Ultrastructural heterogeneity of layer 4 excitatory synaptic boutons in the adult human temporal lobe neocortex
Synapses are fundamental building blocks controlling and modulating the ‘behavior’ of brain networks. How their structural composition, most notably their quantitative morphology underlie their computational properties remains rather unclear, particularly in humans. Here, excitatory synaptic boutons...
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eLife Sciences Publications Ltd
2019-11-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/48373 |
| _version_ | 1811200242009243648 |
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| author | Rachida Yakoubi Astrid Rollenhagen Marec von Lehe Dorothea Miller Bernd Walkenfort Mike Hasenberg Kurt Sätzler Joachim HR Lübke |
| author_facet | Rachida Yakoubi Astrid Rollenhagen Marec von Lehe Dorothea Miller Bernd Walkenfort Mike Hasenberg Kurt Sätzler Joachim HR Lübke |
| author_sort | Rachida Yakoubi |
| collection | DOAJ |
| description | Synapses are fundamental building blocks controlling and modulating the ‘behavior’ of brain networks. How their structural composition, most notably their quantitative morphology underlie their computational properties remains rather unclear, particularly in humans. Here, excitatory synaptic boutons (SBs) in layer 4 (L4) of the temporal lobe neocortex (TLN) were quantitatively investigated. Biopsies from epilepsy surgery were used for fine-scale and tomographic electron microscopy (EM) to generate 3D-reconstructions of SBs. Particularly, the size of active zones (AZs) and that of the three functionally defined pools of synaptic vesicles (SVs) were quantified. SBs were comparatively small (~2.50 μm2), with a single AZ (~0.13 µm2); preferentially established on spines. SBs had a total pool of ~1800 SVs with strikingly large readily releasable (~20), recycling (~80) and resting pools (~850). Thus, human L4 SBs may act as ‘amplifiers’ of signals from the sensory periphery, integrate, synchronize and modulate intra- and extracortical synaptic activity. |
| first_indexed | 2024-04-12T02:01:17Z |
| format | Article |
| id | doaj.art-6e937a74143141bfa6dd189f96221ced |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:01:17Z |
| publishDate | 2019-11-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-6e937a74143141bfa6dd189f96221ced2022-12-22T03:52:40ZengeLife Sciences Publications LtdeLife2050-084X2019-11-01810.7554/eLife.48373Ultrastructural heterogeneity of layer 4 excitatory synaptic boutons in the adult human temporal lobe neocortexRachida Yakoubi0Astrid Rollenhagen1Marec von Lehe2Dorothea Miller3Bernd Walkenfort4Mike Hasenberg5Kurt Sätzler6Joachim HR Lübke7https://orcid.org/0000-0002-4086-3199Institute of Neuroscience and Medicine INM-10, Research Centre Jülich GmbH, Jülich, GermanyInstitute of Neuroscience and Medicine INM-10, Research Centre Jülich GmbH, Jülich, GermanyDepartment of Neurosurgery, Knappschaftskrankenhaus Bochum, Bochum, Germany; Department of Neurosurgery, Brandenburg Medical School, Ruppiner Clinics, Neuruppin, GermanyDepartment of Neurosurgery, Knappschaftskrankenhaus Bochum, Bochum, GermanyMedical Research Centre, IMCES Electron Microscopy Unit (EMU), University Hospital Essen, Essen, GermanyMedical Research Centre, IMCES Electron Microscopy Unit (EMU), University Hospital Essen, Essen, GermanySchool of Biomedical Sciences, University of Ulster, Londonderry, United KingdomInstitute of Neuroscience and Medicine INM-10, Research Centre Jülich GmbH, Jülich, Germany; Department of Psychiatry, Psychotherapy and Psychosomatics, Faculty of Medicine, RWTH University Hospital Aachen, Aachen, Germany; JARA Translational Brain Medicine, Jülich/Aachen, GermanySynapses are fundamental building blocks controlling and modulating the ‘behavior’ of brain networks. How their structural composition, most notably their quantitative morphology underlie their computational properties remains rather unclear, particularly in humans. Here, excitatory synaptic boutons (SBs) in layer 4 (L4) of the temporal lobe neocortex (TLN) were quantitatively investigated. Biopsies from epilepsy surgery were used for fine-scale and tomographic electron microscopy (EM) to generate 3D-reconstructions of SBs. Particularly, the size of active zones (AZs) and that of the three functionally defined pools of synaptic vesicles (SVs) were quantified. SBs were comparatively small (~2.50 μm2), with a single AZ (~0.13 µm2); preferentially established on spines. SBs had a total pool of ~1800 SVs with strikingly large readily releasable (~20), recycling (~80) and resting pools (~850). Thus, human L4 SBs may act as ‘amplifiers’ of signals from the sensory periphery, integrate, synchronize and modulate intra- and extracortical synaptic activity.https://elifesciences.org/articles/48373synapseelectron microscopy3D-volume reconstructions |
| spellingShingle | Rachida Yakoubi Astrid Rollenhagen Marec von Lehe Dorothea Miller Bernd Walkenfort Mike Hasenberg Kurt Sätzler Joachim HR Lübke Ultrastructural heterogeneity of layer 4 excitatory synaptic boutons in the adult human temporal lobe neocortex eLife synapse electron microscopy 3D-volume reconstructions |
| title | Ultrastructural heterogeneity of layer 4 excitatory synaptic boutons in the adult human temporal lobe neocortex |
| title_full | Ultrastructural heterogeneity of layer 4 excitatory synaptic boutons in the adult human temporal lobe neocortex |
| title_fullStr | Ultrastructural heterogeneity of layer 4 excitatory synaptic boutons in the adult human temporal lobe neocortex |
| title_full_unstemmed | Ultrastructural heterogeneity of layer 4 excitatory synaptic boutons in the adult human temporal lobe neocortex |
| title_short | Ultrastructural heterogeneity of layer 4 excitatory synaptic boutons in the adult human temporal lobe neocortex |
| title_sort | ultrastructural heterogeneity of layer 4 excitatory synaptic boutons in the adult human temporal lobe neocortex |
| topic | synapse electron microscopy 3D-volume reconstructions |
| url | https://elifesciences.org/articles/48373 |
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