Inoculation and co-inoculation of alfalfa seedlings with root growth promoting microorganisms (Piriformospora indica, Glomus intraradices and Sinorhizobium meliloti) affect molecular structures, nutrient profiles and availability of hay for ruminants
Inoculation of alfalfa seedlings with root growth promoting microorganisms under semi-arid climate condition may improve biomass production and nutritive value. The current study aimed to investigate the effect of inoculation of alfalfa seedlings with Piriformospora indica (Pi) and co-inoculating Pi...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2018-03-01
|
| Series: | Animal Nutrition |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405654516302013 |
| _version_ | 1818450228461699072 |
|---|---|
| author | Milad Jafari Mojtaba Yari Mehdi Ghabooli Mozgan Sepehri Ebrahim Ghasemi Arjan Jonker |
| author_facet | Milad Jafari Mojtaba Yari Mehdi Ghabooli Mozgan Sepehri Ebrahim Ghasemi Arjan Jonker |
| author_sort | Milad Jafari |
| collection | DOAJ |
| description | Inoculation of alfalfa seedlings with root growth promoting microorganisms under semi-arid climate condition may improve biomass production and nutritive value. The current study aimed to investigate the effect of inoculation of alfalfa seedlings with Piriformospora indica (Pi) and co-inoculating Pi with Glomus intraradices (Gi + Pi) or Sinorhizobium meliloti (Sm + Pi) on hay yield, chemical composition, molecular structures by Fourier transformed infrared (FTIR) spectroscopy, in situ ruminal degradability and in vitro gas production. Seedlings were grown in experimental pots in a greenhouse until first cut and then transferred outside and cut a further 4 times. Biomass yield was similar across the treatments. Acid detergent fiber (ADF) concentration was higher in Pi than control hay, and ADF decreased further with co-inoculation (P < 0.05). The ether extract (EE) concentration was lower for Pi and Gi + Pi compared with control hay, and control, Pi and Gi + Pi hays had lower EE concentration compared with Sm + Pi (P < 0.05). The FTIR spectroscopic vibration peak height ratio related to proteins (amide 1 + amide 2): total carbohydrate ratio was lower for the inoculation treatments compared with control hay (P < 0.05). In situ ruminal degradability of dry matter (DM) and organic matter were higher for hay of inoculated and co-inoculated seedlings than for control hay (P < 0.05). In conclusion, hay of alfalfa seedlings inoculated and co-inoculated with root growth promoting microorganisms had improved nutritional value compared with hay from non-treated alfalfa seedlings, and co-inoculation was the most effective, however, changes were relatively minor. Keywords: Alfalfa hay, FTIR spectroscopy, Nutrient availability, Piriformospora indica, Ruminal degradability |
| first_indexed | 2024-12-14T20:47:58Z |
| format | Article |
| id | doaj.art-6ecb5ca54a7f4a14aac7041ca6db8016 |
| institution | Directory Open Access Journal |
| issn | 2405-6545 |
| language | English |
| last_indexed | 2024-12-14T20:47:58Z |
| publishDate | 2018-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Animal Nutrition |
| spelling | doaj.art-6ecb5ca54a7f4a14aac7041ca6db80162022-12-21T22:47:57ZengKeAi Communications Co., Ltd.Animal Nutrition2405-65452018-03-01419099Inoculation and co-inoculation of alfalfa seedlings with root growth promoting microorganisms (Piriformospora indica, Glomus intraradices and Sinorhizobium meliloti) affect molecular structures, nutrient profiles and availability of hay for ruminantsMilad Jafari0Mojtaba Yari1Mehdi Ghabooli2Mozgan Sepehri3Ebrahim Ghasemi4Arjan Jonker5Department of Animal Science, College of Agriculture, Malayer University, Malayer 65719-95863, IranDepartment of Animal Science, College of Agriculture, Malayer University, Malayer 65719-95863, Iran; Corresponding author.Department of Agronomy and Plant Breeding, College of Agriculture, Malayer University, Malayer 65719-95863, IranDepartment of Soil Science, College of Agriculture, Shiraz University, Shiraz 71946-85115, IranDepartment of Animal Science, College of Agriculture, Isfahan University of Technology, Isfahan 84156-83111, IranGrasslands Research Centre, AgResearch Ltd., Palmerston North 11008, New ZealandInoculation of alfalfa seedlings with root growth promoting microorganisms under semi-arid climate condition may improve biomass production and nutritive value. The current study aimed to investigate the effect of inoculation of alfalfa seedlings with Piriformospora indica (Pi) and co-inoculating Pi with Glomus intraradices (Gi + Pi) or Sinorhizobium meliloti (Sm + Pi) on hay yield, chemical composition, molecular structures by Fourier transformed infrared (FTIR) spectroscopy, in situ ruminal degradability and in vitro gas production. Seedlings were grown in experimental pots in a greenhouse until first cut and then transferred outside and cut a further 4 times. Biomass yield was similar across the treatments. Acid detergent fiber (ADF) concentration was higher in Pi than control hay, and ADF decreased further with co-inoculation (P < 0.05). The ether extract (EE) concentration was lower for Pi and Gi + Pi compared with control hay, and control, Pi and Gi + Pi hays had lower EE concentration compared with Sm + Pi (P < 0.05). The FTIR spectroscopic vibration peak height ratio related to proteins (amide 1 + amide 2): total carbohydrate ratio was lower for the inoculation treatments compared with control hay (P < 0.05). In situ ruminal degradability of dry matter (DM) and organic matter were higher for hay of inoculated and co-inoculated seedlings than for control hay (P < 0.05). In conclusion, hay of alfalfa seedlings inoculated and co-inoculated with root growth promoting microorganisms had improved nutritional value compared with hay from non-treated alfalfa seedlings, and co-inoculation was the most effective, however, changes were relatively minor. Keywords: Alfalfa hay, FTIR spectroscopy, Nutrient availability, Piriformospora indica, Ruminal degradabilityhttp://www.sciencedirect.com/science/article/pii/S2405654516302013 |
| spellingShingle | Milad Jafari Mojtaba Yari Mehdi Ghabooli Mozgan Sepehri Ebrahim Ghasemi Arjan Jonker Inoculation and co-inoculation of alfalfa seedlings with root growth promoting microorganisms (Piriformospora indica, Glomus intraradices and Sinorhizobium meliloti) affect molecular structures, nutrient profiles and availability of hay for ruminants Animal Nutrition |
| title | Inoculation and co-inoculation of alfalfa seedlings with root growth promoting microorganisms (Piriformospora indica, Glomus intraradices and Sinorhizobium meliloti) affect molecular structures, nutrient profiles and availability of hay for ruminants |
| title_full | Inoculation and co-inoculation of alfalfa seedlings with root growth promoting microorganisms (Piriformospora indica, Glomus intraradices and Sinorhizobium meliloti) affect molecular structures, nutrient profiles and availability of hay for ruminants |
| title_fullStr | Inoculation and co-inoculation of alfalfa seedlings with root growth promoting microorganisms (Piriformospora indica, Glomus intraradices and Sinorhizobium meliloti) affect molecular structures, nutrient profiles and availability of hay for ruminants |
| title_full_unstemmed | Inoculation and co-inoculation of alfalfa seedlings with root growth promoting microorganisms (Piriformospora indica, Glomus intraradices and Sinorhizobium meliloti) affect molecular structures, nutrient profiles and availability of hay for ruminants |
| title_short | Inoculation and co-inoculation of alfalfa seedlings with root growth promoting microorganisms (Piriformospora indica, Glomus intraradices and Sinorhizobium meliloti) affect molecular structures, nutrient profiles and availability of hay for ruminants |
| title_sort | inoculation and co inoculation of alfalfa seedlings with root growth promoting microorganisms piriformospora indica glomus intraradices and sinorhizobium meliloti affect molecular structures nutrient profiles and availability of hay for ruminants |
| url | http://www.sciencedirect.com/science/article/pii/S2405654516302013 |
| work_keys_str_mv | AT miladjafari inoculationandcoinoculationofalfalfaseedlingswithrootgrowthpromotingmicroorganismspiriformosporaindicaglomusintraradicesandsinorhizobiummelilotiaffectmolecularstructuresnutrientprofilesandavailabilityofhayforruminants AT mojtabayari inoculationandcoinoculationofalfalfaseedlingswithrootgrowthpromotingmicroorganismspiriformosporaindicaglomusintraradicesandsinorhizobiummelilotiaffectmolecularstructuresnutrientprofilesandavailabilityofhayforruminants AT mehdighabooli inoculationandcoinoculationofalfalfaseedlingswithrootgrowthpromotingmicroorganismspiriformosporaindicaglomusintraradicesandsinorhizobiummelilotiaffectmolecularstructuresnutrientprofilesandavailabilityofhayforruminants AT mozgansepehri inoculationandcoinoculationofalfalfaseedlingswithrootgrowthpromotingmicroorganismspiriformosporaindicaglomusintraradicesandsinorhizobiummelilotiaffectmolecularstructuresnutrientprofilesandavailabilityofhayforruminants AT ebrahimghasemi inoculationandcoinoculationofalfalfaseedlingswithrootgrowthpromotingmicroorganismspiriformosporaindicaglomusintraradicesandsinorhizobiummelilotiaffectmolecularstructuresnutrientprofilesandavailabilityofhayforruminants AT arjanjonker inoculationandcoinoculationofalfalfaseedlingswithrootgrowthpromotingmicroorganismspiriformosporaindicaglomusintraradicesandsinorhizobiummelilotiaffectmolecularstructuresnutrientprofilesandavailabilityofhayforruminants |