Failure to Remove Bluetongue Serotype 8 Virus (BTV-8) From in vitro Produced and in vivo Derived Bovine Embryos and Subsequent Transmission of BTV-8 to Recipient Cows After Embryo Transfer
The behavior of BTV-8 in cattle is different from most other serotypes not only with regards to clinical signs but certainly with respect to virus transmission (transplacental, contact). Therefore, the possibility of virus transmission by means of embryo transfer was examined by in vitro exposure of...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2019-12-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fvets.2019.00432/full |
| _version_ | 1819009667584491520 |
|---|---|
| author | Andy Haegeman Leen Vandaele Ilse De Leeuw André P. Oliveira André P. Oliveira Hans Nauwynck Ann Van Soom Kris De Clercq |
| author_facet | Andy Haegeman Leen Vandaele Ilse De Leeuw André P. Oliveira André P. Oliveira Hans Nauwynck Ann Van Soom Kris De Clercq |
| author_sort | Andy Haegeman |
| collection | DOAJ |
| description | The behavior of BTV-8 in cattle is different from most other serotypes not only with regards to clinical signs but certainly with respect to virus transmission (transplacental, contact). Therefore, the possibility of virus transmission by means of embryo transfer was examined by in vitro exposure of in vitro produced and in vivo derived bovine blastocysts to BTV-8 followed by different washing protocols, including longer exposure times (up to 120 s) to 0.25% trypsin at room temperature or at 37°C. None of the washing protocols used was successful in removing the viral genome completely from the in vitro produced and in vivo derived embryos as was demonstrated by real-time PCR. Moreover, BTV-8 virus was transmitted to recipient cows after embryo transfer of in vivo derived BTV8-exposed embryos, which had been subjected to routine decontamination as recommended by IETS, consisting of 5 washes in PBS followed by a double treatment of 0.25% trypsin for 45s at 37°C, and an additional 5 washes in PBS with 2% FCS. This study clearly demonstrates the necessity of vigorous application of the directives for screening of potential donors and the collected embryos, especially in regions with BTV-8, to prevent transmission of the disease. |
| first_indexed | 2024-12-21T01:00:01Z |
| format | Article |
| id | doaj.art-6eaa5ec324204b54b0f2e866e3c117d8 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-21T01:00:01Z |
| publishDate | 2019-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-6eaa5ec324204b54b0f2e866e3c117d82022-12-21T19:21:12ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692019-12-01610.3389/fvets.2019.00432503753Failure to Remove Bluetongue Serotype 8 Virus (BTV-8) From in vitro Produced and in vivo Derived Bovine Embryos and Subsequent Transmission of BTV-8 to Recipient Cows After Embryo TransferAndy Haegeman0Leen Vandaele1Ilse De Leeuw2André P. Oliveira3André P. Oliveira4Hans Nauwynck5Ann Van Soom6Kris De Clercq7Unit of Exotic and Particular Diseases, Sciensano, Brussels, BelgiumDepartment of Reproduction, Obstetrics and Herd Health, Ghent University, Merelbeke, BelgiumUnit of Exotic and Particular Diseases, Sciensano, Brussels, BelgiumDepartment of Reproduction, Obstetrics and Herd Health, Ghent University, Merelbeke, BelgiumEPAMIG, Escola de Veterinaria da UFMG, Bolsista CAPES, Belo Horizonte, BrazilLaboratory of Virology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, BelgiumDepartment of Reproduction, Obstetrics and Herd Health, Ghent University, Merelbeke, BelgiumUnit of Exotic and Particular Diseases, Sciensano, Brussels, BelgiumThe behavior of BTV-8 in cattle is different from most other serotypes not only with regards to clinical signs but certainly with respect to virus transmission (transplacental, contact). Therefore, the possibility of virus transmission by means of embryo transfer was examined by in vitro exposure of in vitro produced and in vivo derived bovine blastocysts to BTV-8 followed by different washing protocols, including longer exposure times (up to 120 s) to 0.25% trypsin at room temperature or at 37°C. None of the washing protocols used was successful in removing the viral genome completely from the in vitro produced and in vivo derived embryos as was demonstrated by real-time PCR. Moreover, BTV-8 virus was transmitted to recipient cows after embryo transfer of in vivo derived BTV8-exposed embryos, which had been subjected to routine decontamination as recommended by IETS, consisting of 5 washes in PBS followed by a double treatment of 0.25% trypsin for 45s at 37°C, and an additional 5 washes in PBS with 2% FCS. This study clearly demonstrates the necessity of vigorous application of the directives for screening of potential donors and the collected embryos, especially in regions with BTV-8, to prevent transmission of the disease.https://www.frontiersin.org/article/10.3389/fvets.2019.00432/fullbovine embryoIETS guidelinesBluetongue virusBTV-8transmission |
| spellingShingle | Andy Haegeman Leen Vandaele Ilse De Leeuw André P. Oliveira André P. Oliveira Hans Nauwynck Ann Van Soom Kris De Clercq Failure to Remove Bluetongue Serotype 8 Virus (BTV-8) From in vitro Produced and in vivo Derived Bovine Embryos and Subsequent Transmission of BTV-8 to Recipient Cows After Embryo Transfer Frontiers in Veterinary Science bovine embryo IETS guidelines Bluetongue virus BTV-8 transmission |
| title | Failure to Remove Bluetongue Serotype 8 Virus (BTV-8) From in vitro Produced and in vivo Derived Bovine Embryos and Subsequent Transmission of BTV-8 to Recipient Cows After Embryo Transfer |
| title_full | Failure to Remove Bluetongue Serotype 8 Virus (BTV-8) From in vitro Produced and in vivo Derived Bovine Embryos and Subsequent Transmission of BTV-8 to Recipient Cows After Embryo Transfer |
| title_fullStr | Failure to Remove Bluetongue Serotype 8 Virus (BTV-8) From in vitro Produced and in vivo Derived Bovine Embryos and Subsequent Transmission of BTV-8 to Recipient Cows After Embryo Transfer |
| title_full_unstemmed | Failure to Remove Bluetongue Serotype 8 Virus (BTV-8) From in vitro Produced and in vivo Derived Bovine Embryos and Subsequent Transmission of BTV-8 to Recipient Cows After Embryo Transfer |
| title_short | Failure to Remove Bluetongue Serotype 8 Virus (BTV-8) From in vitro Produced and in vivo Derived Bovine Embryos and Subsequent Transmission of BTV-8 to Recipient Cows After Embryo Transfer |
| title_sort | failure to remove bluetongue serotype 8 virus btv 8 from in vitro produced and in vivo derived bovine embryos and subsequent transmission of btv 8 to recipient cows after embryo transfer |
| topic | bovine embryo IETS guidelines Bluetongue virus BTV-8 transmission |
| url | https://www.frontiersin.org/article/10.3389/fvets.2019.00432/full |
| work_keys_str_mv | AT andyhaegeman failuretoremovebluetongueserotype8virusbtv8frominvitroproducedandinvivoderivedbovineembryosandsubsequenttransmissionofbtv8torecipientcowsafterembryotransfer AT leenvandaele failuretoremovebluetongueserotype8virusbtv8frominvitroproducedandinvivoderivedbovineembryosandsubsequenttransmissionofbtv8torecipientcowsafterembryotransfer AT ilsedeleeuw failuretoremovebluetongueserotype8virusbtv8frominvitroproducedandinvivoderivedbovineembryosandsubsequenttransmissionofbtv8torecipientcowsafterembryotransfer AT andrepoliveira failuretoremovebluetongueserotype8virusbtv8frominvitroproducedandinvivoderivedbovineembryosandsubsequenttransmissionofbtv8torecipientcowsafterembryotransfer AT andrepoliveira failuretoremovebluetongueserotype8virusbtv8frominvitroproducedandinvivoderivedbovineembryosandsubsequenttransmissionofbtv8torecipientcowsafterembryotransfer AT hansnauwynck failuretoremovebluetongueserotype8virusbtv8frominvitroproducedandinvivoderivedbovineembryosandsubsequenttransmissionofbtv8torecipientcowsafterembryotransfer AT annvansoom failuretoremovebluetongueserotype8virusbtv8frominvitroproducedandinvivoderivedbovineembryosandsubsequenttransmissionofbtv8torecipientcowsafterembryotransfer AT krisdeclercq failuretoremovebluetongueserotype8virusbtv8frominvitroproducedandinvivoderivedbovineembryosandsubsequenttransmissionofbtv8torecipientcowsafterembryotransfer |