A possible role for integrin signaling in diffuse axonal injury.
Over the past decade, investigators have attempted to establish the pathophysiological mechanisms by which non-penetrating injuries damage the brain. Several studies have implicated either membrane poration or ion channel dysfunction pursuant to neuronal cell death as the primary mechanism of injury...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3142195?pdf=render |
| _version_ | 1828749159715307520 |
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| author | Matthew A Hemphill Borna E Dabiri Sylvain Gabriele Lucas Kerscher Christian Franck Josue A Goss Patrick W Alford Kevin Kit Parker |
| author_facet | Matthew A Hemphill Borna E Dabiri Sylvain Gabriele Lucas Kerscher Christian Franck Josue A Goss Patrick W Alford Kevin Kit Parker |
| author_sort | Matthew A Hemphill |
| collection | DOAJ |
| description | Over the past decade, investigators have attempted to establish the pathophysiological mechanisms by which non-penetrating injuries damage the brain. Several studies have implicated either membrane poration or ion channel dysfunction pursuant to neuronal cell death as the primary mechanism of injury. We hypothesized that traumatic stimulation of integrins may be an important etiological contributor to mild Traumatic Brain Injury. In order to study the effects of forces at the cellular level, we utilized two hierarchical, in vitro systems to mimic traumatic injury to rat cortical neurons: a high velocity stretcher and a magnetic tweezer system. In one system, we controlled focal adhesion formation in neurons cultured on a stretchable substrate loaded with an abrupt, one dimensional strain. With the second system, we used magnetic tweezers to directly simulate the abrupt injury forces endured by a focal adhesion on the neurite. Both systems revealed variations in the rate and nature of neuronal injury as a function of focal adhesion density and direct integrin stimulation without membrane poration. Pharmacological inhibition of calpains did not mitigate the injury yet the inhibition of Rho-kinase immediately after injury reduced axonal injury. These data suggest that integrin-mediated activation of Rho may be a contributor to the diffuse axonal injury reported in mild Traumatic Brain Injury. |
| first_indexed | 2024-12-10T11:48:43Z |
| format | Article |
| id | doaj.art-6ecee150792e4e0ea2aa32258f766617 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T11:48:43Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-6ecee150792e4e0ea2aa32258f7666172022-12-22T01:50:00ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0167e2289910.1371/journal.pone.0022899A possible role for integrin signaling in diffuse axonal injury.Matthew A HemphillBorna E DabiriSylvain GabrieleLucas KerscherChristian FranckJosue A GossPatrick W AlfordKevin Kit ParkerOver the past decade, investigators have attempted to establish the pathophysiological mechanisms by which non-penetrating injuries damage the brain. Several studies have implicated either membrane poration or ion channel dysfunction pursuant to neuronal cell death as the primary mechanism of injury. We hypothesized that traumatic stimulation of integrins may be an important etiological contributor to mild Traumatic Brain Injury. In order to study the effects of forces at the cellular level, we utilized two hierarchical, in vitro systems to mimic traumatic injury to rat cortical neurons: a high velocity stretcher and a magnetic tweezer system. In one system, we controlled focal adhesion formation in neurons cultured on a stretchable substrate loaded with an abrupt, one dimensional strain. With the second system, we used magnetic tweezers to directly simulate the abrupt injury forces endured by a focal adhesion on the neurite. Both systems revealed variations in the rate and nature of neuronal injury as a function of focal adhesion density and direct integrin stimulation without membrane poration. Pharmacological inhibition of calpains did not mitigate the injury yet the inhibition of Rho-kinase immediately after injury reduced axonal injury. These data suggest that integrin-mediated activation of Rho may be a contributor to the diffuse axonal injury reported in mild Traumatic Brain Injury.http://europepmc.org/articles/PMC3142195?pdf=render |
| spellingShingle | Matthew A Hemphill Borna E Dabiri Sylvain Gabriele Lucas Kerscher Christian Franck Josue A Goss Patrick W Alford Kevin Kit Parker A possible role for integrin signaling in diffuse axonal injury. PLoS ONE |
| title | A possible role for integrin signaling in diffuse axonal injury. |
| title_full | A possible role for integrin signaling in diffuse axonal injury. |
| title_fullStr | A possible role for integrin signaling in diffuse axonal injury. |
| title_full_unstemmed | A possible role for integrin signaling in diffuse axonal injury. |
| title_short | A possible role for integrin signaling in diffuse axonal injury. |
| title_sort | possible role for integrin signaling in diffuse axonal injury |
| url | http://europepmc.org/articles/PMC3142195?pdf=render |
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