Effects of Additional Xylanase on Saccharification and Ethanol Fermentation of Ammonia-Pretreated Corn Stover and Rice Straw
Synergistic effect of cellulase and hemicellulase (xylanase) was evaluated because lignocellulosic material is a heterogeneous complex of cellulose and hemicellulose. Various effects of HTec2 addition on enzymatic saccharification and fermentation were evaluated using two different substrates such a...
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MDPI AG
2020-09-01
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| Series: | Energies |
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| Online Access: | https://www.mdpi.com/1996-1073/13/17/4574 |
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| author | Seung Hyeon Park Thi Thu Huong Pham Tae Hyun Kim |
| author_facet | Seung Hyeon Park Thi Thu Huong Pham Tae Hyun Kim |
| author_sort | Seung Hyeon Park |
| collection | DOAJ |
| description | Synergistic effect of cellulase and hemicellulase (xylanase) was evaluated because lignocellulosic material is a heterogeneous complex of cellulose and hemicellulose. Various effects of HTec2 addition on enzymatic saccharification and fermentation were evaluated using two different substrates such as corn stover and rice straw. Corn stover and rice straw were pretreated by the LMAA (low-moisture anhydrous ammonia) method at the preselected same conditions (90 °C, 120 h, moisture content = 50%, NH<sub>3</sub> loading = 0.1 g NH<sub>3</sub>/g). It was observed that the enzymatic saccharification yield of pretreated corn stover (76.4% for glucan digestibility) was higher than that of pretreated rice straw (70.9% for glucan) using CTec2 cellulase without HTec2 addition. Glucan digestibility of pretreated corn stover was significantly increased from 76.4% to 91.1% when the HTec2/CTec2 (<i>v</i>/<i>v</i>) increased from 0 to 10. However, it was interesting that the ethanol production was decreased from 89.9% to 76.3% for SSF and 118.0% to 87.9% for SSCF at higher HTec2/CTec2. As the glucan loading increased from 2.0% to 7.0%, the ethanol yields of both SSF and SSCF were decreased from 96.3% to 88.9% and from 116.6% to 92.4%, respectively. In addition, the smallest inoculum size (optical density of 0.25) resulted in the highest ethanol production (20.5 g/L). |
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| id | doaj.art-6eb6d84726634118bc1834ba8e6838e2 |
| institution | Directory Open Access Journal |
| issn | 1996-1073 |
| language | English |
| last_indexed | 2024-03-10T16:34:54Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Energies |
| spelling | doaj.art-6eb6d84726634118bc1834ba8e6838e22023-11-20T12:29:54ZengMDPI AGEnergies1996-10732020-09-011317457410.3390/en13174574Effects of Additional Xylanase on Saccharification and Ethanol Fermentation of Ammonia-Pretreated Corn Stover and Rice StrawSeung Hyeon Park0Thi Thu Huong Pham1Tae Hyun Kim2Department of Materials Science and Chemical Engineering, Hanyang University, Ansan, Gyeonggi-do 15588, KoreaFaculty of Technology, Van Lang University, Ho Chi Minh City 70000, VietnamDepartment of Materials Science and Chemical Engineering, Hanyang University, Ansan, Gyeonggi-do 15588, KoreaSynergistic effect of cellulase and hemicellulase (xylanase) was evaluated because lignocellulosic material is a heterogeneous complex of cellulose and hemicellulose. Various effects of HTec2 addition on enzymatic saccharification and fermentation were evaluated using two different substrates such as corn stover and rice straw. Corn stover and rice straw were pretreated by the LMAA (low-moisture anhydrous ammonia) method at the preselected same conditions (90 °C, 120 h, moisture content = 50%, NH<sub>3</sub> loading = 0.1 g NH<sub>3</sub>/g). It was observed that the enzymatic saccharification yield of pretreated corn stover (76.4% for glucan digestibility) was higher than that of pretreated rice straw (70.9% for glucan) using CTec2 cellulase without HTec2 addition. Glucan digestibility of pretreated corn stover was significantly increased from 76.4% to 91.1% when the HTec2/CTec2 (<i>v</i>/<i>v</i>) increased from 0 to 10. However, it was interesting that the ethanol production was decreased from 89.9% to 76.3% for SSF and 118.0% to 87.9% for SSCF at higher HTec2/CTec2. As the glucan loading increased from 2.0% to 7.0%, the ethanol yields of both SSF and SSCF were decreased from 96.3% to 88.9% and from 116.6% to 92.4%, respectively. In addition, the smallest inoculum size (optical density of 0.25) resulted in the highest ethanol production (20.5 g/L).https://www.mdpi.com/1996-1073/13/17/4574pretreatmentinoculum sizehigh-solid fermentationenzymatic saccharificationsimultaneous |
| spellingShingle | Seung Hyeon Park Thi Thu Huong Pham Tae Hyun Kim Effects of Additional Xylanase on Saccharification and Ethanol Fermentation of Ammonia-Pretreated Corn Stover and Rice Straw Energies pretreatment inoculum size high-solid fermentation enzymatic saccharification simultaneous |
| title | Effects of Additional Xylanase on Saccharification and Ethanol Fermentation of Ammonia-Pretreated Corn Stover and Rice Straw |
| title_full | Effects of Additional Xylanase on Saccharification and Ethanol Fermentation of Ammonia-Pretreated Corn Stover and Rice Straw |
| title_fullStr | Effects of Additional Xylanase on Saccharification and Ethanol Fermentation of Ammonia-Pretreated Corn Stover and Rice Straw |
| title_full_unstemmed | Effects of Additional Xylanase on Saccharification and Ethanol Fermentation of Ammonia-Pretreated Corn Stover and Rice Straw |
| title_short | Effects of Additional Xylanase on Saccharification and Ethanol Fermentation of Ammonia-Pretreated Corn Stover and Rice Straw |
| title_sort | effects of additional xylanase on saccharification and ethanol fermentation of ammonia pretreated corn stover and rice straw |
| topic | pretreatment inoculum size high-solid fermentation enzymatic saccharification simultaneous |
| url | https://www.mdpi.com/1996-1073/13/17/4574 |
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