Gravitational effects on fibroblasts’ function in relation to wound healing
Abstract The spaceflight environment imposes risks for maintaining a healthy skin function as the observed delayed wound healing can contribute to increased risks of infection. To counteract delayed wound healing in space, a better understanding of the fibroblasts’ reaction to altered gravity levels...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-06-01
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| Series: | npj Microgravity |
| Online Access: | https://doi.org/10.1038/s41526-023-00286-z |
| _version_ | 1797425476654858240 |
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| author | Wilhelmina E. Radstake Kiran Gautam Silvana Miranda Cynthia Van Rompay Randy Vermeesen Kevin Tabury Mieke Verslegers Alan Dowson Jeffrey Gorissen Jack J. W. A. van Loon Nigel D. L. Savage Sarah Baatout Bjorn Baselet |
| author_facet | Wilhelmina E. Radstake Kiran Gautam Silvana Miranda Cynthia Van Rompay Randy Vermeesen Kevin Tabury Mieke Verslegers Alan Dowson Jeffrey Gorissen Jack J. W. A. van Loon Nigel D. L. Savage Sarah Baatout Bjorn Baselet |
| author_sort | Wilhelmina E. Radstake |
| collection | DOAJ |
| description | Abstract The spaceflight environment imposes risks for maintaining a healthy skin function as the observed delayed wound healing can contribute to increased risks of infection. To counteract delayed wound healing in space, a better understanding of the fibroblasts’ reaction to altered gravity levels is needed. In this paper, we describe experiments that were carried out at the Large Diameter Centrifuge located in ESA-ESTEC as part of the ESA Academy 2021 Spin Your Thesis! Campaign. We exposed dermal fibroblasts to a set of altered gravity levels, including transitions between simulated microgravity and hypergravity. The addition of the stress hormone cortisol to the cell culture medium was done to account for possible interaction effects of gravity and cortisol exposure. Results show a main impact of cortisol on the secretion of pro-inflammatory cytokines as well as extracellular matrix proteins. Altered gravity mostly induced a delay in cellular migration and changes in mechanosensitive cell structures. Furthermore, 20 × g hypergravity transitions induced changes in nuclear morphology. These findings provide insights into the effect of gravity transitions on the fibroblasts’ function related to wound healing, which may be useful for the development of countermeasures. |
| first_indexed | 2024-03-09T08:15:44Z |
| format | Article |
| id | doaj.art-6ebf281ea3424629a1a9f401beedf4e0 |
| institution | Directory Open Access Journal |
| issn | 2373-8065 |
| language | English |
| last_indexed | 2024-03-09T08:15:44Z |
| publishDate | 2023-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Microgravity |
| spelling | doaj.art-6ebf281ea3424629a1a9f401beedf4e02023-12-02T22:16:15ZengNature Portfolionpj Microgravity2373-80652023-06-019111110.1038/s41526-023-00286-zGravitational effects on fibroblasts’ function in relation to wound healingWilhelmina E. Radstake0Kiran Gautam1Silvana Miranda2Cynthia Van Rompay3Randy Vermeesen4Kevin Tabury5Mieke Verslegers6Alan Dowson7Jeffrey Gorissen8Jack J. W. A. van Loon9Nigel D. L. Savage10Sarah Baatout11Bjorn Baselet12Radiobiology Unit, SCK CEN, Belgian Nuclear Research CentreRadiobiology Unit, SCK CEN, Belgian Nuclear Research CentreRadiobiology Unit, SCK CEN, Belgian Nuclear Research CentreRadiobiology Unit, SCK CEN, Belgian Nuclear Research CentreRadiobiology Unit, SCK CEN, Belgian Nuclear Research CentreRadiobiology Unit, SCK CEN, Belgian Nuclear Research CentreRadiobiology Unit, SCK CEN, Belgian Nuclear Research CentreSerco Nederland for the European Space Agency (ESA), European Space Research and Technology Centre (ESTEC), TEC-MMGESA/ESTECESA/ESTECHE Space Operations for the European Space Agency, ESA/ESTECRadiobiology Unit, SCK CEN, Belgian Nuclear Research CentreRadiobiology Unit, SCK CEN, Belgian Nuclear Research CentreAbstract The spaceflight environment imposes risks for maintaining a healthy skin function as the observed delayed wound healing can contribute to increased risks of infection. To counteract delayed wound healing in space, a better understanding of the fibroblasts’ reaction to altered gravity levels is needed. In this paper, we describe experiments that were carried out at the Large Diameter Centrifuge located in ESA-ESTEC as part of the ESA Academy 2021 Spin Your Thesis! Campaign. We exposed dermal fibroblasts to a set of altered gravity levels, including transitions between simulated microgravity and hypergravity. The addition of the stress hormone cortisol to the cell culture medium was done to account for possible interaction effects of gravity and cortisol exposure. Results show a main impact of cortisol on the secretion of pro-inflammatory cytokines as well as extracellular matrix proteins. Altered gravity mostly induced a delay in cellular migration and changes in mechanosensitive cell structures. Furthermore, 20 × g hypergravity transitions induced changes in nuclear morphology. These findings provide insights into the effect of gravity transitions on the fibroblasts’ function related to wound healing, which may be useful for the development of countermeasures.https://doi.org/10.1038/s41526-023-00286-z |
| spellingShingle | Wilhelmina E. Radstake Kiran Gautam Silvana Miranda Cynthia Van Rompay Randy Vermeesen Kevin Tabury Mieke Verslegers Alan Dowson Jeffrey Gorissen Jack J. W. A. van Loon Nigel D. L. Savage Sarah Baatout Bjorn Baselet Gravitational effects on fibroblasts’ function in relation to wound healing npj Microgravity |
| title | Gravitational effects on fibroblasts’ function in relation to wound healing |
| title_full | Gravitational effects on fibroblasts’ function in relation to wound healing |
| title_fullStr | Gravitational effects on fibroblasts’ function in relation to wound healing |
| title_full_unstemmed | Gravitational effects on fibroblasts’ function in relation to wound healing |
| title_short | Gravitational effects on fibroblasts’ function in relation to wound healing |
| title_sort | gravitational effects on fibroblasts function in relation to wound healing |
| url | https://doi.org/10.1038/s41526-023-00286-z |
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