Extreme thermal stability of the antiGFP nanobody – GFP complex
Abstract Objective The green fluorescent protein (GFP) and its derivatives are widely used in biomedical research. The manipulation of GFP-tagged proteins by GFP-specific binders, e.g. single-domain antibodies (nanobodies), is of increasing significance. It is therefore important to better understan...
Main Authors: | Balázs Kakasi, Eszter Gácsi, Hajnalka Jankovics, Ferenc Vonderviszt |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2023-06-01
|
Series: | BMC Research Notes |
Subjects: | |
Online Access: | https://doi.org/10.1186/s13104-023-06382-3 |
Similar Items
-
Optimizing the expression of an anti-Leishmania nanobody ‘ALNb18’ produced free or fused with super folder GFP
by: Mohamed Karam Qassas, et al.
Published: (2021-01-01) -
GFP fusions of Sec-routed extracellular proteins in Staphylococcus aureus reveal surface-associated coagulase in biofilms
by: Dominique C. S. Evans, et al.
Published: (2023-06-01) -
Visualizing the subcellular localization of RHOB-GTP and GTPase-Effector complexes using a split-GFP/nanobody labelling assay
by: Sebastian Castillo, et al.
Published: (2023-12-01) -
Engineering and Characterization of a Fluorescent Native-Like HIV-1 Envelope Glycoprotein Trimer
by: Kwinten Sliepen, et al.
Published: (2015-10-01) -
Tissue-Specific Split sfGFP System for Streamlined Expression of GFP Tagged Proteins in the Caenorhabditis elegans Germline
by: Adam Hefel, et al.
Published: (2019-06-01)