Structure and Dynamics of GPCRs in Lipid Membranes: Physical Principles and Experimental Approaches
Over the past decade, the vast amount of information generated through structural and biophysical studies of GPCRs has provided unprecedented mechanistic insight into the complex signalling behaviour of these receptors. With this recent information surge, it has also become increasingly apparent tha...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/20/4729 |
| _version_ | 1797550866090164224 |
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| author | Andrew J. Y. Jones Florian Gabriel Aditi Tandale Daniel Nietlispach |
| author_facet | Andrew J. Y. Jones Florian Gabriel Aditi Tandale Daniel Nietlispach |
| author_sort | Andrew J. Y. Jones |
| collection | DOAJ |
| description | Over the past decade, the vast amount of information generated through structural and biophysical studies of GPCRs has provided unprecedented mechanistic insight into the complex signalling behaviour of these receptors. With this recent information surge, it has also become increasingly apparent that in order to reproduce the various effects that lipids and membranes exert on the biological function for these allosteric receptors, in vitro studies of GPCRs need to be conducted under conditions that adequately approximate the native lipid bilayer environment. In the first part of this review, we assess some of the more general effects that a membrane environment exerts on lipid bilayer-embedded proteins such as GPCRs. This is then followed by the consideration of more specific effects, including stoichiometric interactions with specific lipid subtypes. In the final section, we survey a range of different membrane mimetics that are currently used for in vitro studies, with a focus on NMR applications. |
| first_indexed | 2024-03-10T15:36:29Z |
| format | Article |
| id | doaj.art-6ed8e09b00e94c258fb9f21192245ceb |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T15:36:29Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-6ed8e09b00e94c258fb9f21192245ceb2023-11-20T17:12:18ZengMDPI AGMolecules1420-30492020-10-012520472910.3390/molecules25204729Structure and Dynamics of GPCRs in Lipid Membranes: Physical Principles and Experimental ApproachesAndrew J. Y. Jones0Florian Gabriel1Aditi Tandale2Daniel Nietlispach3Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UKDepartment of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UKDepartment of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UKDepartment of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UKOver the past decade, the vast amount of information generated through structural and biophysical studies of GPCRs has provided unprecedented mechanistic insight into the complex signalling behaviour of these receptors. With this recent information surge, it has also become increasingly apparent that in order to reproduce the various effects that lipids and membranes exert on the biological function for these allosteric receptors, in vitro studies of GPCRs need to be conducted under conditions that adequately approximate the native lipid bilayer environment. In the first part of this review, we assess some of the more general effects that a membrane environment exerts on lipid bilayer-embedded proteins such as GPCRs. This is then followed by the consideration of more specific effects, including stoichiometric interactions with specific lipid subtypes. In the final section, we survey a range of different membrane mimetics that are currently used for in vitro studies, with a focus on NMR applications.https://www.mdpi.com/1420-3049/25/20/4729GPCRlipidsmembrane mimeticsNMR |
| spellingShingle | Andrew J. Y. Jones Florian Gabriel Aditi Tandale Daniel Nietlispach Structure and Dynamics of GPCRs in Lipid Membranes: Physical Principles and Experimental Approaches Molecules GPCR lipids membrane mimetics NMR |
| title | Structure and Dynamics of GPCRs in Lipid Membranes: Physical Principles and Experimental Approaches |
| title_full | Structure and Dynamics of GPCRs in Lipid Membranes: Physical Principles and Experimental Approaches |
| title_fullStr | Structure and Dynamics of GPCRs in Lipid Membranes: Physical Principles and Experimental Approaches |
| title_full_unstemmed | Structure and Dynamics of GPCRs in Lipid Membranes: Physical Principles and Experimental Approaches |
| title_short | Structure and Dynamics of GPCRs in Lipid Membranes: Physical Principles and Experimental Approaches |
| title_sort | structure and dynamics of gpcrs in lipid membranes physical principles and experimental approaches |
| topic | GPCR lipids membrane mimetics NMR |
| url | https://www.mdpi.com/1420-3049/25/20/4729 |
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