A Single-Center Experience on HLA Typing with 11 Loci Next Generation Sequencing in Korean Patients with Hematologic Disease
The human leukocyte antigen (<i>HLA</i>) system comprises the most polymorphic genes of the human genome and is famous for its potential pathological roles. To accurately type HLA genes and find HLA-matched donors, which are critical for effective hematopoietic transplantation, HLA typin...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/12/5/1074 |
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| author | Namsoo Kim Sinyoung Kim Jong Rak Choi Younhee Park |
| author_facet | Namsoo Kim Sinyoung Kim Jong Rak Choi Younhee Park |
| author_sort | Namsoo Kim |
| collection | DOAJ |
| description | The human leukocyte antigen (<i>HLA</i>) system comprises the most polymorphic genes of the human genome and is famous for its potential pathological roles. To accurately type HLA genes and find HLA-matched donors, which are critical for effective hematopoietic transplantation, HLA typing using next-generation sequencing (NGS) was implemented. We aimed to share the experience of HLA typing using NGS in patients with hematologic malignancies and evaluate its association with hematologic diseases. Data from 211 Korean, non-familial patients diagnosed with a hematologic disease were reviewed, and NGS was performed for 11 <i>HLA</i> loci. Three-field HLA typing with G code was successfully achieved for all loci and the known linkage between <i>HLA-DRB3/4/5</i> and <i>HLA-DRB1</i> was fully matched. Therefore, NGS-based HLA typing enables a detailed, high-resolution analysis of the HLA system that can help with the selection of suitable donors. Notably, <i>HLA-DRB1*08:02:01G</i> was significantly associated with myelodysplastic syndrome. Although this result confirms the tendency of some alleles to be associated with hematological disorders, this may not be the case in hematologic malignancies. Nonetheless, NGS-based HLA typing data for <i>HLA-DP</i>, <i>HLA-DQ</i>, and <i>HLA-DRB3</i>/4/5 are still warranted for a better understanding of the corresponding locus. |
| first_indexed | 2024-03-10T03:02:55Z |
| format | Article |
| id | doaj.art-6eae3565b91144e788343d29aa4dd05d |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T03:02:55Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-6eae3565b91144e788343d29aa4dd05d2023-11-23T10:38:58ZengMDPI AGDiagnostics2075-44182022-04-01125107410.3390/diagnostics12051074A Single-Center Experience on HLA Typing with 11 Loci Next Generation Sequencing in Korean Patients with Hematologic DiseaseNamsoo Kim0Sinyoung Kim1Jong Rak Choi2Younhee Park3Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul 03722, KoreaDepartment of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul 03722, KoreaDepartment of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul 03722, KoreaDepartment of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul 03722, KoreaThe human leukocyte antigen (<i>HLA</i>) system comprises the most polymorphic genes of the human genome and is famous for its potential pathological roles. To accurately type HLA genes and find HLA-matched donors, which are critical for effective hematopoietic transplantation, HLA typing using next-generation sequencing (NGS) was implemented. We aimed to share the experience of HLA typing using NGS in patients with hematologic malignancies and evaluate its association with hematologic diseases. Data from 211 Korean, non-familial patients diagnosed with a hematologic disease were reviewed, and NGS was performed for 11 <i>HLA</i> loci. Three-field HLA typing with G code was successfully achieved for all loci and the known linkage between <i>HLA-DRB3/4/5</i> and <i>HLA-DRB1</i> was fully matched. Therefore, NGS-based HLA typing enables a detailed, high-resolution analysis of the HLA system that can help with the selection of suitable donors. Notably, <i>HLA-DRB1*08:02:01G</i> was significantly associated with myelodysplastic syndrome. Although this result confirms the tendency of some alleles to be associated with hematological disorders, this may not be the case in hematologic malignancies. Nonetheless, NGS-based HLA typing data for <i>HLA-DP</i>, <i>HLA-DQ</i>, and <i>HLA-DRB3</i>/4/5 are still warranted for a better understanding of the corresponding locus.https://www.mdpi.com/2075-4418/12/5/1074human leukocyte antigenHLA typingnext generation sequencinghematologic malignancy |
| spellingShingle | Namsoo Kim Sinyoung Kim Jong Rak Choi Younhee Park A Single-Center Experience on HLA Typing with 11 Loci Next Generation Sequencing in Korean Patients with Hematologic Disease Diagnostics human leukocyte antigen HLA typing next generation sequencing hematologic malignancy |
| title | A Single-Center Experience on HLA Typing with 11 Loci Next Generation Sequencing in Korean Patients with Hematologic Disease |
| title_full | A Single-Center Experience on HLA Typing with 11 Loci Next Generation Sequencing in Korean Patients with Hematologic Disease |
| title_fullStr | A Single-Center Experience on HLA Typing with 11 Loci Next Generation Sequencing in Korean Patients with Hematologic Disease |
| title_full_unstemmed | A Single-Center Experience on HLA Typing with 11 Loci Next Generation Sequencing in Korean Patients with Hematologic Disease |
| title_short | A Single-Center Experience on HLA Typing with 11 Loci Next Generation Sequencing in Korean Patients with Hematologic Disease |
| title_sort | single center experience on hla typing with 11 loci next generation sequencing in korean patients with hematologic disease |
| topic | human leukocyte antigen HLA typing next generation sequencing hematologic malignancy |
| url | https://www.mdpi.com/2075-4418/12/5/1074 |
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