Immunomodulatory Responses of Two Synthetic Peptides against <i>Salmonella</i> Typhimurium Infection
In vitro assays of phagocytic activity showed that the peptide Pin2[G] stimulates phagocytosis in BMDM cells from 0.15 to 1.25 μg/mL, and in RAW 264.7 cells at 0.31 μg/mL. In the same way, the peptide FA1 induced phagocytosis in BMDM cells from 1.17 to 4.69 μg/mL and in RAW 264.7 cells at 150 μg/mL....
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MDPI AG
2021-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/18/5573 |
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| author | Marco Antonio Ibarra-Valencia Gerardo Pável Espino-Solis Blanca Elisa Estrada Gerardo Corzo |
| author_facet | Marco Antonio Ibarra-Valencia Gerardo Pável Espino-Solis Blanca Elisa Estrada Gerardo Corzo |
| author_sort | Marco Antonio Ibarra-Valencia |
| collection | DOAJ |
| description | In vitro assays of phagocytic activity showed that the peptide Pin2[G] stimulates phagocytosis in BMDM cells from 0.15 to 1.25 μg/mL, and in RAW 264.7 cells at 0.31 μg/mL. In the same way, the peptide FA1 induced phagocytosis in BMDM cells from 1.17 to 4.69 μg/mL and in RAW 264.7 cells at 150 μg/mL. Cytokine profiles of uninfected RAW 264.7 showed that Pin2[G] increased liberation TNF (from 1.25 to 10 μg/mL) and MCP-1 (10 μg/mL), and FA1 also increased the release of TNF (from 18.75 to 75 μg/mL) but did not increase the liberation of MCP-1. In RAW 264.7 macrophages infected with <i>Salmonella enterica</i> serovar Typhimurium, the expression of TNF increases with Pin2[G] (1.25–10 μg/mL) or FA1 (18.75–75 μg/mL). In these cells, FA1 also increases the expression of IL-12p70, IL-10 and IFN-γ when applied at concentrations of 37.5, 75 and 150 μg/mL, respectively. On the other hand, stimulation with 1.25 and 10 μg/mL of Pin2[G] promotes the expression of MCP-1 and IL-12p70, respectively. Finally, peptides treatment did not resolve murine gastric infection, but improves their physical condition. Cytokine profiles showed that FA1 reduces IFN-γ and MCP-1 but increases IL-10, while Pin2[G] reduces IFN-γ but increases the liberation of IL-6 and IL-12p70. This data suggests a promising activity of FA1 and Pin2[G] as immunomodulators of gastric infections in <i>S.</i> Typhimurium. |
| first_indexed | 2024-03-10T07:24:09Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T07:24:09Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-6e92182001db4687b09340dd152969b02023-11-22T14:25:12ZengMDPI AGMolecules1420-30492021-09-012618557310.3390/molecules26185573Immunomodulatory Responses of Two Synthetic Peptides against <i>Salmonella</i> Typhimurium InfectionMarco Antonio Ibarra-Valencia0Gerardo Pável Espino-Solis1Blanca Elisa Estrada2Gerardo Corzo3Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, A.P. 510-3, Cuernavaca 62250, MexicoLaboratorio de Investigación Traslacional and Laboratorio Nacional de Citometría de Flujo-UACH, Universidad Autónoma de Chihuahua, Circuito Universitario, Campus II, Chihuahua 31109, MexicoLaboratorio de Investigación Traslacional and Laboratorio Nacional de Citometría de Flujo-UACH, Universidad Autónoma de Chihuahua, Circuito Universitario, Campus II, Chihuahua 31109, MexicoDepartamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, A.P. 510-3, Cuernavaca 62250, MexicoIn vitro assays of phagocytic activity showed that the peptide Pin2[G] stimulates phagocytosis in BMDM cells from 0.15 to 1.25 μg/mL, and in RAW 264.7 cells at 0.31 μg/mL. In the same way, the peptide FA1 induced phagocytosis in BMDM cells from 1.17 to 4.69 μg/mL and in RAW 264.7 cells at 150 μg/mL. Cytokine profiles of uninfected RAW 264.7 showed that Pin2[G] increased liberation TNF (from 1.25 to 10 μg/mL) and MCP-1 (10 μg/mL), and FA1 also increased the release of TNF (from 18.75 to 75 μg/mL) but did not increase the liberation of MCP-1. In RAW 264.7 macrophages infected with <i>Salmonella enterica</i> serovar Typhimurium, the expression of TNF increases with Pin2[G] (1.25–10 μg/mL) or FA1 (18.75–75 μg/mL). In these cells, FA1 also increases the expression of IL-12p70, IL-10 and IFN-γ when applied at concentrations of 37.5, 75 and 150 μg/mL, respectively. On the other hand, stimulation with 1.25 and 10 μg/mL of Pin2[G] promotes the expression of MCP-1 and IL-12p70, respectively. Finally, peptides treatment did not resolve murine gastric infection, but improves their physical condition. Cytokine profiles showed that FA1 reduces IFN-γ and MCP-1 but increases IL-10, while Pin2[G] reduces IFN-γ but increases the liberation of IL-6 and IL-12p70. This data suggests a promising activity of FA1 and Pin2[G] as immunomodulators of gastric infections in <i>S.</i> Typhimurium.https://www.mdpi.com/1420-3049/26/18/5573antimicrobial peptides<i>Salmonella enterica</i> serovar Typhimuriumcytokinesinflammationscorpion peptides |
| spellingShingle | Marco Antonio Ibarra-Valencia Gerardo Pável Espino-Solis Blanca Elisa Estrada Gerardo Corzo Immunomodulatory Responses of Two Synthetic Peptides against <i>Salmonella</i> Typhimurium Infection Molecules antimicrobial peptides <i>Salmonella enterica</i> serovar Typhimurium cytokines inflammation scorpion peptides |
| title | Immunomodulatory Responses of Two Synthetic Peptides against <i>Salmonella</i> Typhimurium Infection |
| title_full | Immunomodulatory Responses of Two Synthetic Peptides against <i>Salmonella</i> Typhimurium Infection |
| title_fullStr | Immunomodulatory Responses of Two Synthetic Peptides against <i>Salmonella</i> Typhimurium Infection |
| title_full_unstemmed | Immunomodulatory Responses of Two Synthetic Peptides against <i>Salmonella</i> Typhimurium Infection |
| title_short | Immunomodulatory Responses of Two Synthetic Peptides against <i>Salmonella</i> Typhimurium Infection |
| title_sort | immunomodulatory responses of two synthetic peptides against i salmonella i typhimurium infection |
| topic | antimicrobial peptides <i>Salmonella enterica</i> serovar Typhimurium cytokines inflammation scorpion peptides |
| url | https://www.mdpi.com/1420-3049/26/18/5573 |
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