Rapid Creation of New Photoperiod-/Thermo-Sensitive Genic Male-Sterile Rice Materials by CRISPR/Cas9 System
SUMMARY: Rice is an important food crop in China, and the development of hybrid rice is a crucial way to increase grain yield. The creation of dual-purpose nuclear-sterile lines for two-line hybrid breeding has become vital for commercial rice breeding. We constructed the pC1300-2x35S::Cas9-sgRNAPTG...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019-03-01
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| Series: | Rice Science |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1672630819300083 |
| _version_ | 1818512385194852352 |
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| author | Shen Lan Dong Guojun Zhang Yu Hu Guocheng Zhang Qiang Hu Guanglian Xu Bo Ren Deyong Hu Jiang Zhu Li Gao Zhenyu Zhang Guangheng Guo Longbiao Zeng Dali Qian Qian |
| author_facet | Shen Lan Dong Guojun Zhang Yu Hu Guocheng Zhang Qiang Hu Guanglian Xu Bo Ren Deyong Hu Jiang Zhu Li Gao Zhenyu Zhang Guangheng Guo Longbiao Zeng Dali Qian Qian |
| author_sort | Shen Lan |
| collection | DOAJ |
| description | SUMMARY: Rice is an important food crop in China, and the development of hybrid rice is a crucial way to increase grain yield. The creation of dual-purpose nuclear-sterile lines for two-line hybrid breeding has become vital for commercial rice breeding. We constructed the pC1300-2x35S::Cas9-sgRNAPTGMS2-1 expression vector for editing the male fertility gene PTGMS2-1 in two widely compatible rice varieties, 93-11 and Huazhan, by using the CRISPR/Cas9 system. We obtained the marker-free photoperiod-/thermo- sensitive genic male-sterile (P/TGMS) lines in T1 generation. According to the experiments in phytotron with four temperature and photoperiod treatments, we found the temperature is the main factor for restoring the pollen fertility of ptgms2-1 mutants in 93-11 and Huazhan, and the photoperiod also has some effects on pollen fertility in two different rice backgrounds. The application of cultivating new male-sterile lines by genome editing system will significantly accelerate the rice breeding process. |
| first_indexed | 2024-12-10T23:46:06Z |
| format | Article |
| id | doaj.art-6e9eeff0fc3d440c8ddf61d2a8abdff6 |
| institution | Directory Open Access Journal |
| issn | 1672-6308 |
| language | English |
| last_indexed | 2024-12-10T23:46:06Z |
| publishDate | 2019-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Rice Science |
| spelling | doaj.art-6e9eeff0fc3d440c8ddf61d2a8abdff62022-12-22T01:28:55ZengElsevierRice Science1672-63082019-03-01262129132Rapid Creation of New Photoperiod-/Thermo-Sensitive Genic Male-Sterile Rice Materials by CRISPR/Cas9 SystemShen Lan0Dong Guojun1Zhang Yu2Hu Guocheng3Zhang Qiang4Hu Guanglian5Xu Bo6Ren Deyong7Hu Jiang8Zhu Li9Gao Zhenyu10Zhang Guangheng11Guo Longbiao12Zeng Dali13Qian Qian14State Key Laboratory of Rice Biology/China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310006, ChinaState Key Laboratory of Rice Biology/China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310006, ChinaState Key Laboratory of Rice Biology/China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310006, ChinaState Key Laboratory of Rice Biology/China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310006, ChinaState Key Laboratory of Rice Biology/China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310006, ChinaState Key Laboratory of Rice Biology/China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310006, ChinaState Key Laboratory of Rice Biology/China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310006, ChinaState Key Laboratory of Rice Biology/China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310006, ChinaState Key Laboratory of Rice Biology/China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310006, ChinaState Key Laboratory of Rice Biology/China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310006, ChinaState Key Laboratory of Rice Biology/China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310006, ChinaState Key Laboratory of Rice Biology/China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310006, ChinaState Key Laboratory of Rice Biology/China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310006, ChinaState Key Laboratory of Rice Biology/China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310006, ChinaCorresponding author.; State Key Laboratory of Rice Biology/China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310006, ChinaSUMMARY: Rice is an important food crop in China, and the development of hybrid rice is a crucial way to increase grain yield. The creation of dual-purpose nuclear-sterile lines for two-line hybrid breeding has become vital for commercial rice breeding. We constructed the pC1300-2x35S::Cas9-sgRNAPTGMS2-1 expression vector for editing the male fertility gene PTGMS2-1 in two widely compatible rice varieties, 93-11 and Huazhan, by using the CRISPR/Cas9 system. We obtained the marker-free photoperiod-/thermo- sensitive genic male-sterile (P/TGMS) lines in T1 generation. According to the experiments in phytotron with four temperature and photoperiod treatments, we found the temperature is the main factor for restoring the pollen fertility of ptgms2-1 mutants in 93-11 and Huazhan, and the photoperiod also has some effects on pollen fertility in two different rice backgrounds. The application of cultivating new male-sterile lines by genome editing system will significantly accelerate the rice breeding process.http://www.sciencedirect.com/science/article/pii/S1672630819300083 |
| spellingShingle | Shen Lan Dong Guojun Zhang Yu Hu Guocheng Zhang Qiang Hu Guanglian Xu Bo Ren Deyong Hu Jiang Zhu Li Gao Zhenyu Zhang Guangheng Guo Longbiao Zeng Dali Qian Qian Rapid Creation of New Photoperiod-/Thermo-Sensitive Genic Male-Sterile Rice Materials by CRISPR/Cas9 System Rice Science |
| title | Rapid Creation of New Photoperiod-/Thermo-Sensitive Genic Male-Sterile Rice Materials by CRISPR/Cas9 System |
| title_full | Rapid Creation of New Photoperiod-/Thermo-Sensitive Genic Male-Sterile Rice Materials by CRISPR/Cas9 System |
| title_fullStr | Rapid Creation of New Photoperiod-/Thermo-Sensitive Genic Male-Sterile Rice Materials by CRISPR/Cas9 System |
| title_full_unstemmed | Rapid Creation of New Photoperiod-/Thermo-Sensitive Genic Male-Sterile Rice Materials by CRISPR/Cas9 System |
| title_short | Rapid Creation of New Photoperiod-/Thermo-Sensitive Genic Male-Sterile Rice Materials by CRISPR/Cas9 System |
| title_sort | rapid creation of new photoperiod thermo sensitive genic male sterile rice materials by crispr cas9 system |
| url | http://www.sciencedirect.com/science/article/pii/S1672630819300083 |
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