Impact of Vitamin D<sub>3</sub> Deficiency on Phosphatidylcholine-/Ethanolamine, Plasmalogen-, Lyso-Phosphatidylcholine-/Ethanolamine, Carnitine- and Triacyl Glyceride-Homeostasis in Neuroblastoma Cells and Murine Brain
Vitamin D<sub>3</sub> hypovitaminosis is associated with several neurological diseases such as Alzheimer’s disease, Parkinson’s disease or multiple sclerosis but also with other diseases such as cancer, diabetes or diseases linked to inflammatory processes. Importantly, in all of these d...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/11/1699 |
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| author | Anna Andrea Lauer Lea Victoria Griebsch Sabrina Melanie Pilz Daniel Janitschke Elena Leoni Theiss Jörg Reichrath Christian Herr Christoph Beisswenger Robert Bals Teresa Giovanna Valencak Dorothea Portius Heike Sabine Grimm Tobias Hartmann Marcus Otto Walter Grimm |
| author_facet | Anna Andrea Lauer Lea Victoria Griebsch Sabrina Melanie Pilz Daniel Janitschke Elena Leoni Theiss Jörg Reichrath Christian Herr Christoph Beisswenger Robert Bals Teresa Giovanna Valencak Dorothea Portius Heike Sabine Grimm Tobias Hartmann Marcus Otto Walter Grimm |
| author_sort | Anna Andrea Lauer |
| collection | DOAJ |
| description | Vitamin D<sub>3</sub> hypovitaminosis is associated with several neurological diseases such as Alzheimer’s disease, Parkinson’s disease or multiple sclerosis but also with other diseases such as cancer, diabetes or diseases linked to inflammatory processes. Importantly, in all of these diseases lipids have at least a disease modifying effect. Besides its well-known property to modulate gene-expression via the VDR-receptor, less is known if vitamin D hypovitaminosis influences lipid homeostasis and if these potential changes contribute to the pathology of the diseases themselves. Therefore, we analyzed mouse brain with a mild vitamin D hypovitaminosis via a targeted shotgun lipidomic approach, including phosphatidylcholine, plasmalogens, lyso-phosphatidylcholine, (acyl-/acetyl-) carnitines and triglycerides. Alterations were compared with neuroblastoma cells cultivated in the presence and with decreased levels of vitamin D. Both in cell culture and in vivo, decreased vitamin D level resulted in changed lipid levels. While triglycerides were decreased, carnitines were increased under vitamin D hypovitaminosis suggesting an impact of vitamin D on energy metabolism. Additionally, lyso-phosphatidylcholines in particular saturated phosphatidylcholine (e.g., PC aa 48:0) and plasmalogen species (e.g., PC ae 42:0) tended to be increased. Our results suggest that vitamin D hypovitaminosis not only may affect gene expression but also may directly influence cellular lipid homeostasis and affect lipid turnover in disease states that are known for vitamin D hypovitaminosis. |
| first_indexed | 2024-03-10T05:40:18Z |
| format | Article |
| id | doaj.art-6ed9758e9c8c4e8cb7de18f91a42be34 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T05:40:18Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-6ed9758e9c8c4e8cb7de18f91a42be342023-11-22T22:35:02ZengMDPI AGBiomolecules2218-273X2021-11-011111169910.3390/biom11111699Impact of Vitamin D<sub>3</sub> Deficiency on Phosphatidylcholine-/Ethanolamine, Plasmalogen-, Lyso-Phosphatidylcholine-/Ethanolamine, Carnitine- and Triacyl Glyceride-Homeostasis in Neuroblastoma Cells and Murine BrainAnna Andrea Lauer0Lea Victoria Griebsch1Sabrina Melanie Pilz2Daniel Janitschke3Elena Leoni Theiss4Jörg Reichrath5Christian Herr6Christoph Beisswenger7Robert Bals8Teresa Giovanna Valencak9Dorothea Portius10Heike Sabine Grimm11Tobias Hartmann12Marcus Otto Walter Grimm13Experimental Neurology, Saarland University, 66421 Homburg, GermanyExperimental Neurology, Saarland University, 66421 Homburg, GermanyExperimental Neurology, Saarland University, 66421 Homburg, GermanyExperimental Neurology, Saarland University, 66421 Homburg, GermanyExperimental Neurology, Saarland University, 66421 Homburg, GermanyDepartment of Dermatology, Saarland University Hospital, 66421 Homburg, GermanyDepartment of Internal Medicine V-Pulmonology, Allergology, Respiratory Intensive Care Medicine, Saarland University Hospital, 66421 Homburg, GermanyDepartment of Internal Medicine V-Pulmonology, Allergology, Respiratory Intensive Care Medicine, Saarland University Hospital, 66421 Homburg, GermanyDepartment of Internal Medicine V-Pulmonology, Allergology, Respiratory Intensive Care Medicine, Saarland University Hospital, 66421 Homburg, GermanyDepartment of Biosciences, Paris Lodron University Salzburg, Hellbrunnerstrasse 34, 5020 Salzburg, GermanyNutrition Therapy and Counseling, Campus Gera, SRH University of Applied Health Science, 07548 Gera, GermanyExperimental Neurology, Saarland University, 66421 Homburg, GermanyDeutsches Institut für Demenzprävention, Saarland University, 66421 Homburg, GermanyExperimental Neurology, Saarland University, 66421 Homburg, GermanyVitamin D<sub>3</sub> hypovitaminosis is associated with several neurological diseases such as Alzheimer’s disease, Parkinson’s disease or multiple sclerosis but also with other diseases such as cancer, diabetes or diseases linked to inflammatory processes. Importantly, in all of these diseases lipids have at least a disease modifying effect. Besides its well-known property to modulate gene-expression via the VDR-receptor, less is known if vitamin D hypovitaminosis influences lipid homeostasis and if these potential changes contribute to the pathology of the diseases themselves. Therefore, we analyzed mouse brain with a mild vitamin D hypovitaminosis via a targeted shotgun lipidomic approach, including phosphatidylcholine, plasmalogens, lyso-phosphatidylcholine, (acyl-/acetyl-) carnitines and triglycerides. Alterations were compared with neuroblastoma cells cultivated in the presence and with decreased levels of vitamin D. Both in cell culture and in vivo, decreased vitamin D level resulted in changed lipid levels. While triglycerides were decreased, carnitines were increased under vitamin D hypovitaminosis suggesting an impact of vitamin D on energy metabolism. Additionally, lyso-phosphatidylcholines in particular saturated phosphatidylcholine (e.g., PC aa 48:0) and plasmalogen species (e.g., PC ae 42:0) tended to be increased. Our results suggest that vitamin D hypovitaminosis not only may affect gene expression but also may directly influence cellular lipid homeostasis and affect lipid turnover in disease states that are known for vitamin D hypovitaminosis.https://www.mdpi.com/2218-273X/11/11/1699vitamin D hypovitaminosisshotgun lipidomicscalcitriolneurodegenerative diseasesphosphatidylcholinephosphatidylcholine plasmalogen |
| spellingShingle | Anna Andrea Lauer Lea Victoria Griebsch Sabrina Melanie Pilz Daniel Janitschke Elena Leoni Theiss Jörg Reichrath Christian Herr Christoph Beisswenger Robert Bals Teresa Giovanna Valencak Dorothea Portius Heike Sabine Grimm Tobias Hartmann Marcus Otto Walter Grimm Impact of Vitamin D<sub>3</sub> Deficiency on Phosphatidylcholine-/Ethanolamine, Plasmalogen-, Lyso-Phosphatidylcholine-/Ethanolamine, Carnitine- and Triacyl Glyceride-Homeostasis in Neuroblastoma Cells and Murine Brain Biomolecules vitamin D hypovitaminosis shotgun lipidomics calcitriol neurodegenerative diseases phosphatidylcholine phosphatidylcholine plasmalogen |
| title | Impact of Vitamin D<sub>3</sub> Deficiency on Phosphatidylcholine-/Ethanolamine, Plasmalogen-, Lyso-Phosphatidylcholine-/Ethanolamine, Carnitine- and Triacyl Glyceride-Homeostasis in Neuroblastoma Cells and Murine Brain |
| title_full | Impact of Vitamin D<sub>3</sub> Deficiency on Phosphatidylcholine-/Ethanolamine, Plasmalogen-, Lyso-Phosphatidylcholine-/Ethanolamine, Carnitine- and Triacyl Glyceride-Homeostasis in Neuroblastoma Cells and Murine Brain |
| title_fullStr | Impact of Vitamin D<sub>3</sub> Deficiency on Phosphatidylcholine-/Ethanolamine, Plasmalogen-, Lyso-Phosphatidylcholine-/Ethanolamine, Carnitine- and Triacyl Glyceride-Homeostasis in Neuroblastoma Cells and Murine Brain |
| title_full_unstemmed | Impact of Vitamin D<sub>3</sub> Deficiency on Phosphatidylcholine-/Ethanolamine, Plasmalogen-, Lyso-Phosphatidylcholine-/Ethanolamine, Carnitine- and Triacyl Glyceride-Homeostasis in Neuroblastoma Cells and Murine Brain |
| title_short | Impact of Vitamin D<sub>3</sub> Deficiency on Phosphatidylcholine-/Ethanolamine, Plasmalogen-, Lyso-Phosphatidylcholine-/Ethanolamine, Carnitine- and Triacyl Glyceride-Homeostasis in Neuroblastoma Cells and Murine Brain |
| title_sort | impact of vitamin d sub 3 sub deficiency on phosphatidylcholine ethanolamine plasmalogen lyso phosphatidylcholine ethanolamine carnitine and triacyl glyceride homeostasis in neuroblastoma cells and murine brain |
| topic | vitamin D hypovitaminosis shotgun lipidomics calcitriol neurodegenerative diseases phosphatidylcholine phosphatidylcholine plasmalogen |
| url | https://www.mdpi.com/2218-273X/11/11/1699 |
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