Selection strategy of dextran sulfate sodium-induced acute or chronic colitis mouse models based on gut microbial profile
Abstract Background Dextran sulfate sodium (DSS) replicates ulcerative colitis (UC)-like colitis in murine models. However, the microbial characteristics of DSS-triggered colitis require further clarification. To analyze the changes in gut microbiota associated with DSS-induced acute and chronic col...
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| Language: | English |
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BMC
2021-10-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-021-02342-8 |
| _version_ | 1819148616311242752 |
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| author | Hao-Ming Xu Hong-Li Huang Yan-Di Liu Jia-Qi Zhu You-Lian Zhou Hui-Ting Chen Jing Xu Hai-Lan Zhao Xue Guo Wei Shi Yu-Qiang Nie Yong-Jian Zhou |
| author_facet | Hao-Ming Xu Hong-Li Huang Yan-Di Liu Jia-Qi Zhu You-Lian Zhou Hui-Ting Chen Jing Xu Hai-Lan Zhao Xue Guo Wei Shi Yu-Qiang Nie Yong-Jian Zhou |
| author_sort | Hao-Ming Xu |
| collection | DOAJ |
| description | Abstract Background Dextran sulfate sodium (DSS) replicates ulcerative colitis (UC)-like colitis in murine models. However, the microbial characteristics of DSS-triggered colitis require further clarification. To analyze the changes in gut microbiota associated with DSS-induced acute and chronic colitis. Methods Acute colitis was induced in mice by administering 3% DSS for 1 week in the drinking water, and chronic colitis was induced by supplementing drinking water with 2.5% DSS every other week for 5 weeks. Control groups received the same drinking water without DSS supplementation. The histopathological score and length of the colons, and disease activity index (DAI) were evaluated to confirm the presence of experimental colitis. Intestinal microbiota was profiled by 16S rDNA sequencing of cecal content. Results Mice with both acute and chronic DSS-triggered colitis had significantly higher DAI and colon histopathological scores in contrast to the control groups (P < 0.0001, P < 0.0001), and the colon was remarkably shortened (P < 0.0001, P < 0.0001). The gut microbiota α-diversity was partly downregulated in both acute and chronic colitis groups in contrast to their respective control groups (Pielou index P = 0.0022, P = 0.0649; Shannon index P = 0.0022, P = 0.0931). The reduction in the Pielou and Shannon indices were more obvious in mice with acute colitis (P = 0.0022, P = 0.0043). The relative abundance of Bacteroides and Turicibacter was increased (all P < 0.05), while that of Lachnospiraceae, Ruminococcaceae, Ruminiclostridium, Rikenella, Alistipes, Alloprevotella, and Butyricicoccus was significantly decreased after acute DSS induction (all P < 0.05). The relative abundance of Bacteroides, Akkermansia, Helicobacter, Parabacteroides, Erysipelatoclostridium, Turicibacter and Romboutsia was also markedly increased (all P < 0.05), and that of Lachnospiraceae_NK4A136_group, Alistipes, Enterorhabdus, Prevotellaceae_UCG-001, Butyricicoccus, Ruminiclostridium_6, Muribaculum, Ruminococcaceae_NK4A214_group, Family_XIII_UCG-001 and Flavonifractor was significantly decreased after chronic DSS induction (all P < 0.05). Conclusion DSS-induced acute and chronic colitis demonstrated similar symptoms and histopathological changes. The changes in the gut microbiota of the acute colitis model were closer to that observed in UC. The acute colitis model had greater abundance of SCFAs-producing bacteria and lower α-diversity compared to the chronic colitis model. |
| first_indexed | 2024-12-22T13:48:33Z |
| format | Article |
| id | doaj.art-6e9ddfaad63a4d038ae091db09064fc2 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-22T13:48:33Z |
| publishDate | 2021-10-01 |
| publisher | BMC |
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| series | BMC Microbiology |
| spelling | doaj.art-6e9ddfaad63a4d038ae091db09064fc22022-12-21T18:23:43ZengBMCBMC Microbiology1471-21802021-10-0121111410.1186/s12866-021-02342-8Selection strategy of dextran sulfate sodium-induced acute or chronic colitis mouse models based on gut microbial profileHao-Ming Xu0Hong-Li Huang1Yan-Di Liu2Jia-Qi Zhu3You-Lian Zhou4Hui-Ting Chen5Jing Xu6Hai-Lan Zhao7Xue Guo8Wei Shi9Yu-Qiang Nie10Yong-Jian Zhou11Department of Gastroenterology and Hepatology, Guangzhou Digestive Disease Center, Guangzhou First People’s Hospital, School of Medicine, South China University of TechnologyDepartment of Gastroenterology and Hepatology, Guangzhou Digestive Disease Center, Guangzhou First People’s Hospital, School of Medicine, South China University of TechnologyDepartment of Gastroenterology and Hepatology, Guangzhou Digestive Disease Center, Guangzhou First People’s Hospital, School of Medicine, South China University of TechnologyDepartment of Gastroenterology and Hepatology, Guangzhou Digestive Disease Center, Guangzhou First People’s Hospital, School of Medicine, South China University of TechnologyDepartment of Gastroenterology and Hepatology, Guangzhou Digestive Disease Center, Guangzhou First People’s Hospital, School of Medicine, South China University of TechnologyDepartment of Gastroenterology and Hepatology, Guangzhou Digestive Disease Center, Guangzhou First People’s Hospital, School of Medicine, South China University of TechnologyDepartment of Gastroenterology and Hepatology, Guangzhou Digestive Disease Center, Guangzhou First People’s Hospital, School of Medicine, South China University of TechnologyDepartment of Gastroenterology and Hepatology, Guangzhou Digestive Disease Center, Guangzhou First People’s Hospital, School of Medicine, South China University of TechnologyDepartment of Gastroenterology and Hepatology, Guangzhou Digestive Disease Center, Guangzhou First People’s Hospital, School of Medicine, South China University of TechnologyDepartment of Geriatrics, Guangzhou First People’s Hospital, School of Medicine, South China University of TechnologyDepartment of Gastroenterology and Hepatology, Guangzhou Digestive Disease Center, Guangzhou First People’s Hospital, School of Medicine, South China University of TechnologyDepartment of Gastroenterology and Hepatology, Guangzhou Digestive Disease Center, Guangzhou First People’s Hospital, School of Medicine, South China University of TechnologyAbstract Background Dextran sulfate sodium (DSS) replicates ulcerative colitis (UC)-like colitis in murine models. However, the microbial characteristics of DSS-triggered colitis require further clarification. To analyze the changes in gut microbiota associated with DSS-induced acute and chronic colitis. Methods Acute colitis was induced in mice by administering 3% DSS for 1 week in the drinking water, and chronic colitis was induced by supplementing drinking water with 2.5% DSS every other week for 5 weeks. Control groups received the same drinking water without DSS supplementation. The histopathological score and length of the colons, and disease activity index (DAI) were evaluated to confirm the presence of experimental colitis. Intestinal microbiota was profiled by 16S rDNA sequencing of cecal content. Results Mice with both acute and chronic DSS-triggered colitis had significantly higher DAI and colon histopathological scores in contrast to the control groups (P < 0.0001, P < 0.0001), and the colon was remarkably shortened (P < 0.0001, P < 0.0001). The gut microbiota α-diversity was partly downregulated in both acute and chronic colitis groups in contrast to their respective control groups (Pielou index P = 0.0022, P = 0.0649; Shannon index P = 0.0022, P = 0.0931). The reduction in the Pielou and Shannon indices were more obvious in mice with acute colitis (P = 0.0022, P = 0.0043). The relative abundance of Bacteroides and Turicibacter was increased (all P < 0.05), while that of Lachnospiraceae, Ruminococcaceae, Ruminiclostridium, Rikenella, Alistipes, Alloprevotella, and Butyricicoccus was significantly decreased after acute DSS induction (all P < 0.05). The relative abundance of Bacteroides, Akkermansia, Helicobacter, Parabacteroides, Erysipelatoclostridium, Turicibacter and Romboutsia was also markedly increased (all P < 0.05), and that of Lachnospiraceae_NK4A136_group, Alistipes, Enterorhabdus, Prevotellaceae_UCG-001, Butyricicoccus, Ruminiclostridium_6, Muribaculum, Ruminococcaceae_NK4A214_group, Family_XIII_UCG-001 and Flavonifractor was significantly decreased after chronic DSS induction (all P < 0.05). Conclusion DSS-induced acute and chronic colitis demonstrated similar symptoms and histopathological changes. The changes in the gut microbiota of the acute colitis model were closer to that observed in UC. The acute colitis model had greater abundance of SCFAs-producing bacteria and lower α-diversity compared to the chronic colitis model.https://doi.org/10.1186/s12866-021-02342-8Dextran sulfate sodiumAcute colitisChronic colitisGut microbiota |
| spellingShingle | Hao-Ming Xu Hong-Li Huang Yan-Di Liu Jia-Qi Zhu You-Lian Zhou Hui-Ting Chen Jing Xu Hai-Lan Zhao Xue Guo Wei Shi Yu-Qiang Nie Yong-Jian Zhou Selection strategy of dextran sulfate sodium-induced acute or chronic colitis mouse models based on gut microbial profile BMC Microbiology Dextran sulfate sodium Acute colitis Chronic colitis Gut microbiota |
| title | Selection strategy of dextran sulfate sodium-induced acute or chronic colitis mouse models based on gut microbial profile |
| title_full | Selection strategy of dextran sulfate sodium-induced acute or chronic colitis mouse models based on gut microbial profile |
| title_fullStr | Selection strategy of dextran sulfate sodium-induced acute or chronic colitis mouse models based on gut microbial profile |
| title_full_unstemmed | Selection strategy of dextran sulfate sodium-induced acute or chronic colitis mouse models based on gut microbial profile |
| title_short | Selection strategy of dextran sulfate sodium-induced acute or chronic colitis mouse models based on gut microbial profile |
| title_sort | selection strategy of dextran sulfate sodium induced acute or chronic colitis mouse models based on gut microbial profile |
| topic | Dextran sulfate sodium Acute colitis Chronic colitis Gut microbiota |
| url | https://doi.org/10.1186/s12866-021-02342-8 |
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