Genome-wide identification and expression analysis of 3-ketoacyl-CoA synthase gene family in rice (Oryza sativa L.) under cadmium stress
3-Ketoacyl-CoA synthase (KCS) is the key rate-limiting enzyme for the synthesis of very long-chain fatty acids (VLCFAs) in plants, which determines the carbon chain length of VLCFAs. However, a comprehensive study of KCSs in Oryza sativa has not been reported yet. In this study, we identified 22 OsK...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1222288/full |
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| author | Lingwei Yang Junchao Fang Jingxin Wang Suozhen Hui Liang Zhou Bo Xu Yujuan Chen Yuanyuan Zhang Changkai Lai Guiai Jiao Zhonghua Sheng Xiangjin Wei Gaoneng Shao Lihong Xie Ling Wang Ying Chen Fengli Zhao Shikai Hu Peisong Hu Shaoqing Tang |
| author_facet | Lingwei Yang Junchao Fang Jingxin Wang Suozhen Hui Liang Zhou Bo Xu Yujuan Chen Yuanyuan Zhang Changkai Lai Guiai Jiao Zhonghua Sheng Xiangjin Wei Gaoneng Shao Lihong Xie Ling Wang Ying Chen Fengli Zhao Shikai Hu Peisong Hu Shaoqing Tang |
| author_sort | Lingwei Yang |
| collection | DOAJ |
| description | 3-Ketoacyl-CoA synthase (KCS) is the key rate-limiting enzyme for the synthesis of very long-chain fatty acids (VLCFAs) in plants, which determines the carbon chain length of VLCFAs. However, a comprehensive study of KCSs in Oryza sativa has not been reported yet. In this study, we identified 22 OsKCS genes in rice, which are unevenly distributed on nine chromosomes. The OsKCS gene family is divided into six subclasses. Many cis-acting elements related to plant growth, light, hormone, and stress response were enriched in the promoters of OsKCS genes. Gene duplication played a crucial role in the expansion of the OsKCS gene family and underwent a strong purifying selection. Quantitative Real-time polymerase chain reaction (qRT-PCR) results revealed that most KCS genes are constitutively expressed. We also revealed that KCS genes responded differently to exogenous cadmium stress in japonica and indica background, and the KCS genes with higher expression in leaves and seeds may have functions under cadmium stress. This study provides a basis for further understanding the functions of KCS genes and the biosynthesis of VLCFA in rice. |
| first_indexed | 2024-03-12T22:06:05Z |
| format | Article |
| id | doaj.art-6eb55ebe3f3645e4a0c1f5218910e6b7 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-12T22:06:05Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-6eb55ebe3f3645e4a0c1f5218910e6b72023-07-24T12:02:51ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-07-011410.3389/fpls.2023.12222881222288Genome-wide identification and expression analysis of 3-ketoacyl-CoA synthase gene family in rice (Oryza sativa L.) under cadmium stressLingwei YangJunchao FangJingxin WangSuozhen HuiLiang ZhouBo XuYujuan ChenYuanyuan ZhangChangkai LaiGuiai JiaoZhonghua ShengXiangjin WeiGaoneng ShaoLihong XieLing WangYing ChenFengli ZhaoShikai HuPeisong HuShaoqing Tang3-Ketoacyl-CoA synthase (KCS) is the key rate-limiting enzyme for the synthesis of very long-chain fatty acids (VLCFAs) in plants, which determines the carbon chain length of VLCFAs. However, a comprehensive study of KCSs in Oryza sativa has not been reported yet. In this study, we identified 22 OsKCS genes in rice, which are unevenly distributed on nine chromosomes. The OsKCS gene family is divided into six subclasses. Many cis-acting elements related to plant growth, light, hormone, and stress response were enriched in the promoters of OsKCS genes. Gene duplication played a crucial role in the expansion of the OsKCS gene family and underwent a strong purifying selection. Quantitative Real-time polymerase chain reaction (qRT-PCR) results revealed that most KCS genes are constitutively expressed. We also revealed that KCS genes responded differently to exogenous cadmium stress in japonica and indica background, and the KCS genes with higher expression in leaves and seeds may have functions under cadmium stress. This study provides a basis for further understanding the functions of KCS genes and the biosynthesis of VLCFA in rice.https://www.frontiersin.org/articles/10.3389/fpls.2023.1222288/fullricevery long-chain fatty acidsβ-ketoacyl-CoA synthasegene familycadmium stress |
| spellingShingle | Lingwei Yang Junchao Fang Jingxin Wang Suozhen Hui Liang Zhou Bo Xu Yujuan Chen Yuanyuan Zhang Changkai Lai Guiai Jiao Zhonghua Sheng Xiangjin Wei Gaoneng Shao Lihong Xie Ling Wang Ying Chen Fengli Zhao Shikai Hu Peisong Hu Shaoqing Tang Genome-wide identification and expression analysis of 3-ketoacyl-CoA synthase gene family in rice (Oryza sativa L.) under cadmium stress Frontiers in Plant Science rice very long-chain fatty acids β-ketoacyl-CoA synthase gene family cadmium stress |
| title | Genome-wide identification and expression analysis of 3-ketoacyl-CoA synthase gene family in rice (Oryza sativa L.) under cadmium stress |
| title_full | Genome-wide identification and expression analysis of 3-ketoacyl-CoA synthase gene family in rice (Oryza sativa L.) under cadmium stress |
| title_fullStr | Genome-wide identification and expression analysis of 3-ketoacyl-CoA synthase gene family in rice (Oryza sativa L.) under cadmium stress |
| title_full_unstemmed | Genome-wide identification and expression analysis of 3-ketoacyl-CoA synthase gene family in rice (Oryza sativa L.) under cadmium stress |
| title_short | Genome-wide identification and expression analysis of 3-ketoacyl-CoA synthase gene family in rice (Oryza sativa L.) under cadmium stress |
| title_sort | genome wide identification and expression analysis of 3 ketoacyl coa synthase gene family in rice oryza sativa l under cadmium stress |
| topic | rice very long-chain fatty acids β-ketoacyl-CoA synthase gene family cadmium stress |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1222288/full |
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