Applications of neutron reflectometry in biology
Over the last 10 years, neutron reflectometry (NR) has emerged as a powerful technique for the investigation of biologically relevant thin films. The great advantage of NR with respect to many other surface-sensitive techniques is its sub-nanometer resolution that enables structural characterization...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2020-01-01
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| Series: | EPJ Web of Conferences |
| Online Access: | https://www.epj-conferences.org/articles/epjconf/pdf/2020/12/epjconf_jdn24_04002.pdf |
| _version_ | 1819125607907196928 |
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| author | Gerelli Yuri |
| author_facet | Gerelli Yuri |
| author_sort | Gerelli Yuri |
| collection | DOAJ |
| description | Over the last 10 years, neutron reflectometry (NR) has emerged as a powerful technique for the investigation of biologically relevant thin films. The great advantage of NR with respect to many other surface-sensitive techniques is its sub-nanometer resolution that enables structural characterizations at the molecular level. In the case of bio-relevant samples, NR is non-destructive and can be used to probe thin films at buried interfaces or enclosed in bulky sample environment equipment. Moreover, recent advances in biomolecular deutera-tion enabled new labeling strategies to highlight certain structural features and to resolve with better accuracy the location of chemically similar molecules within a thin film.
In this chapter I will describe some applications of NR to bio-relevant samples and discuss some of the data analysis approaches available for biological thin films. In particular, examples on the structural characterization of biomembranes, protein films and protein-lipid interactions will be described. |
| first_indexed | 2024-12-22T07:42:50Z |
| format | Article |
| id | doaj.art-6e914501a87a4639acd6fecd9ff74e74 |
| institution | Directory Open Access Journal |
| issn | 2100-014X |
| language | English |
| last_indexed | 2024-12-22T07:42:50Z |
| publishDate | 2020-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | EPJ Web of Conferences |
| spelling | doaj.art-6e914501a87a4639acd6fecd9ff74e742022-12-21T18:33:42ZengEDP SciencesEPJ Web of Conferences2100-014X2020-01-012360400210.1051/epjconf/202023604002epjconf_jdn24_04002Applications of neutron reflectometry in biologyGerelli Yuri0Polytechnic University of Marche, Department of Life and Environmental SciencesOver the last 10 years, neutron reflectometry (NR) has emerged as a powerful technique for the investigation of biologically relevant thin films. The great advantage of NR with respect to many other surface-sensitive techniques is its sub-nanometer resolution that enables structural characterizations at the molecular level. In the case of bio-relevant samples, NR is non-destructive and can be used to probe thin films at buried interfaces or enclosed in bulky sample environment equipment. Moreover, recent advances in biomolecular deutera-tion enabled new labeling strategies to highlight certain structural features and to resolve with better accuracy the location of chemically similar molecules within a thin film. In this chapter I will describe some applications of NR to bio-relevant samples and discuss some of the data analysis approaches available for biological thin films. In particular, examples on the structural characterization of biomembranes, protein films and protein-lipid interactions will be described.https://www.epj-conferences.org/articles/epjconf/pdf/2020/12/epjconf_jdn24_04002.pdf |
| spellingShingle | Gerelli Yuri Applications of neutron reflectometry in biology EPJ Web of Conferences |
| title | Applications of neutron reflectometry in biology |
| title_full | Applications of neutron reflectometry in biology |
| title_fullStr | Applications of neutron reflectometry in biology |
| title_full_unstemmed | Applications of neutron reflectometry in biology |
| title_short | Applications of neutron reflectometry in biology |
| title_sort | applications of neutron reflectometry in biology |
| url | https://www.epj-conferences.org/articles/epjconf/pdf/2020/12/epjconf_jdn24_04002.pdf |
| work_keys_str_mv | AT gerelliyuri applicationsofneutronreflectometryinbiology |