Basal protein synthesis rates differ between vastus lateralis and rectus abdominis muscle
Abstract Background In vivo muscle protein synthesis rates are typically assessed by measuring the incorporation rate of stable isotope labelled amino acids in skeletal muscle tissue collected from vastus lateralis muscle. It remains to be established whether muscle protein synthesis rates in the va...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-06-01
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| Series: | Journal of Cachexia, Sarcopenia and Muscle |
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| Online Access: | https://doi.org/10.1002/jcsm.12701 |
| _version_ | 1827280433403920384 |
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| author | Joey S.J. Smeets Astrid M.H. Horstman David P.J. vanDijk Astrid G.M. vanBoxtel Joost F. terWoorst Steven W.M. Olde Damink Olaf E.M.G. Schijns Luc J.C. vanLoon |
| author_facet | Joey S.J. Smeets Astrid M.H. Horstman David P.J. vanDijk Astrid G.M. vanBoxtel Joost F. terWoorst Steven W.M. Olde Damink Olaf E.M.G. Schijns Luc J.C. vanLoon |
| author_sort | Joey S.J. Smeets |
| collection | DOAJ |
| description | Abstract Background In vivo muscle protein synthesis rates are typically assessed by measuring the incorporation rate of stable isotope labelled amino acids in skeletal muscle tissue collected from vastus lateralis muscle. It remains to be established whether muscle protein synthesis rates in the vastus lateralis are representative of muscle protein synthesis rates of other muscle groups. We hypothesized that post‐absorptive muscle protein synthesis rates differ between vastus lateralis and rectus abdominis, pectoralis major, or temporalis muscle in vivo in humans. Methods Twenty‐four patients (62 ± 3 years, 42% female), scheduled to undergo surgery, participated in this study and underwent primed continuous intravenous infusions with l‐[ring‐13C6]‐phenylalanine. During the surgical procedures, serum samples were collected, and muscle tissue was obtained from the vastus lateralis as well as from the rectus abdominis, pectoralis major, or temporalis muscle. Fractional mixed muscle protein synthesis rates (%/h) were assessed by measuring the incorporation of l‐[ring‐13C6]‐phenylalanine into muscle tissue protein. Results Serum l‐[ring‐13C6]‐phenylalanine enrichments did not change throughout the infusion period. Post‐absorptive muscle protein synthesis rates calculated based upon serum l‐[ring‐13C6]‐phenylalanine enrichments did not differ between vastus lateralis and rectus abdominis (0.032 ± 0.004 vs. 0.038 ± 0.003%/h), vastus lateralis and pectoralis major, (0.025 ± 0.003 vs. 0.022 ± 0.005%/h) or vastus lateralis and temporalis (0.047 ± 0.005 vs. 0.043 ± 0.005%/h) muscle, respectively (P > 0.05). When fractional muscle protein synthesis rates were calculated based upon tissue‐free l‐[ring‐13C6]‐phenylalanine enrichments as the preferred precursor pool, muscle protein synthesis rates were significantly higher in rectus abdominis (0.089 ± 0.008%/h) compared with vastus lateralis (0.054 ± 0.005%/h) muscle (P < 0.01). No differences were observed between fractional muscle protein synthesis rates in vastus lateralis and pectoralis major (0.046 ± 0.003 vs. 0.041 ± 0.008%/h) or vastus lateralis and temporalis (0.073 ± 0.008 vs. 0.083 ± 0.011%/h) muscle, respectively. Conclusions Post‐absorptive muscle protein synthesis rates are higher in rectus abdominis when compared with vastus lateralis muscle. Post‐absorptive muscle protein synthesis rates do not differ between vastus lateralis and pectoralis major or temporalis muscle. Protein synthesis rates in muscle tissue samples obtained during surgery do not necessarily represent a good proxy for appendicular skeletal muscle protein synthesis rates. |
| first_indexed | 2024-04-24T08:36:44Z |
| format | Article |
| id | doaj.art-6ebcb87151744c93951ad454362aa953 |
| institution | Directory Open Access Journal |
| issn | 2190-5991 2190-6009 |
| language | English |
| last_indexed | 2024-04-24T08:36:44Z |
| publishDate | 2021-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Cachexia, Sarcopenia and Muscle |
| spelling | doaj.art-6ebcb87151744c93951ad454362aa9532024-04-16T16:28:49ZengWileyJournal of Cachexia, Sarcopenia and Muscle2190-59912190-60092021-06-0112376977810.1002/jcsm.12701Basal protein synthesis rates differ between vastus lateralis and rectus abdominis muscleJoey S.J. Smeets0Astrid M.H. Horstman1David P.J. vanDijk2Astrid G.M. vanBoxtel3Joost F. terWoorst4Steven W.M. Olde Damink5Olaf E.M.G. Schijns6Luc J.C. vanLoon7Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism Maastricht University Medical Centre+ Maastricht The NetherlandsDepartment of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism Maastricht University Medical Centre+ Maastricht The NetherlandsDepartment of Surgery, NUTRIM School of Nutrition and Translational Research in Metabolism Maastricht University Medical Centre+ Maastricht The NetherlandsDepartment of Cardiothoracic Surgery Catharina Hospital Eindhoven The NetherlandsDepartment of Cardiothoracic Surgery Catharina Hospital Eindhoven The NetherlandsDepartment of Surgery, NUTRIM School of Nutrition and Translational Research in Metabolism Maastricht University Medical Centre+ Maastricht The NetherlandsDepartment of Neurosurgery Maastricht University Medical Centre+ Maastricht The NetherlandsDepartment of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism Maastricht University Medical Centre+ Maastricht The NetherlandsAbstract Background In vivo muscle protein synthesis rates are typically assessed by measuring the incorporation rate of stable isotope labelled amino acids in skeletal muscle tissue collected from vastus lateralis muscle. It remains to be established whether muscle protein synthesis rates in the vastus lateralis are representative of muscle protein synthesis rates of other muscle groups. We hypothesized that post‐absorptive muscle protein synthesis rates differ between vastus lateralis and rectus abdominis, pectoralis major, or temporalis muscle in vivo in humans. Methods Twenty‐four patients (62 ± 3 years, 42% female), scheduled to undergo surgery, participated in this study and underwent primed continuous intravenous infusions with l‐[ring‐13C6]‐phenylalanine. During the surgical procedures, serum samples were collected, and muscle tissue was obtained from the vastus lateralis as well as from the rectus abdominis, pectoralis major, or temporalis muscle. Fractional mixed muscle protein synthesis rates (%/h) were assessed by measuring the incorporation of l‐[ring‐13C6]‐phenylalanine into muscle tissue protein. Results Serum l‐[ring‐13C6]‐phenylalanine enrichments did not change throughout the infusion period. Post‐absorptive muscle protein synthesis rates calculated based upon serum l‐[ring‐13C6]‐phenylalanine enrichments did not differ between vastus lateralis and rectus abdominis (0.032 ± 0.004 vs. 0.038 ± 0.003%/h), vastus lateralis and pectoralis major, (0.025 ± 0.003 vs. 0.022 ± 0.005%/h) or vastus lateralis and temporalis (0.047 ± 0.005 vs. 0.043 ± 0.005%/h) muscle, respectively (P > 0.05). When fractional muscle protein synthesis rates were calculated based upon tissue‐free l‐[ring‐13C6]‐phenylalanine enrichments as the preferred precursor pool, muscle protein synthesis rates were significantly higher in rectus abdominis (0.089 ± 0.008%/h) compared with vastus lateralis (0.054 ± 0.005%/h) muscle (P < 0.01). No differences were observed between fractional muscle protein synthesis rates in vastus lateralis and pectoralis major (0.046 ± 0.003 vs. 0.041 ± 0.008%/h) or vastus lateralis and temporalis (0.073 ± 0.008 vs. 0.083 ± 0.011%/h) muscle, respectively. Conclusions Post‐absorptive muscle protein synthesis rates are higher in rectus abdominis when compared with vastus lateralis muscle. Post‐absorptive muscle protein synthesis rates do not differ between vastus lateralis and pectoralis major or temporalis muscle. Protein synthesis rates in muscle tissue samples obtained during surgery do not necessarily represent a good proxy for appendicular skeletal muscle protein synthesis rates.https://doi.org/10.1002/jcsm.12701Protein turnoverStable isotope methodologyCancer surgeryRectus abdominisPectoralis majorTemporalis |
| spellingShingle | Joey S.J. Smeets Astrid M.H. Horstman David P.J. vanDijk Astrid G.M. vanBoxtel Joost F. terWoorst Steven W.M. Olde Damink Olaf E.M.G. Schijns Luc J.C. vanLoon Basal protein synthesis rates differ between vastus lateralis and rectus abdominis muscle Journal of Cachexia, Sarcopenia and Muscle Protein turnover Stable isotope methodology Cancer surgery Rectus abdominis Pectoralis major Temporalis |
| title | Basal protein synthesis rates differ between vastus lateralis and rectus abdominis muscle |
| title_full | Basal protein synthesis rates differ between vastus lateralis and rectus abdominis muscle |
| title_fullStr | Basal protein synthesis rates differ between vastus lateralis and rectus abdominis muscle |
| title_full_unstemmed | Basal protein synthesis rates differ between vastus lateralis and rectus abdominis muscle |
| title_short | Basal protein synthesis rates differ between vastus lateralis and rectus abdominis muscle |
| title_sort | basal protein synthesis rates differ between vastus lateralis and rectus abdominis muscle |
| topic | Protein turnover Stable isotope methodology Cancer surgery Rectus abdominis Pectoralis major Temporalis |
| url | https://doi.org/10.1002/jcsm.12701 |
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