Discrimination and Detection of and with a Single Primer Set
Erwinia amylovora and Erwinia pyrifoliae cause fire blight and black-shoot blight, respectively, in apples and pears. E. pyrifoliae is less pathogenic and has a narrower host range than that of E. amylovora. Fire blight and black-shoot blight exhibit similar symptoms, making it difficult to distingu...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hanrimwon Publishing Company
2022-06-01
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| Series: | The Plant Pathology Journal |
| Subjects: | |
| Online Access: | http://www.ppjonline.org/upload/pdf/PPJ-OA-03-2022-0027.pdf |
| _version_ | 1798036979893403648 |
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| author | Hyeonheui Ham Kyongnim Kim Suin Yang Hyun Gi Kong Mi-Hyun Lee Yong Ju Jin Dong Suk Park |
| author_facet | Hyeonheui Ham Kyongnim Kim Suin Yang Hyun Gi Kong Mi-Hyun Lee Yong Ju Jin Dong Suk Park |
| author_sort | Hyeonheui Ham |
| collection | DOAJ |
| description | Erwinia amylovora and Erwinia pyrifoliae cause fire blight and black-shoot blight, respectively, in apples and pears. E. pyrifoliae is less pathogenic and has a narrower host range than that of E. amylovora. Fire blight and black-shoot blight exhibit similar symptoms, making it difficult to distinguish one bacterial disease from the other. Molecular tools that differentiate fire blight from black-shoot blight could guide in the implementation of appropriate management strategies to control both diseases. In this study, a primer set was developed to detect and distinguish E. amylovora from E. pyrifoliae by conventional polymerase chain reaction (PCR). The primers produced amplicons of different sizes that were specific to each bacterial species. PCR products from E. amylovora and E. pyrifoliae cells at concentrations of 104 cfu/ml and 107 cfu/ml, respectively, were amplified, which demonstrated sufficient primer detection sensitivity. This primer set provides a simple molecular tool to distinguish between two types of bacterial diseases with similar symptoms. |
| first_indexed | 2024-04-11T21:20:25Z |
| format | Article |
| id | doaj.art-6e9399885d6f4ca08a163bb032bbbff6 |
| institution | Directory Open Access Journal |
| issn | 1598-2254 2093-9280 |
| language | English |
| last_indexed | 2024-04-11T21:20:25Z |
| publishDate | 2022-06-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-6e9399885d6f4ca08a163bb032bbbff62022-12-22T04:02:39ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542093-92802022-06-0138319420210.5423/PPJ.OA.03.2022.00272313Discrimination and Detection of and with a Single Primer SetHyeonheui HamKyongnim KimSuin YangHyun Gi KongMi-Hyun LeeYong Ju JinDong Suk ParkErwinia amylovora and Erwinia pyrifoliae cause fire blight and black-shoot blight, respectively, in apples and pears. E. pyrifoliae is less pathogenic and has a narrower host range than that of E. amylovora. Fire blight and black-shoot blight exhibit similar symptoms, making it difficult to distinguish one bacterial disease from the other. Molecular tools that differentiate fire blight from black-shoot blight could guide in the implementation of appropriate management strategies to control both diseases. In this study, a primer set was developed to detect and distinguish E. amylovora from E. pyrifoliae by conventional polymerase chain reaction (PCR). The primers produced amplicons of different sizes that were specific to each bacterial species. PCR products from E. amylovora and E. pyrifoliae cells at concentrations of 104 cfu/ml and 107 cfu/ml, respectively, were amplified, which demonstrated sufficient primer detection sensitivity. This primer set provides a simple molecular tool to distinguish between two types of bacterial diseases with similar symptoms.http://www.ppjonline.org/upload/pdf/PPJ-OA-03-2022-0027.pdfdetectiondiscriminationpolymerase chain reaction |
| spellingShingle | Hyeonheui Ham Kyongnim Kim Suin Yang Hyun Gi Kong Mi-Hyun Lee Yong Ju Jin Dong Suk Park Discrimination and Detection of and with a Single Primer Set The Plant Pathology Journal detection discrimination polymerase chain reaction |
| title | Discrimination and Detection of and with a Single Primer Set |
| title_full | Discrimination and Detection of and with a Single Primer Set |
| title_fullStr | Discrimination and Detection of and with a Single Primer Set |
| title_full_unstemmed | Discrimination and Detection of and with a Single Primer Set |
| title_short | Discrimination and Detection of and with a Single Primer Set |
| title_sort | discrimination and detection of and with a single primer set |
| topic | detection discrimination polymerase chain reaction |
| url | http://www.ppjonline.org/upload/pdf/PPJ-OA-03-2022-0027.pdf |
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