Research on the high efficient methodology of Establishment of 129S1/SvImJ murine embryonic stem cells(高效建立129S1/SvImJ小鼠胚胎干细胞方法的探讨)
选取超排后129S1/SvImJ品系小鼠的优质囊胚，使用辐照后冻存复苏的小鼠胚胎成纤维细胞作为饲养层，对比高浓度酶短时间消化法和低浓度酶长时间消化法，以0.25% 胰酶-0.04% EDTA高浓度酶短时间消化法高效建立了 4株小鼠胚胎干细胞系，建系率为36.3%.碱性磷酸酶，核型分析，体外分化和体内分化能力以及小鼠胚胎干细胞表面特异性分子标志0ct-4和Nanog的检测等表明，4个ES细胞系均为典型的小鼠胚胎干细胞系.所建立的ES细胞系为大规模培养ES细胞，进行后续的实验建立了理想的材料平台....
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| Format: | Article |
| Language: | zho |
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Zhejiang University Press
2005-09-01
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| Series: | Zhejiang Daxue xuebao. Lixue ban |
| Subjects: | |
| Online Access: | https://doi.org/zjup/1008-9497.2005.32.5.574-578 |
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| author | JIANGZu-yun(姜祖韵) WURong-rong(吴蓉蓉) YUANYi-jun(袁毅君) CHENLiang-biao(陈良标) ZHAOXiao-li(赵小立) LUYong-liang(陆永良) YAOxing(姚行) DAILi-cheng(戴利成) ZHANGMing(张铭) |
| author_facet | JIANGZu-yun(姜祖韵) WURong-rong(吴蓉蓉) YUANYi-jun(袁毅君) CHENLiang-biao(陈良标) ZHAOXiao-li(赵小立) LUYong-liang(陆永良) YAOxing(姚行) DAILi-cheng(戴利成) ZHANGMing(张铭) |
| author_sort | JIANGZu-yun(姜祖韵) |
| collection | DOAJ |
| description | 选取超排后129S1/SvImJ品系小鼠的优质囊胚，使用辐照后冻存复苏的小鼠胚胎成纤维细胞作为饲养层，对比高浓度酶短时间消化法和低浓度酶长时间消化法，以0.25% 胰酶-0.04% EDTA高浓度酶短时间消化法高效建立了 4株小鼠胚胎干细胞系，建系率为36.3%.碱性磷酸酶，核型分析，体外分化和体内分化能力以及小鼠胚胎干细胞表面特异性分子标志0ct-4和Nanog的检测等表明，4个ES细胞系均为典型的小鼠胚胎干细胞系.所建立的ES细胞系为大规模培养ES细胞，进行后续的实验建立了理想的材料平台. |
| first_indexed | 2024-04-24T17:02:41Z |
| format | Article |
| id | doaj.art-6edacfc507ca4671903d892c3e2007df |
| institution | Directory Open Access Journal |
| issn | 1008-9497 |
| language | zho |
| last_indexed | 2024-04-24T17:02:41Z |
| publishDate | 2005-09-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | Zhejiang Daxue xuebao. Lixue ban |
| spelling | doaj.art-6edacfc507ca4671903d892c3e2007df2024-03-29T01:58:22ZzhoZhejiang University PressZhejiang Daxue xuebao. Lixue ban1008-94972005-09-01325574578zjup/1008-9497.2005.32.5.574-578Research on the high efficient methodology of Establishment of 129S1/SvImJ murine embryonic stem cells(高效建立129S1/SvImJ小鼠胚胎干细胞方法的探讨)JIANGZu-yun(姜祖韵)0WURong-rong(吴蓉蓉)1YUANYi-jun(袁毅君)2CHENLiang-biao(陈良标)3ZHAOXiao-li(赵小立)4LUYong-liang(陆永良)5YAOxing(姚行)6DAILi-cheng(戴利成)7ZHANGMing(张铭)8 1.College of Life Science, Zhejiang University, Hangzhou 310012, China( 1.浙江大学生命科学学院，浙江 杭州 310012) 1.College of Life Science, Zhejiang University, Hangzhou 310012, China( 1.浙江大学生命科学学院，浙江 杭州 310012) 2.College of Life Science and Chemistry, Tianshui Normal University, Tianshui 741000, China( 2.天水师范学院生命科学与化学学院，甘肃 天水 741000) 3.Institute of Genetics and Developmental Biology, Chinese Academy of Science, Beijing 100101, China( 3.中国科学院遗传与发育生物学研究所，北京 100101) 1.College of Life Science, Zhejiang University, Hangzhou 310012, China( 1.浙江大学生命科学学院，浙江 杭州 310012) 4.Central Hospital of Huzhou, Huzhou 313000, China( 4.湖州市中心医院，浙江 湖州 313000) 4.Central Hospital of Huzhou, Huzhou 313000, China( 4.湖州市中心医院，浙江 湖州 313000) 4.Central Hospital of Huzhou, Huzhou 313000, China( 4.湖州市中心医院，浙江 湖州 313000) 1.College of Life Science, Zhejiang University, Hangzhou 310012, China( 1.浙江大学生命科学学院，浙江 杭州 310012)选取超排后129S1/SvImJ品系小鼠的优质囊胚，使用辐照后冻存复苏的小鼠胚胎成纤维细胞作为饲养层，对比高浓度酶短时间消化法和低浓度酶长时间消化法，以0.25% 胰酶-0.04% EDTA高浓度酶短时间消化法高效建立了 4株小鼠胚胎干细胞系，建系率为36.3%.碱性磷酸酶，核型分析，体外分化和体内分化能力以及小鼠胚胎干细胞表面特异性分子标志0ct-4和Nanog的检测等表明，4个ES细胞系均为典型的小鼠胚胎干细胞系.所建立的ES细胞系为大规模培养ES细胞，进行后续的实验建立了理想的材料平台.https://doi.org/zjup/1008-9497.2005.32.5.574-578129s1/svimj小鼠囊胚饲养层消化方法胚胎干细胞 |
| spellingShingle | JIANGZu-yun(姜祖韵) WURong-rong(吴蓉蓉) YUANYi-jun(袁毅君) CHENLiang-biao(陈良标) ZHAOXiao-li(赵小立) LUYong-liang(陆永良) YAOxing(姚行) DAILi-cheng(戴利成) ZHANGMing(张铭) Research on the high efficient methodology of Establishment of 129S1/SvImJ murine embryonic stem cells(高效建立129S1/SvImJ小鼠胚胎干细胞方法的探讨) Zhejiang Daxue xuebao. Lixue ban 129s1/svimj小鼠 囊胚 饲养层 消化方法 胚胎干细胞 |
| title | Research on the high efficient methodology of Establishment of 129S1/SvImJ murine embryonic stem cells(高效建立129S1/SvImJ小鼠胚胎干细胞方法的探讨) |
| title_full | Research on the high efficient methodology of Establishment of 129S1/SvImJ murine embryonic stem cells(高效建立129S1/SvImJ小鼠胚胎干细胞方法的探讨) |
| title_fullStr | Research on the high efficient methodology of Establishment of 129S1/SvImJ murine embryonic stem cells(高效建立129S1/SvImJ小鼠胚胎干细胞方法的探讨) |
| title_full_unstemmed | Research on the high efficient methodology of Establishment of 129S1/SvImJ murine embryonic stem cells(高效建立129S1/SvImJ小鼠胚胎干细胞方法的探讨) |
| title_short | Research on the high efficient methodology of Establishment of 129S1/SvImJ murine embryonic stem cells(高效建立129S1/SvImJ小鼠胚胎干细胞方法的探讨) |
| title_sort | research on the high efficient methodology of establishment of 129s1 svimj murine embryonic stem cells 高效建立129s1 svimj小鼠胚胎干细胞方法的探讨 |
| topic | 129s1/svimj小鼠 囊胚 饲养层 消化方法 胚胎干细胞 |
| url | https://doi.org/zjup/1008-9497.2005.32.5.574-578 |
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