The Dose- and Time-Dependent Cytotoxic Effect of Graphene Nanoplatelets: In Vitro and In Vivo Study
Graphene-based nanomaterials received attention from scientists due to their unique properties: they are highly conductive, mechanically resistant and elastic. These materials can be used in different sectors of society from electronic energy storage in industry to biomedical applications. This stud...
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MDPI AG
2022-06-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/12/12/1978 |
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| author | Hana Bavorova Tereza Svadlakova Zdenek Fiala Rishikaysh Pisal Jaroslav Mokry |
| author_facet | Hana Bavorova Tereza Svadlakova Zdenek Fiala Rishikaysh Pisal Jaroslav Mokry |
| author_sort | Hana Bavorova |
| collection | DOAJ |
| description | Graphene-based nanomaterials received attention from scientists due to their unique properties: they are highly conductive, mechanically resistant and elastic. These materials can be used in different sectors of society from electronic energy storage in industry to biomedical applications. This study evaluates the influence of graphene nanoplatelets in vitro and in vivo. The toxicological influence of graphene nanoplatelets (GPs) was analyzed by cytotoxic methods, the change of cell proliferation was assessed in real-time, and the effect of GPs on a living organism was evaluated in an animal model using histopathological examination. We analyzed two types of GP administration: intratracheal and peroral. We found dose- and time-dependent cytotoxic effects of GPs in vitro; the concentration above 50 μg/mL increased the cytotoxicity significantly. The real-time analysis confirmed these data; the cells exposed to a high concentration of GPs for a longer time period resulted in a decrease in cell index which indicated lower cell viability. Histopathological examination revealed thickened alveolar septa and accumulation of GPs in the endocardium after intratracheal exposure. Peroral administration did not reveal any morphological changes. This study showed the dose- and time-dependent cytotoxic potential of graphene nanoplatelets in in vitro and in vivo models. |
| first_indexed | 2024-03-09T22:53:15Z |
| format | Article |
| id | doaj.art-6ec625d2eff1417f860961d87123706e |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T22:53:15Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-6ec625d2eff1417f860961d87123706e2023-11-23T18:15:29ZengMDPI AGNanomaterials2079-49912022-06-011212197810.3390/nano12121978The Dose- and Time-Dependent Cytotoxic Effect of Graphene Nanoplatelets: In Vitro and In Vivo StudyHana Bavorova0Tereza Svadlakova1Zdenek Fiala2Rishikaysh Pisal3Jaroslav Mokry4Department of Histology and Embryology, Faculty of Medicine in Hradec Kralove, Charles University, 50003 Hradec Kralove, Czech RepublicInstitute of Clinical Immunology and Allergology, University Hospital Hradec Kralove and Faculty of Medicine in Hradec Kralove, Charles University, 50005 Hradec Kralove, Czech RepublicInstitute of Preventive Medicine, Faculty of Medicine in Hradec Kralove, Charles University, 50003 Hradec Kralove, Czech RepublicDepartment of Histology and Embryology, Faculty of Medicine in Hradec Kralove, Charles University, 50003 Hradec Kralove, Czech RepublicDepartment of Histology and Embryology, Faculty of Medicine in Hradec Kralove, Charles University, 50003 Hradec Kralove, Czech RepublicGraphene-based nanomaterials received attention from scientists due to their unique properties: they are highly conductive, mechanically resistant and elastic. These materials can be used in different sectors of society from electronic energy storage in industry to biomedical applications. This study evaluates the influence of graphene nanoplatelets in vitro and in vivo. The toxicological influence of graphene nanoplatelets (GPs) was analyzed by cytotoxic methods, the change of cell proliferation was assessed in real-time, and the effect of GPs on a living organism was evaluated in an animal model using histopathological examination. We analyzed two types of GP administration: intratracheal and peroral. We found dose- and time-dependent cytotoxic effects of GPs in vitro; the concentration above 50 μg/mL increased the cytotoxicity significantly. The real-time analysis confirmed these data; the cells exposed to a high concentration of GPs for a longer time period resulted in a decrease in cell index which indicated lower cell viability. Histopathological examination revealed thickened alveolar septa and accumulation of GPs in the endocardium after intratracheal exposure. Peroral administration did not reveal any morphological changes. This study showed the dose- and time-dependent cytotoxic potential of graphene nanoplatelets in in vitro and in vivo models.https://www.mdpi.com/2079-4991/12/12/1978graphene nanoplateletscytotoxicitynanomaterialsPAECsC57Bl/6 mice |
| spellingShingle | Hana Bavorova Tereza Svadlakova Zdenek Fiala Rishikaysh Pisal Jaroslav Mokry The Dose- and Time-Dependent Cytotoxic Effect of Graphene Nanoplatelets: In Vitro and In Vivo Study Nanomaterials graphene nanoplatelets cytotoxicity nanomaterials PAECs C57Bl/6 mice |
| title | The Dose- and Time-Dependent Cytotoxic Effect of Graphene Nanoplatelets: In Vitro and In Vivo Study |
| title_full | The Dose- and Time-Dependent Cytotoxic Effect of Graphene Nanoplatelets: In Vitro and In Vivo Study |
| title_fullStr | The Dose- and Time-Dependent Cytotoxic Effect of Graphene Nanoplatelets: In Vitro and In Vivo Study |
| title_full_unstemmed | The Dose- and Time-Dependent Cytotoxic Effect of Graphene Nanoplatelets: In Vitro and In Vivo Study |
| title_short | The Dose- and Time-Dependent Cytotoxic Effect of Graphene Nanoplatelets: In Vitro and In Vivo Study |
| title_sort | dose and time dependent cytotoxic effect of graphene nanoplatelets in vitro and in vivo study |
| topic | graphene nanoplatelets cytotoxicity nanomaterials PAECs C57Bl/6 mice |
| url | https://www.mdpi.com/2079-4991/12/12/1978 |
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