Upcycling waste tilapia (oreochromis niloticus) scales through a decellularization process for extracellular matrix extraction
Tilapia scales, typically regarded as low-value biological waste, possess valuable organic components such as collagen and inorganic components such as hydroxyapatite. Converting these wastes into functional biomaterials like decellularized extracellular matrix (dECM) contributes to long-term fish w...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
IOP Publishing
2024-01-01
|
| Series: | Materials Research Express |
| Subjects: | |
| Online Access: | https://doi.org/10.1088/2053-1591/ad1c01 |
| _version_ | 1797336110989312000 |
|---|---|
| author | Fernan M Arellano Ronald Bual Zesreal Cain Bantilan Johnel Alimasag Jemwel Aron Lean Baclayon Michael John Nisperos Kit Dominick Don Valle Princess Grace Ducao Gladine Lumancas Job Jr Pague Marionilo Jr Labares Hernando Bacosa |
| author_facet | Fernan M Arellano Ronald Bual Zesreal Cain Bantilan Johnel Alimasag Jemwel Aron Lean Baclayon Michael John Nisperos Kit Dominick Don Valle Princess Grace Ducao Gladine Lumancas Job Jr Pague Marionilo Jr Labares Hernando Bacosa |
| author_sort | Fernan M Arellano |
| collection | DOAJ |
| description | Tilapia scales, typically regarded as low-value biological waste, possess valuable organic components such as collagen and inorganic components such as hydroxyapatite. Converting these wastes into functional biomaterials like decellularized extracellular matrix (dECM) contributes to long-term fish waste management to achieve sustainable fish consumption and promote a circular economy by creating a product with a high market value. Triton X-100 (TX-100) and Sodium-dodecyl sulfate (SDS) at varying concentrations (0.1%, 0.5%, and 1%) are utilized to extract the extracellular matrix. The study aims to evaluate the effectiveness of these agents in decellularizing the scales and producing a suitable dECM scaffold. Histological analysis using H&E staining revealed a significant decrease in cellular components in the decellularized samples. This was supported by the dsDNA quantification results showing the highest removal rate of 96% in the samples treated with 1% SDS. Attenuated Total Reflectance-Fourier-transform infrared (ATR-FTIR) spectra showed the amide peaks (Amide A, B, I, II, and III) indicating the presence of type I collagen. The confirmation of type I collagen was further supported by the analysis of SDS-PAGE images, which displayed the presence of α 1 and α 2 chains, β -dimer, and the γ -band. Among the dECM, samples treated with 0.1% TX-100 exhibited the highest protein content, averaging 593.33 ± 17.78 μ g mg ^−1 while 1% SDS showed the lowest protein content averaging 61.33 ± 24.03 μ g mg ^−1 . All dECM samples demonstrated protein denaturation temperatures ranging from 70–75 °C. This study presents baseline data of the single chemical decellularization treatment method utilized to extract ECM from tilapia scales and its resulting dECM. Further research is recommended to assess the biocompatibility and cytotoxicity of the produced dECM and explore its potential applications. |
| first_indexed | 2024-03-08T08:49:21Z |
| format | Article |
| id | doaj.art-6ec7a32979b843a3ab491c47a1ccc710 |
| institution | Directory Open Access Journal |
| issn | 2053-1591 |
| language | English |
| last_indexed | 2024-03-08T08:49:21Z |
| publishDate | 2024-01-01 |
| publisher | IOP Publishing |
| record_format | Article |
| series | Materials Research Express |
| spelling | doaj.art-6ec7a32979b843a3ab491c47a1ccc7102024-02-01T12:24:56ZengIOP PublishingMaterials Research Express2053-15912024-01-0111202510110.1088/2053-1591/ad1c01Upcycling waste tilapia (oreochromis niloticus) scales through a decellularization process for extracellular matrix extractionFernan M Arellano0https://orcid.org/0000-0003-3001-3024Ronald Bual1Zesreal Cain Bantilan2Johnel Alimasag3Jemwel Aron4https://orcid.org/0009-0000-0245-2455Lean Baclayon5Michael John Nisperos6Kit Dominick Don Valle7Princess Grace Ducao8Gladine Lumancas9https://orcid.org/0009-0008-6718-7101Job Jr Pague10Marionilo Jr Labares11Hernando Bacosa12Environmental Science Graduate Program-Department of Biological Sciences, MSU-Iligan Institute of Technology , Iligan City, 9200, PhilippinesCenter for Sustainable Polymers, MSU-Iligan Institute of Technology , Iligan City, 9200, Philippines; Department of Chemical Engineering and Technology, College of Engineering, MSU-Iligan Institute of Technology , Iligan City, 9200, PhilippinesCenter for Sustainable Polymers, MSU-Iligan Institute of Technology , Iligan City, 9200, PhilippinesCenter for Sustainable Polymers, MSU-Iligan Institute of Technology , Iligan City, 9200, PhilippinesEnvironmental Science Graduate Program-Department of Biological Sciences, MSU-Iligan Institute of Technology , Iligan City, 9200, PhilippinesEnvironmental Science Graduate Program-Department of Biological Sciences, MSU-Iligan Institute of Technology , Iligan City, 9200, PhilippinesEnvironmental Science Graduate Program-Department of Biological Sciences, MSU-Iligan Institute of Technology , Iligan City, 9200, PhilippinesCenter for Sustainable Polymers, MSU-Iligan Institute of Technology , Iligan City, 9200, PhilippinesCenter for Sustainable Polymers, MSU-Iligan Institute of Technology , Iligan City, 9200, PhilippinesEnvironmental Science Graduate Program-Department of Biological Sciences, MSU-Iligan Institute of Technology , Iligan City, 9200, PhilippinesCenter for Sustainable Polymers, MSU-Iligan Institute of Technology , Iligan City, 9200, PhilippinesCenter for Sustainable Polymers, MSU-Iligan Institute of Technology , Iligan City, 9200, PhilippinesEnvironmental Science Graduate Program-Department of Biological Sciences, MSU-Iligan Institute of Technology , Iligan City, 9200, Philippines; Center for Sustainable Polymers, MSU-Iligan Institute of Technology , Iligan City, 9200, PhilippinesTilapia scales, typically regarded as low-value biological waste, possess valuable organic components such as collagen and inorganic components such as hydroxyapatite. Converting these wastes into functional biomaterials like decellularized extracellular matrix (dECM) contributes to long-term fish waste management to achieve sustainable fish consumption and promote a circular economy by creating a product with a high market value. Triton X-100 (TX-100) and Sodium-dodecyl sulfate (SDS) at varying concentrations (0.1%, 0.5%, and 1%) are utilized to extract the extracellular matrix. The study aims to evaluate the effectiveness of these agents in decellularizing the scales and producing a suitable dECM scaffold. Histological analysis using H&E staining revealed a significant decrease in cellular components in the decellularized samples. This was supported by the dsDNA quantification results showing the highest removal rate of 96% in the samples treated with 1% SDS. Attenuated Total Reflectance-Fourier-transform infrared (ATR-FTIR) spectra showed the amide peaks (Amide A, B, I, II, and III) indicating the presence of type I collagen. The confirmation of type I collagen was further supported by the analysis of SDS-PAGE images, which displayed the presence of α 1 and α 2 chains, β -dimer, and the γ -band. Among the dECM, samples treated with 0.1% TX-100 exhibited the highest protein content, averaging 593.33 ± 17.78 μ g mg ^−1 while 1% SDS showed the lowest protein content averaging 61.33 ± 24.03 μ g mg ^−1 . All dECM samples demonstrated protein denaturation temperatures ranging from 70–75 °C. This study presents baseline data of the single chemical decellularization treatment method utilized to extract ECM from tilapia scales and its resulting dECM. Further research is recommended to assess the biocompatibility and cytotoxicity of the produced dECM and explore its potential applications.https://doi.org/10.1088/2053-1591/ad1c01tilapiascalesdecellularizationextracellular matrixfish wasteupcycling |
| spellingShingle | Fernan M Arellano Ronald Bual Zesreal Cain Bantilan Johnel Alimasag Jemwel Aron Lean Baclayon Michael John Nisperos Kit Dominick Don Valle Princess Grace Ducao Gladine Lumancas Job Jr Pague Marionilo Jr Labares Hernando Bacosa Upcycling waste tilapia (oreochromis niloticus) scales through a decellularization process for extracellular matrix extraction Materials Research Express tilapia scales decellularization extracellular matrix fish waste upcycling |
| title | Upcycling waste tilapia (oreochromis niloticus) scales through a decellularization process for extracellular matrix extraction |
| title_full | Upcycling waste tilapia (oreochromis niloticus) scales through a decellularization process for extracellular matrix extraction |
| title_fullStr | Upcycling waste tilapia (oreochromis niloticus) scales through a decellularization process for extracellular matrix extraction |
| title_full_unstemmed | Upcycling waste tilapia (oreochromis niloticus) scales through a decellularization process for extracellular matrix extraction |
| title_short | Upcycling waste tilapia (oreochromis niloticus) scales through a decellularization process for extracellular matrix extraction |
| title_sort | upcycling waste tilapia oreochromis niloticus scales through a decellularization process for extracellular matrix extraction |
| topic | tilapia scales decellularization extracellular matrix fish waste upcycling |
| url | https://doi.org/10.1088/2053-1591/ad1c01 |
| work_keys_str_mv | AT fernanmarellano upcyclingwastetilapiaoreochromisniloticusscalesthroughadecellularizationprocessforextracellularmatrixextraction AT ronaldbual upcyclingwastetilapiaoreochromisniloticusscalesthroughadecellularizationprocessforextracellularmatrixextraction AT zesrealcainbantilan upcyclingwastetilapiaoreochromisniloticusscalesthroughadecellularizationprocessforextracellularmatrixextraction AT johnelalimasag upcyclingwastetilapiaoreochromisniloticusscalesthroughadecellularizationprocessforextracellularmatrixextraction AT jemwelaron upcyclingwastetilapiaoreochromisniloticusscalesthroughadecellularizationprocessforextracellularmatrixextraction AT leanbaclayon upcyclingwastetilapiaoreochromisniloticusscalesthroughadecellularizationprocessforextracellularmatrixextraction AT michaeljohnnisperos upcyclingwastetilapiaoreochromisniloticusscalesthroughadecellularizationprocessforextracellularmatrixextraction AT kitdominickdonvalle upcyclingwastetilapiaoreochromisniloticusscalesthroughadecellularizationprocessforextracellularmatrixextraction AT princessgraceducao upcyclingwastetilapiaoreochromisniloticusscalesthroughadecellularizationprocessforextracellularmatrixextraction AT gladinelumancas upcyclingwastetilapiaoreochromisniloticusscalesthroughadecellularizationprocessforextracellularmatrixextraction AT jobjrpague upcyclingwastetilapiaoreochromisniloticusscalesthroughadecellularizationprocessforextracellularmatrixextraction AT marionilojrlabares upcyclingwastetilapiaoreochromisniloticusscalesthroughadecellularizationprocessforextracellularmatrixextraction AT hernandobacosa upcyclingwastetilapiaoreochromisniloticusscalesthroughadecellularizationprocessforextracellularmatrixextraction |