Detection of <i>Theileria orientalis</i> Genotypes from Cattle in Kyrgyzstan
The ikeda and chitose genotypes of <i>Theileria orientalis</i>, which for many years were thought to be benign, cause a disease that results in significant economic losses in the cattle industry. This study was carried out in order to determine the genotypes of <i>T. orientalis<...
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MDPI AG
2022-10-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/10/1185 |
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| author | Sezayi Ozubek Mehmet Can Ulucesme Veli Yılgor Cirak Munir Aktas |
| author_facet | Sezayi Ozubek Mehmet Can Ulucesme Veli Yılgor Cirak Munir Aktas |
| author_sort | Sezayi Ozubek |
| collection | DOAJ |
| description | The ikeda and chitose genotypes of <i>Theileria orientalis</i>, which for many years were thought to be benign, cause a disease that results in significant economic losses in the cattle industry. This study was carried out in order to determine the genotypes of <i>T. orientalis</i> in cattle in Kyrgyzstan, and 149 archived DNA samples known to be <i>T. orientalis</i> were analyzed by the PCR amplification of the major piroplasm surface protein (MPSP) gene region. Single-Strand Conformation Polymorphism (SSCP) analysis was performed to uncover the nucleotide changes in the archived DNA samples, and 15 samples showing different band profiles were subjected to sequence analysis. As a result of the sequence analysis, it was seen that the samples belonged to the buffeli and chitose A genotypes. In order to identify mixed genotypes, PCR was performed using primers specific for these genotypes, and buffeli (type 3), chitose (type 1) and buffeli+chitose were found to be positive in 26.2%, 2% and 71.8% of samples, respectively. As a result of this study, we showed the presence of buffeli (type 3) and chitose (type 1) genotypes of <i>T. orientalis</i> in cattle in Kyrgyzstan. Comprehensive epidemiological studies are needed to understand the clinical infections caused by the pathogenic chitose A and to determine the geographical distribution and different genotypes of <i>T. orientalis</i>. |
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| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T19:37:34Z |
| publishDate | 2022-10-01 |
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| series | Pathogens |
| spelling | doaj.art-6eb2792306ae4fa6a2ef2432c54a5cdf2023-11-24T01:49:27ZengMDPI AGPathogens2076-08172022-10-011110118510.3390/pathogens11101185Detection of <i>Theileria orientalis</i> Genotypes from Cattle in KyrgyzstanSezayi Ozubek0Mehmet Can Ulucesme1Veli Yılgor Cirak2Munir Aktas3Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, Elazig 23119, TurkiyeDepartment of Parasitology, Faculty of Veterinary Medicine, University of Firat, Elazig 23119, TurkiyeDepartment of Parasitology, Faculty of Veterinary Medicine, Bursa Uludag University, Bursa 23119, TurkiyeDepartment of Parasitology, Faculty of Veterinary Medicine, University of Firat, Elazig 23119, TurkiyeThe ikeda and chitose genotypes of <i>Theileria orientalis</i>, which for many years were thought to be benign, cause a disease that results in significant economic losses in the cattle industry. This study was carried out in order to determine the genotypes of <i>T. orientalis</i> in cattle in Kyrgyzstan, and 149 archived DNA samples known to be <i>T. orientalis</i> were analyzed by the PCR amplification of the major piroplasm surface protein (MPSP) gene region. Single-Strand Conformation Polymorphism (SSCP) analysis was performed to uncover the nucleotide changes in the archived DNA samples, and 15 samples showing different band profiles were subjected to sequence analysis. As a result of the sequence analysis, it was seen that the samples belonged to the buffeli and chitose A genotypes. In order to identify mixed genotypes, PCR was performed using primers specific for these genotypes, and buffeli (type 3), chitose (type 1) and buffeli+chitose were found to be positive in 26.2%, 2% and 71.8% of samples, respectively. As a result of this study, we showed the presence of buffeli (type 3) and chitose (type 1) genotypes of <i>T. orientalis</i> in cattle in Kyrgyzstan. Comprehensive epidemiological studies are needed to understand the clinical infections caused by the pathogenic chitose A and to determine the geographical distribution and different genotypes of <i>T. orientalis</i>.https://www.mdpi.com/2076-0817/11/10/1185<i>Theileria</i> <i>orientalis</i>genotypecattlePCR |
| spellingShingle | Sezayi Ozubek Mehmet Can Ulucesme Veli Yılgor Cirak Munir Aktas Detection of <i>Theileria orientalis</i> Genotypes from Cattle in Kyrgyzstan Pathogens <i>Theileria</i> <i>orientalis</i> genotype cattle PCR |
| title | Detection of <i>Theileria orientalis</i> Genotypes from Cattle in Kyrgyzstan |
| title_full | Detection of <i>Theileria orientalis</i> Genotypes from Cattle in Kyrgyzstan |
| title_fullStr | Detection of <i>Theileria orientalis</i> Genotypes from Cattle in Kyrgyzstan |
| title_full_unstemmed | Detection of <i>Theileria orientalis</i> Genotypes from Cattle in Kyrgyzstan |
| title_short | Detection of <i>Theileria orientalis</i> Genotypes from Cattle in Kyrgyzstan |
| title_sort | detection of i theileria orientalis i genotypes from cattle in kyrgyzstan |
| topic | <i>Theileria</i> <i>orientalis</i> genotype cattle PCR |
| url | https://www.mdpi.com/2076-0817/11/10/1185 |
| work_keys_str_mv | AT sezayiozubek detectionofitheileriaorientalisigenotypesfromcattleinkyrgyzstan AT mehmetcanulucesme detectionofitheileriaorientalisigenotypesfromcattleinkyrgyzstan AT veliyılgorcirak detectionofitheileriaorientalisigenotypesfromcattleinkyrgyzstan AT muniraktas detectionofitheileriaorientalisigenotypesfromcattleinkyrgyzstan |