Sequential extraction of value-added bioproducts from three Chlorella strains using a drying-based combined disruption technique
Abstract In this study, the sequential extraction of the three types of biochemicals from microalgae is employed, which is a more realistic and practical solution for large-scale extraction of bioproducts. The drying, grinding, organic solvent treatment, and ultra-sonication were combined to disrupt...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-07-01
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| Series: | Bioresources and Bioprocessing |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40643-023-00664-1 |
| _version_ | 1797774418523455488 |
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| author | Zahra Izanlou Mahmood Akhavan Mahdavi Reza Gheshlaghi Arash Karimian |
| author_facet | Zahra Izanlou Mahmood Akhavan Mahdavi Reza Gheshlaghi Arash Karimian |
| author_sort | Zahra Izanlou |
| collection | DOAJ |
| description | Abstract In this study, the sequential extraction of the three types of biochemicals from microalgae is employed, which is a more realistic and practical solution for large-scale extraction of bioproducts. The drying, grinding, organic solvent treatment, and ultra-sonication were combined to disrupt cells and sequentially extract bioproducts from three microalgae strains, Chlorella sorokiniana IG-W-96, Chlorella sp. PG-96, and Chlorella vulgaris IG-R-96. As the drying is the most energy-intensive step in cell disruption and sequential extraction, the effect of this step on sequential extraction deeply explored. The results show that total ash-plus contents of biochemicals in freeze-dried samples (95.4 ± 2.8%, 89.3 ± 3.9%, and 77.5 ± 4.2 respectively) are higher than those in oven-dried samples (91.0 ± 2.8%, 89.5 ± 3.0%, 71.4 ± 4.8%, respectively) showing the superiority of freeze drying over oven drying merely for Chlorella vulgaris IG-R-96 (p-value = 0.003) and non-significant variation for Chlorella sorokiniana IG-W-96 (p-value = 0.085) and Chlorella sp. PG-96 (p-value = 0.466). Variation among biochemical contents of strains is due to the difference in cell wall strength confirmed by TEM imaging. The freeze-dried samples achieved higher lipid yields than oven-dried samples. The total carbohydrate yields followed the same pattern. The extraction yields of total protein were higher in freeze-dried samples than in oven-dried. Total mass balance revealed that drying-based sequential extraction of value-added bioproducts could better demonstrate the economic potential of sustainable and renewable algal feedstock than independent assays for each biochemical. Graphical Abstract |
| first_indexed | 2024-03-12T22:19:49Z |
| format | Article |
| id | doaj.art-6e92f585be9a4569b88f37b7ae604d36 |
| institution | Directory Open Access Journal |
| issn | 2197-4365 |
| language | English |
| last_indexed | 2024-03-12T22:19:49Z |
| publishDate | 2023-07-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bioresources and Bioprocessing |
| spelling | doaj.art-6e92f585be9a4569b88f37b7ae604d362023-07-23T11:05:19ZengSpringerOpenBioresources and Bioprocessing2197-43652023-07-0110111110.1186/s40643-023-00664-1Sequential extraction of value-added bioproducts from three Chlorella strains using a drying-based combined disruption techniqueZahra Izanlou0Mahmood Akhavan Mahdavi1Reza Gheshlaghi2Arash Karimian3Department of Chemical Engineering, Ferdowsi University of MashhadDepartment of Chemical Engineering, Ferdowsi University of MashhadDepartment of Chemical Engineering, Ferdowsi University of MashhadDepartment of Chemical Engineering, Ferdowsi University of MashhadAbstract In this study, the sequential extraction of the three types of biochemicals from microalgae is employed, which is a more realistic and practical solution for large-scale extraction of bioproducts. The drying, grinding, organic solvent treatment, and ultra-sonication were combined to disrupt cells and sequentially extract bioproducts from three microalgae strains, Chlorella sorokiniana IG-W-96, Chlorella sp. PG-96, and Chlorella vulgaris IG-R-96. As the drying is the most energy-intensive step in cell disruption and sequential extraction, the effect of this step on sequential extraction deeply explored. The results show that total ash-plus contents of biochemicals in freeze-dried samples (95.4 ± 2.8%, 89.3 ± 3.9%, and 77.5 ± 4.2 respectively) are higher than those in oven-dried samples (91.0 ± 2.8%, 89.5 ± 3.0%, 71.4 ± 4.8%, respectively) showing the superiority of freeze drying over oven drying merely for Chlorella vulgaris IG-R-96 (p-value = 0.003) and non-significant variation for Chlorella sorokiniana IG-W-96 (p-value = 0.085) and Chlorella sp. PG-96 (p-value = 0.466). Variation among biochemical contents of strains is due to the difference in cell wall strength confirmed by TEM imaging. The freeze-dried samples achieved higher lipid yields than oven-dried samples. The total carbohydrate yields followed the same pattern. The extraction yields of total protein were higher in freeze-dried samples than in oven-dried. Total mass balance revealed that drying-based sequential extraction of value-added bioproducts could better demonstrate the economic potential of sustainable and renewable algal feedstock than independent assays for each biochemical. Graphical Abstracthttps://doi.org/10.1186/s40643-023-00664-1ChlorellaBioproductsDisruptionDryingSequential extractionBiorefinery |
| spellingShingle | Zahra Izanlou Mahmood Akhavan Mahdavi Reza Gheshlaghi Arash Karimian Sequential extraction of value-added bioproducts from three Chlorella strains using a drying-based combined disruption technique Bioresources and Bioprocessing Chlorella Bioproducts Disruption Drying Sequential extraction Biorefinery |
| title | Sequential extraction of value-added bioproducts from three Chlorella strains using a drying-based combined disruption technique |
| title_full | Sequential extraction of value-added bioproducts from three Chlorella strains using a drying-based combined disruption technique |
| title_fullStr | Sequential extraction of value-added bioproducts from three Chlorella strains using a drying-based combined disruption technique |
| title_full_unstemmed | Sequential extraction of value-added bioproducts from three Chlorella strains using a drying-based combined disruption technique |
| title_short | Sequential extraction of value-added bioproducts from three Chlorella strains using a drying-based combined disruption technique |
| title_sort | sequential extraction of value added bioproducts from three chlorella strains using a drying based combined disruption technique |
| topic | Chlorella Bioproducts Disruption Drying Sequential extraction Biorefinery |
| url | https://doi.org/10.1186/s40643-023-00664-1 |
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