Genome-Wide Identification and Characterization of the Trehalose-6-Phosphate Synthetase Gene Family in Chinese Cabbage (<i>Brassica rapa</i>) and <i>Plasmodiophora brassicae</i> during Their Interaction
Trehalose is a nonreducing disaccharide that is widely distributed in various organisms. Trehalose-6-phosphate synthase (TPS) is a critical enzyme responsible for the biosynthesis of trehalose, which serves important functions in growth and development, defense, and stress resistance. Although previ...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/2/929 |
| Summary: | Trehalose is a nonreducing disaccharide that is widely distributed in various organisms. Trehalose-6-phosphate synthase (TPS) is a critical enzyme responsible for the biosynthesis of trehalose, which serves important functions in growth and development, defense, and stress resistance. Although previous studies have found that the clubroot pathogen <i>Plasmodiophora brassicae</i> can lead to the accumulation of trehalose in infected <i>Arabidopsis</i> organs, it has been proposed that much of the accumulated trehalose is derived from the pathogen. At present, there is very little evidence to verify this view. In this study, a comprehensive analysis of the <i>TPS</i> gene family was conducted in <i>Brassica rapa</i> and <i>Plasmodiophora brassicae</i>. A total of 14 <i>Brassica rapa TPS</i> genes (<i>BrTPSs</i>) and 3 <i>P. brassicae TPS</i> genes (<i>PbTPSs</i>) were identified, and the evolutionary characteristics, functional classification, and expression patterns were analyzed. Fourteen <i>BrTPS</i> genes were classified into two distinct classes according to phylogeny and gene structure. Three <i>PbTPSs</i> showed no significant differences in gene structure and protein conserved motifs. However, evolutionary analysis showed that the <i>PbTPS2</i> gene failed to cluster with <i>PbTPS1</i> and <i>PbTPS3</i>. Furthermore, cis-acting elements related to growth and development, defense and stress responsiveness, and hormone responsiveness were predicted in the promoter region of the <i>BrTPS</i> genes. Expression analysis of most <i>BrTPS</i> genes at five stages after <i>P. brassicae</i> interaction found no significant induction. Instead, the expression of the <i>PbTPS</i> genes of <i>P. brassicae</i> was upregulated, which was consistent with the period of trehalose accumulation. This study deepens our understanding of the function and evolution of <i>BrTPSs</i> and <i>PbTPSs</i>. Simultaneously, clarifying the biosynthesis of trehalose in the interaction between <i>Brassica rapa</i> and <i>P. brassicae</i> is also of great significance. |
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| ISSN: | 1661-6596 1422-0067 |