In Vitro Comparative Study of Near-Infrared Photoimmunotherapy and Photodynamic Therapy
Near-infrared photoimmunotherapy (NIR-PIT) is a new phototherapy that utilizes a monoclonal antibody (mAb) against cancer antigens and a phthalocyanine dye, IRDye700DX (IR700) conjugate (mAb-IR700). Photodynamic therapy (PDT) is a combination therapy that utilizes photoreactive agents and light irra...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/15/13/3400 |
| _version_ | 1797591974325256192 |
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| author | Susumu Yamashita Miho Kojima Nobuhiko Onda Makoto Shibutani |
| author_facet | Susumu Yamashita Miho Kojima Nobuhiko Onda Makoto Shibutani |
| author_sort | Susumu Yamashita |
| collection | DOAJ |
| description | Near-infrared photoimmunotherapy (NIR-PIT) is a new phototherapy that utilizes a monoclonal antibody (mAb) against cancer antigens and a phthalocyanine dye, IRDye700DX (IR700) conjugate (mAb-IR700). Photodynamic therapy (PDT) is a combination therapy that utilizes photoreactive agents and light irradiation as well as NIR-PIT. In the present study, we compared these therapies in vitro. The characterization of cellular binding/uptake specificity and cytotoxicity were examined using two mAb-IR700 forms and a conventional PDT agent, talaporfin sodium, in three cell lines. As designed, mAb-IR700 had high molecular selectivity and visualized target molecule-positive cells at the lowest concentration examined. NIR-PIT induced necrosis and damage-associated molecular patterns (DAMPs), a surrogate maker of immunogenic cell death. In contrast, talaporfin sodium was taken up by cells regardless of cell type, and its uptake was enhanced in a concentration-dependent manner. PDT induced cell death, with the pattern of cell death shifting from apoptosis to necrosis depending on the concentration of the photosensitizer. Induction of DAMPs was observed at the highest concentration, but their sensitivity differed among cell lines. Overall, our data suggest that molecule-specific NIR-PIT may have potential advantages compared with PDT in terms of the efficiency of tumor visualization and induction of DAMPs. |
| first_indexed | 2024-03-11T01:45:13Z |
| format | Article |
| id | doaj.art-6ea37fcaf18d41f38a76188ab428578a |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-11T01:45:13Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-6ea37fcaf18d41f38a76188ab428578a2023-11-18T16:16:28ZengMDPI AGCancers2072-66942023-06-011513340010.3390/cancers15133400In Vitro Comparative Study of Near-Infrared Photoimmunotherapy and Photodynamic TherapySusumu Yamashita0Miho Kojima1Nobuhiko Onda2Makoto Shibutani3Laboratory of Veterinary Pathology, Division of Animal Life Science, Institute of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu 183-8509, Tokyo, JapanMedical Evaluation Engineering, Olympus Medical Systems Corporation, 2-3 Kuboyama-cho, Hachioji 192-8512, Tokyo, JapanMedical Evaluation Engineering, Olympus Medical Systems Corporation, 2-3 Kuboyama-cho, Hachioji 192-8512, Tokyo, JapanLaboratory of Veterinary Pathology, Division of Animal Life Science, Institute of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu 183-8509, Tokyo, JapanNear-infrared photoimmunotherapy (NIR-PIT) is a new phototherapy that utilizes a monoclonal antibody (mAb) against cancer antigens and a phthalocyanine dye, IRDye700DX (IR700) conjugate (mAb-IR700). Photodynamic therapy (PDT) is a combination therapy that utilizes photoreactive agents and light irradiation as well as NIR-PIT. In the present study, we compared these therapies in vitro. The characterization of cellular binding/uptake specificity and cytotoxicity were examined using two mAb-IR700 forms and a conventional PDT agent, talaporfin sodium, in three cell lines. As designed, mAb-IR700 had high molecular selectivity and visualized target molecule-positive cells at the lowest concentration examined. NIR-PIT induced necrosis and damage-associated molecular patterns (DAMPs), a surrogate maker of immunogenic cell death. In contrast, talaporfin sodium was taken up by cells regardless of cell type, and its uptake was enhanced in a concentration-dependent manner. PDT induced cell death, with the pattern of cell death shifting from apoptosis to necrosis depending on the concentration of the photosensitizer. Induction of DAMPs was observed at the highest concentration, but their sensitivity differed among cell lines. Overall, our data suggest that molecule-specific NIR-PIT may have potential advantages compared with PDT in terms of the efficiency of tumor visualization and induction of DAMPs.https://www.mdpi.com/2072-6694/15/13/3400near-infrared photoimmunotherapy (NIR-PIT)monoclonal antibody (mAb)IRDye700photodynamic therapy (PDT)talaporfin sodium |
| spellingShingle | Susumu Yamashita Miho Kojima Nobuhiko Onda Makoto Shibutani In Vitro Comparative Study of Near-Infrared Photoimmunotherapy and Photodynamic Therapy Cancers near-infrared photoimmunotherapy (NIR-PIT) monoclonal antibody (mAb) IRDye700 photodynamic therapy (PDT) talaporfin sodium |
| title | In Vitro Comparative Study of Near-Infrared Photoimmunotherapy and Photodynamic Therapy |
| title_full | In Vitro Comparative Study of Near-Infrared Photoimmunotherapy and Photodynamic Therapy |
| title_fullStr | In Vitro Comparative Study of Near-Infrared Photoimmunotherapy and Photodynamic Therapy |
| title_full_unstemmed | In Vitro Comparative Study of Near-Infrared Photoimmunotherapy and Photodynamic Therapy |
| title_short | In Vitro Comparative Study of Near-Infrared Photoimmunotherapy and Photodynamic Therapy |
| title_sort | in vitro comparative study of near infrared photoimmunotherapy and photodynamic therapy |
| topic | near-infrared photoimmunotherapy (NIR-PIT) monoclonal antibody (mAb) IRDye700 photodynamic therapy (PDT) talaporfin sodium |
| url | https://www.mdpi.com/2072-6694/15/13/3400 |
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