inducing YES media and incubated under three different VIS illumination regimes during a 168 h growth period at 29 °C. VIS illumination reduced <i>A. flavus</i> mycelia biomass yield, both during growth on plates and in liquid media, promoted conidiation and increased the aflatoxin production. Furthermore, aflatoxin production increased with increased reactive oxidative species (ROS) levels at 96 h of growth, confirming illumination-driven oxidative stress modulation activity on <i>A. flavus</i> cells. |
| first_indexed | 2024-04-14T06:45:41Z |
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| id | doaj.art-6e8c08c67f4b4dfa9287a8d9322ff827 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-14T06:45:41Z |
| publishDate | 2018-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-6e8c08c67f4b4dfa9287a8d9322ff8272022-12-22T02:07:11ZengMDPI AGToxins2072-66512018-12-01101252810.3390/toxins10120528toxins10120528<i>Aspergillus flavus</i> NRRL 3251 Growth, Oxidative Status, and Aflatoxins Production Ability <i>In Vitro</i> under Different Illumination RegimesTihomir Kovač0Bojan Šarkanj1Biljana Crevar2Marija Kovač3Ante Lončarić4Ivica Strelec5Chibundu N. Ezekiel6Michael Sulyok7Rudolf Krska8Department of Applied Chemistry and Ecology, Faculty of Food Technology, Josip Juraj Strossmayer University of Osijek, Franje Kuhača 20, Osijek 31000, CroatiaDepartment of Applied Chemistry and Ecology, Faculty of Food Technology, Josip Juraj Strossmayer University of Osijek, Franje Kuhača 20, Osijek 31000, CroatiaDepartment of Applied Chemistry and Ecology, Faculty of Food Technology, Josip Juraj Strossmayer University of Osijek, Franje Kuhača 20, Osijek 31000, CroatiaInspecto Ltd., Industrijska zona Nemetin, Vukovarska cesta 239b, Osijek 31000, CroatiaDepartment of Applied Chemistry and Ecology, Faculty of Food Technology, Josip Juraj Strossmayer University of Osijek, Franje Kuhača 20, Osijek 31000, CroatiaDepartment of Applied Chemistry and Ecology, Faculty of Food Technology, Josip Juraj Strossmayer University of Osijek, Franje Kuhača 20, Osijek 31000, CroatiaCenter for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences Vienna (BOKU), Konrad Lorenzstr. 20, Tulln 3430, AustriaCenter for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences Vienna (BOKU), Konrad Lorenzstr. 20, Tulln 3430, AustriaCenter for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences Vienna (BOKU), Konrad Lorenzstr. 20, Tulln 3430, Austria<i>Aspergillus flavus</i> is the most important mycotoxin-producing fungus involved in the global episodes of aflatoxin B<sub>1</sub> contamination of crops at both the pre-harvest and post-harvest stages. However, in order to effectively control aflatoxin contamination in crops using antiaflatoxigenic and/or antifungal compounds, some of which are photosensitive, a proper understanding of the photo-sensitive physiology of potential experimental strains need to be documented. The purpose of the study is therefore to evaluate the effect of visible (VIS) light illumination on growth and conidiation, aflatoxin production ability and modulation of <i>A. flavus</i> oxidative status during in vitro experiment. Aflatoxigenic <i>A. flavus</i> strain was inoculated in aflatoxin-inducing YES media and incubated under three different VIS illumination regimes during a 168 h growth period at 29 °C. VIS illumination reduced <i>A. flavus</i> mycelia biomass yield, both during growth on plates and in liquid media, promoted conidiation and increased the aflatoxin production. Furthermore, aflatoxin production increased with increased reactive oxidative species (ROS) levels at 96 h of growth, confirming illumination-driven oxidative stress modulation activity on <i>A. flavus</i> cells.https://www.mdpi.com/2072-6651/10/12/528VIS light illumination<i>Aspergillus flavus</i>mycotoxinsmycelial growthoxidative status modulationaflatoxin productionoptogenetics |
| spellingShingle | Tihomir Kovač Bojan Šarkanj Biljana Crevar Marija Kovač Ante Lončarić Ivica Strelec Chibundu N. Ezekiel Michael Sulyok Rudolf Krska <i>Aspergillus flavus</i> NRRL 3251 Growth, Oxidative Status, and Aflatoxins Production Ability <i>In Vitro</i> under Different Illumination Regimes Toxins VIS light illumination <i>Aspergillus flavus</i> mycotoxins mycelial growth oxidative status modulation aflatoxin production optogenetics |
| title | <i>Aspergillus flavus</i> NRRL 3251 Growth, Oxidative Status, and Aflatoxins Production Ability <i>In Vitro</i> under Different Illumination Regimes |
| title_full | <i>Aspergillus flavus</i> NRRL 3251 Growth, Oxidative Status, and Aflatoxins Production Ability <i>In Vitro</i> under Different Illumination Regimes |
| title_fullStr | <i>Aspergillus flavus</i> NRRL 3251 Growth, Oxidative Status, and Aflatoxins Production Ability <i>In Vitro</i> under Different Illumination Regimes |
| title_full_unstemmed | <i>Aspergillus flavus</i> NRRL 3251 Growth, Oxidative Status, and Aflatoxins Production Ability <i>In Vitro</i> under Different Illumination Regimes |
| title_short | <i>Aspergillus flavus</i> NRRL 3251 Growth, Oxidative Status, and Aflatoxins Production Ability <i>In Vitro</i> under Different Illumination Regimes |
| title_sort | i aspergillus flavus i nrrl 3251 growth oxidative status and aflatoxins production ability i in vitro i under different illumination regimes |
| topic | VIS light illumination <i>Aspergillus flavus</i> mycotoxins mycelial growth oxidative status modulation aflatoxin production optogenetics |
| url | https://www.mdpi.com/2072-6651/10/12/528 |
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