Karakteristik Gen Sitokrom C Oksidase Sub Unit I (CO1) Lebah Liar Apis cerena (Hymenoptera: apidae) Asal Pulau Hoga Sulawesi Tenggara
The study was conducted to assess the caracteristic of cytochrome C oxidase subunit I (COI) gene on wild honey bee Apis cerena, and detection of barcode sites from these gene. A total fifteen individual A. cerena were collected from Hoga Island, Southeast Sulawesi. Genomic DNA was extracted from tor...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Universitas Udayana
2018-07-01
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| Series: | Jurnal Veteriner |
| Online Access: | https://ojs.unud.ac.id/index.php/jvet/article/view/36362 |
| _version_ | 1828725677094862848 |
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| author | Suriana Suriana Jamili Jamili Parakkasi Parakkasi |
| author_facet | Suriana Suriana Jamili Jamili Parakkasi Parakkasi |
| author_sort | Suriana Suriana |
| collection | DOAJ |
| description | The study was conducted to assess the caracteristic of cytochrome C oxidase subunit I (COI) gene on wild honey bee Apis cerena, and detection of barcode sites from these gene. A total fifteen individual A. cerena were collected from Hoga Island, Southeast Sulawesi. Genomic DNA was extracted from torax, then amplified by PCR method and than sequenced. Sequencing result characterized their nucleotide and amino acid content. The results showed that 595 nucleotides at the 5' end of COI gene of A. cerena very conserved at the most of the sites. Nucleotide dominated by thymine and adenine bases (± 70%). There are 25 barcoding sites for A. cerena. There are two of these sites are diagnostics for A. cerena from of the Hoga Island. From of 595 basepairs nucleotide were prediction encode 198 amino acid, and only eleven percent of the these are varied between species. Amino acid 91th (treonine) and 103th (asparagin) were diagnostics amino acid for A. cerena from Hoga Island, Southeast Sulawesi. Molecular phylogeny reconstructed based on both nucleotide and amino acid sequence placing apis cerena from Hoga Island, Souteast Sulawesi is closely related to Apis cerena from Genbank source. |
| first_indexed | 2024-04-12T13:31:09Z |
| format | Article |
| id | doaj.art-6ed138d5e8f14087b3e9f3edef971c25 |
| institution | Directory Open Access Journal |
| issn | 1411-8327 2477-5665 |
| language | English |
| last_indexed | 2024-04-12T13:31:09Z |
| publishDate | 2018-07-01 |
| publisher | Universitas Udayana |
| record_format | Article |
| series | Jurnal Veteriner |
| spelling | doaj.art-6ed138d5e8f14087b3e9f3edef971c252022-12-22T03:31:11ZengUniversitas UdayanaJurnal Veteriner1411-83272477-56652018-07-0119111612510.19087/jveteriner.2018.19.1.11636362Karakteristik Gen Sitokrom C Oksidase Sub Unit I (CO1) Lebah Liar Apis cerena (Hymenoptera: apidae) Asal Pulau Hoga Sulawesi TenggaraSuriana Suriana0Jamili Jamili1Parakkasi Parakkasi2Jurusan Biologi, Fakultas Matematika Ilmu Pengetahuan Alam, Universitas Halu Oleo, Kampus Hijau Bumi Tri Darma, Anduonohu, Kendari, Sulawesi TenggaraJurusan Biologi, Fakultas Matematika Ilmu Pengetahuan Alam, Universitas Halu Oleo, Kampus Hijau Bumi Tri Darma, Anduonohu, Kendari, Sulawesi TenggaraJurusan Pendidikan Biologi, Fakultas Keguruan Ilmu Pendidikan, UHO. Kampus Hijau Bumi Tri Darma, Anduonohu, Kendari, Sulawesi TenggaraThe study was conducted to assess the caracteristic of cytochrome C oxidase subunit I (COI) gene on wild honey bee Apis cerena, and detection of barcode sites from these gene. A total fifteen individual A. cerena were collected from Hoga Island, Southeast Sulawesi. Genomic DNA was extracted from torax, then amplified by PCR method and than sequenced. Sequencing result characterized their nucleotide and amino acid content. The results showed that 595 nucleotides at the 5' end of COI gene of A. cerena very conserved at the most of the sites. Nucleotide dominated by thymine and adenine bases (± 70%). There are 25 barcoding sites for A. cerena. There are two of these sites are diagnostics for A. cerena from of the Hoga Island. From of 595 basepairs nucleotide were prediction encode 198 amino acid, and only eleven percent of the these are varied between species. Amino acid 91th (treonine) and 103th (asparagin) were diagnostics amino acid for A. cerena from Hoga Island, Southeast Sulawesi. Molecular phylogeny reconstructed based on both nucleotide and amino acid sequence placing apis cerena from Hoga Island, Souteast Sulawesi is closely related to Apis cerena from Genbank source.https://ojs.unud.ac.id/index.php/jvet/article/view/36362 |
| spellingShingle | Suriana Suriana Jamili Jamili Parakkasi Parakkasi Karakteristik Gen Sitokrom C Oksidase Sub Unit I (CO1) Lebah Liar Apis cerena (Hymenoptera: apidae) Asal Pulau Hoga Sulawesi Tenggara Jurnal Veteriner |
| title | Karakteristik Gen Sitokrom C Oksidase Sub Unit I (CO1) Lebah Liar Apis cerena (Hymenoptera: apidae) Asal Pulau Hoga Sulawesi Tenggara |
| title_full | Karakteristik Gen Sitokrom C Oksidase Sub Unit I (CO1) Lebah Liar Apis cerena (Hymenoptera: apidae) Asal Pulau Hoga Sulawesi Tenggara |
| title_fullStr | Karakteristik Gen Sitokrom C Oksidase Sub Unit I (CO1) Lebah Liar Apis cerena (Hymenoptera: apidae) Asal Pulau Hoga Sulawesi Tenggara |
| title_full_unstemmed | Karakteristik Gen Sitokrom C Oksidase Sub Unit I (CO1) Lebah Liar Apis cerena (Hymenoptera: apidae) Asal Pulau Hoga Sulawesi Tenggara |
| title_short | Karakteristik Gen Sitokrom C Oksidase Sub Unit I (CO1) Lebah Liar Apis cerena (Hymenoptera: apidae) Asal Pulau Hoga Sulawesi Tenggara |
| title_sort | karakteristik gen sitokrom c oksidase sub unit i co1 lebah liar apis cerena hymenoptera apidae asal pulau hoga sulawesi tenggara |
| url | https://ojs.unud.ac.id/index.php/jvet/article/view/36362 |
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