Development of an Improved Method of Sample Extraction and Quantitation of Multi-Mycotoxin in Feed by LC-MS/MS
A multi-mycotoxin chromatographic method was developed and validated for the simultaneous quantitation of aflatoxins (AFB1, AFB2, AFG1 and AFG2), ochratoxin A (OTA), zearalenone (ZON), deoxynivalenol (DON), nivalenol (NIV), diacetoxyscirpenol (DAS), fumonisins (FB1, FB2 and FB3), T-2 toxin (T-2) and...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/12/7/462 |
| _version_ | 1797562022731186176 |
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| author | Bahar Nakhjavan Nighat Sami Ahmed Maryam Khosravifard |
| author_facet | Bahar Nakhjavan Nighat Sami Ahmed Maryam Khosravifard |
| author_sort | Bahar Nakhjavan |
| collection | DOAJ |
| description | A multi-mycotoxin chromatographic method was developed and validated for the simultaneous quantitation of aflatoxins (AFB1, AFB2, AFG1 and AFG2), ochratoxin A (OTA), zearalenone (ZON), deoxynivalenol (DON), nivalenol (NIV), diacetoxyscirpenol (DAS), fumonisins (FB1, FB2 and FB3), T-2 toxin (T-2) and HT-2 toxin (HT-2) in feed. The three most popular sample preparation techniques for determination of mycotoxins have been evaluated, and the method with highest recoveries was selected and optimized. This modified QuEChERS (quick, easy, cheap, effective, rugged and safe) approach was based on the extraction with acetonitrile, salting-out and cleanup with lipid removal. A reconstitution process in methanol/water was used to improve the MS responses and then the extracts were analyzed by LC-MS/MS. In this method, the recovery range is 70–100% for DON, DAS, FB1, FB2, FB3, HT-2, T-2, OTA, ZON, AFG1, AFG2, AFB1 and AFB2 and 55% for NIV in the spike range of 2–80 µg/kg. Method robustness was determined with acceptable z-scores in proficiency tests and validation experiments. |
| first_indexed | 2024-03-10T18:22:46Z |
| format | Article |
| id | doaj.art-6ebc8adca5334119ae2d7b9c9ec6acf7 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-10T18:22:46Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-6ebc8adca5334119ae2d7b9c9ec6acf72023-11-20T07:16:10ZengMDPI AGToxins2072-66512020-07-0112746210.3390/toxins12070462Development of an Improved Method of Sample Extraction and Quantitation of Multi-Mycotoxin in Feed by LC-MS/MSBahar Nakhjavan0Nighat Sami Ahmed1Maryam Khosravifard2Center for Analytical Chemistry, California Department of Food and Agriculture, Sacramento, CA 95832, USACenter for Analytical Chemistry, California Department of Food and Agriculture, Sacramento, CA 95832, USACenter for Analytical Chemistry, California Department of Food and Agriculture, Sacramento, CA 95832, USAA multi-mycotoxin chromatographic method was developed and validated for the simultaneous quantitation of aflatoxins (AFB1, AFB2, AFG1 and AFG2), ochratoxin A (OTA), zearalenone (ZON), deoxynivalenol (DON), nivalenol (NIV), diacetoxyscirpenol (DAS), fumonisins (FB1, FB2 and FB3), T-2 toxin (T-2) and HT-2 toxin (HT-2) in feed. The three most popular sample preparation techniques for determination of mycotoxins have been evaluated, and the method with highest recoveries was selected and optimized. This modified QuEChERS (quick, easy, cheap, effective, rugged and safe) approach was based on the extraction with acetonitrile, salting-out and cleanup with lipid removal. A reconstitution process in methanol/water was used to improve the MS responses and then the extracts were analyzed by LC-MS/MS. In this method, the recovery range is 70–100% for DON, DAS, FB1, FB2, FB3, HT-2, T-2, OTA, ZON, AFG1, AFG2, AFB1 and AFB2 and 55% for NIV in the spike range of 2–80 µg/kg. Method robustness was determined with acceptable z-scores in proficiency tests and validation experiments.https://www.mdpi.com/2072-6651/12/7/462mycotoxinsfeedmodified QuEChERSLC-MS/MS |
| spellingShingle | Bahar Nakhjavan Nighat Sami Ahmed Maryam Khosravifard Development of an Improved Method of Sample Extraction and Quantitation of Multi-Mycotoxin in Feed by LC-MS/MS Toxins mycotoxins feed modified QuEChERS LC-MS/MS |
| title | Development of an Improved Method of Sample Extraction and Quantitation of Multi-Mycotoxin in Feed by LC-MS/MS |
| title_full | Development of an Improved Method of Sample Extraction and Quantitation of Multi-Mycotoxin in Feed by LC-MS/MS |
| title_fullStr | Development of an Improved Method of Sample Extraction and Quantitation of Multi-Mycotoxin in Feed by LC-MS/MS |
| title_full_unstemmed | Development of an Improved Method of Sample Extraction and Quantitation of Multi-Mycotoxin in Feed by LC-MS/MS |
| title_short | Development of an Improved Method of Sample Extraction and Quantitation of Multi-Mycotoxin in Feed by LC-MS/MS |
| title_sort | development of an improved method of sample extraction and quantitation of multi mycotoxin in feed by lc ms ms |
| topic | mycotoxins feed modified QuEChERS LC-MS/MS |
| url | https://www.mdpi.com/2072-6651/12/7/462 |
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