<it>Clostridium difficile </it>genotypes other than ribotype 078 that are prevalent among human, animal and environmental isolates
<p>Abstract</p> <p>Background</p> <p>Characterising the overlap of <it>C. difficile </it>genotypes in different reservoirs can improve our understanding of possible transmission routes of this pathogen. Most of the studies have focused on a comparison of the...
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| Format: | Article |
| Language: | English |
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BMC
2012-03-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/12/48 |
| _version_ | 1819087041950908416 |
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| author | Janezic Sandra Ocepek Matjaz Zidaric Valerija Rupnik Maja |
| author_facet | Janezic Sandra Ocepek Matjaz Zidaric Valerija Rupnik Maja |
| author_sort | Janezic Sandra |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Characterising the overlap of <it>C. difficile </it>genotypes in different reservoirs can improve our understanding of possible transmission routes of this pathogen. Most of the studies have focused on a comparison of the PCR ribotype 078 isolated from humans and animals. Here we describe for the first time a comparison of <it>C. difficile </it>genotypes isolated during longer time intervals from different sources including humans, animals and the non-hospital environment.</p> <p>Results</p> <p>Altogether 786 isolates from time interval 2008-2010 were grouped into 90 PCR ribotypes and eleven of them were shared among all host types and the environment. Ribotypes that were most common in humans were also present in water and different animals (014/020, 002, 029). Interestingly, non-toxigenic isolates were very common in the environment (30.8%) in comparison to humans (6.5%) and animals (7.7%). A high degree of similarity was observed for human and animal isolates with PFGE. In human isolates resistance to erithromycin, clindamycin and moxifloxacin was detected, while all animal isolates were susceptible to all antibiotics tested.</p> <p>Conclusion</p> <p>Our results show that many other types in addition to PCR Ribotype 078 are shared between humans and animals and that the most prevalent genotypes in humans have the ability to survive also in the environment and several animal hosts. The genetic relatedness observed with PFGE suggests that transmission of given genotype from one reservoir to the other is likely to occur.</p> |
| first_indexed | 2024-12-21T21:29:51Z |
| format | Article |
| id | doaj.art-6e92da84789a4384a990f976af81692d |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-21T21:29:51Z |
| publishDate | 2012-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-6e92da84789a4384a990f976af81692d2022-12-21T18:49:39ZengBMCBMC Microbiology1471-21802012-03-011214810.1186/1471-2180-12-48<it>Clostridium difficile </it>genotypes other than ribotype 078 that are prevalent among human, animal and environmental isolatesJanezic SandraOcepek MatjazZidaric ValerijaRupnik Maja<p>Abstract</p> <p>Background</p> <p>Characterising the overlap of <it>C. difficile </it>genotypes in different reservoirs can improve our understanding of possible transmission routes of this pathogen. Most of the studies have focused on a comparison of the PCR ribotype 078 isolated from humans and animals. Here we describe for the first time a comparison of <it>C. difficile </it>genotypes isolated during longer time intervals from different sources including humans, animals and the non-hospital environment.</p> <p>Results</p> <p>Altogether 786 isolates from time interval 2008-2010 were grouped into 90 PCR ribotypes and eleven of them were shared among all host types and the environment. Ribotypes that were most common in humans were also present in water and different animals (014/020, 002, 029). Interestingly, non-toxigenic isolates were very common in the environment (30.8%) in comparison to humans (6.5%) and animals (7.7%). A high degree of similarity was observed for human and animal isolates with PFGE. In human isolates resistance to erithromycin, clindamycin and moxifloxacin was detected, while all animal isolates were susceptible to all antibiotics tested.</p> <p>Conclusion</p> <p>Our results show that many other types in addition to PCR Ribotype 078 are shared between humans and animals and that the most prevalent genotypes in humans have the ability to survive also in the environment and several animal hosts. The genetic relatedness observed with PFGE suggests that transmission of given genotype from one reservoir to the other is likely to occur.</p>http://www.biomedcentral.com/1471-2180/12/48 |
| spellingShingle | Janezic Sandra Ocepek Matjaz Zidaric Valerija Rupnik Maja <it>Clostridium difficile </it>genotypes other than ribotype 078 that are prevalent among human, animal and environmental isolates BMC Microbiology |
| title | <it>Clostridium difficile </it>genotypes other than ribotype 078 that are prevalent among human, animal and environmental isolates |
| title_full | <it>Clostridium difficile </it>genotypes other than ribotype 078 that are prevalent among human, animal and environmental isolates |
| title_fullStr | <it>Clostridium difficile </it>genotypes other than ribotype 078 that are prevalent among human, animal and environmental isolates |
| title_full_unstemmed | <it>Clostridium difficile </it>genotypes other than ribotype 078 that are prevalent among human, animal and environmental isolates |
| title_short | <it>Clostridium difficile </it>genotypes other than ribotype 078 that are prevalent among human, animal and environmental isolates |
| title_sort | it clostridium difficile it genotypes other than ribotype 078 that are prevalent among human animal and environmental isolates |
| url | http://www.biomedcentral.com/1471-2180/12/48 |
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