Efficient genetic transformation method for Eucalyptus genome editing.
Plantation forestry of Eucalyptus urophylla × Eucalyptus grandis supplies high-quality raw material for pulp, paper, wood, and energy and thereby reduces the pressures on native forests and their associated biodiversity. Nevertheless, owing to the heterozygosity of the E. urophylla × E. grandis gene...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0252011 |
| _version_ | 1818983932329197568 |
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| author | Zechen Wang Limei Li Lejun Ouyang |
| author_facet | Zechen Wang Limei Li Lejun Ouyang |
| author_sort | Zechen Wang |
| collection | DOAJ |
| description | Plantation forestry of Eucalyptus urophylla × Eucalyptus grandis supplies high-quality raw material for pulp, paper, wood, and energy and thereby reduces the pressures on native forests and their associated biodiversity. Nevertheless, owing to the heterozygosity of the E. urophylla × E. grandis genetic background, germplasm improvement by crossbreeding tends to be inefficient. As an alternative approach, genetic engineering of Eucalyptus can be used to effectively improve germplasm resources. From a strategic standpoint, increasing the plantation productivity and wood quality by transgenic technology has become increasingly important for forest industry. In this study, we established a fluorescence labelling method using CRISPR/Cas9 technology to obtain positive transformed progenies. The positive transformed progenies were easily obtained from the genetically modified population via fluorescence screening. This system can be used as a plant genome site-specific editing tool and may be useful for improving Eucalyptus genetic resources. |
| first_indexed | 2024-12-20T18:10:58Z |
| format | Article |
| id | doaj.art-6ed65da88d8b49018aa3f013fc902116 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T18:10:58Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-6ed65da88d8b49018aa3f013fc9021162022-12-21T19:30:28ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01165e025201110.1371/journal.pone.0252011Efficient genetic transformation method for Eucalyptus genome editing.Zechen WangLimei LiLejun OuyangPlantation forestry of Eucalyptus urophylla × Eucalyptus grandis supplies high-quality raw material for pulp, paper, wood, and energy and thereby reduces the pressures on native forests and their associated biodiversity. Nevertheless, owing to the heterozygosity of the E. urophylla × E. grandis genetic background, germplasm improvement by crossbreeding tends to be inefficient. As an alternative approach, genetic engineering of Eucalyptus can be used to effectively improve germplasm resources. From a strategic standpoint, increasing the plantation productivity and wood quality by transgenic technology has become increasingly important for forest industry. In this study, we established a fluorescence labelling method using CRISPR/Cas9 technology to obtain positive transformed progenies. The positive transformed progenies were easily obtained from the genetically modified population via fluorescence screening. This system can be used as a plant genome site-specific editing tool and may be useful for improving Eucalyptus genetic resources.https://doi.org/10.1371/journal.pone.0252011 |
| spellingShingle | Zechen Wang Limei Li Lejun Ouyang Efficient genetic transformation method for Eucalyptus genome editing. PLoS ONE |
| title | Efficient genetic transformation method for Eucalyptus genome editing. |
| title_full | Efficient genetic transformation method for Eucalyptus genome editing. |
| title_fullStr | Efficient genetic transformation method for Eucalyptus genome editing. |
| title_full_unstemmed | Efficient genetic transformation method for Eucalyptus genome editing. |
| title_short | Efficient genetic transformation method for Eucalyptus genome editing. |
| title_sort | efficient genetic transformation method for eucalyptus genome editing |
| url | https://doi.org/10.1371/journal.pone.0252011 |
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