Human Serum Albumin Nanoparticles: Synthesis, Optimization and Immobilization with Antituberculosis Drugs
The aim of this study was to create nanoparticles of human serum albumin immobilized with anti-TB drugs (rifampicin, isoniazid) using the desolvation method. Central Composite Design (CCD) was applied to study the effect of albumin, urea, L-cysteine, rifampicin and isoniazid concentration on particl...
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| Format: | Article |
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MDPI AG
2023-06-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/15/13/2774 |
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| author | Aldana Galiyeva Arailym Daribay Tolkyn Zhumagaliyeva Lyazzat Zhaparova Daniyar Sadyrbekov Yerkeblan Tazhbayev |
| author_facet | Aldana Galiyeva Arailym Daribay Tolkyn Zhumagaliyeva Lyazzat Zhaparova Daniyar Sadyrbekov Yerkeblan Tazhbayev |
| author_sort | Aldana Galiyeva |
| collection | DOAJ |
| description | The aim of this study was to create nanoparticles of human serum albumin immobilized with anti-TB drugs (rifampicin, isoniazid) using the desolvation method. Central Composite Design (CCD) was applied to study the effect of albumin, urea, L-cysteine, rifampicin and isoniazid concentration on particle size, polydispersity and loading degree of the drugs. The optimized nanoparticles were spherical in shape with an average particle size of 216.7 ± 3.7 nm and polydispersity of 0.286 ± 4.9. The loading degree of rifampicin and isoniazid in the optimized nanoparticles were 44% and 27%, respectively. The obtained nanoparticles were examined by Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC); the results showed the absence of drug–polymer interactions. The drug release from the polymer matrix was studied using dialysis membranes. |
| first_indexed | 2024-03-11T01:31:21Z |
| format | Article |
| id | doaj.art-6ed559562efa4ef7a522ba43df0e8d4e |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-11T01:31:21Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-6ed559562efa4ef7a522ba43df0e8d4e2023-11-18T17:19:53ZengMDPI AGPolymers2073-43602023-06-011513277410.3390/polym15132774Human Serum Albumin Nanoparticles: Synthesis, Optimization and Immobilization with Antituberculosis DrugsAldana Galiyeva0Arailym Daribay1Tolkyn Zhumagaliyeva2Lyazzat Zhaparova3Daniyar Sadyrbekov4Yerkeblan Tazhbayev5Institute of Chemical Problems, Karagandy University of the Name of Academician E.A. Buketov, Karaganda City 100028, KazakhstanInstitute of Chemical Problems, Karagandy University of the Name of Academician E.A. Buketov, Karaganda City 100028, KazakhstanInstitute of Chemical Problems, Karagandy University of the Name of Academician E.A. Buketov, Karaganda City 100028, KazakhstanInstitute of Chemical Problems, Karagandy University of the Name of Academician E.A. Buketov, Karaganda City 100028, KazakhstanInstitute of Chemical Problems, Karagandy University of the Name of Academician E.A. Buketov, Karaganda City 100028, KazakhstanInstitute of Chemical Problems, Karagandy University of the Name of Academician E.A. Buketov, Karaganda City 100028, KazakhstanThe aim of this study was to create nanoparticles of human serum albumin immobilized with anti-TB drugs (rifampicin, isoniazid) using the desolvation method. Central Composite Design (CCD) was applied to study the effect of albumin, urea, L-cysteine, rifampicin and isoniazid concentration on particle size, polydispersity and loading degree of the drugs. The optimized nanoparticles were spherical in shape with an average particle size of 216.7 ± 3.7 nm and polydispersity of 0.286 ± 4.9. The loading degree of rifampicin and isoniazid in the optimized nanoparticles were 44% and 27%, respectively. The obtained nanoparticles were examined by Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC); the results showed the absence of drug–polymer interactions. The drug release from the polymer matrix was studied using dialysis membranes.https://www.mdpi.com/2073-4360/15/13/2774nanoparticlesalbuminrifampicinisoniazidantituberculosis drugsdesolvation |
| spellingShingle | Aldana Galiyeva Arailym Daribay Tolkyn Zhumagaliyeva Lyazzat Zhaparova Daniyar Sadyrbekov Yerkeblan Tazhbayev Human Serum Albumin Nanoparticles: Synthesis, Optimization and Immobilization with Antituberculosis Drugs Polymers nanoparticles albumin rifampicin isoniazid antituberculosis drugs desolvation |
| title | Human Serum Albumin Nanoparticles: Synthesis, Optimization and Immobilization with Antituberculosis Drugs |
| title_full | Human Serum Albumin Nanoparticles: Synthesis, Optimization and Immobilization with Antituberculosis Drugs |
| title_fullStr | Human Serum Albumin Nanoparticles: Synthesis, Optimization and Immobilization with Antituberculosis Drugs |
| title_full_unstemmed | Human Serum Albumin Nanoparticles: Synthesis, Optimization and Immobilization with Antituberculosis Drugs |
| title_short | Human Serum Albumin Nanoparticles: Synthesis, Optimization and Immobilization with Antituberculosis Drugs |
| title_sort | human serum albumin nanoparticles synthesis optimization and immobilization with antituberculosis drugs |
| topic | nanoparticles albumin rifampicin isoniazid antituberculosis drugs desolvation |
| url | https://www.mdpi.com/2073-4360/15/13/2774 |
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