Evaluation of acute myeloid leukemia blast percentage on MethylC-Capture Sequencing results
Abstract Aberrant DNA methylation is often related to the diagnosis, prognosis, and therapeutic response of acute myeloid leukemia (AML); however, relevant studies on the relationship between bone marrow myeloblast percentage and the DNA methylation level in AML have not been reported. We evaluated...
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| Format: | Article |
| Language: | English |
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BMC
2021-03-01
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| Series: | Experimental Hematology & Oncology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40164-021-00219-0 |
| _version_ | 1819078264746934272 |
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| author | Erna Yang Desheng Gong Wei Guan Jieying Li Xuefeng Gao Yonghui Li Li Yu |
| author_facet | Erna Yang Desheng Gong Wei Guan Jieying Li Xuefeng Gao Yonghui Li Li Yu |
| author_sort | Erna Yang |
| collection | DOAJ |
| description | Abstract Aberrant DNA methylation is often related to the diagnosis, prognosis, and therapeutic response of acute myeloid leukemia (AML); however, relevant studies on the relationship between bone marrow myeloblast percentage and the DNA methylation level in AML have not been reported. We evaluated the effects of AML blast percentage on DNA methylation level using the MethylC-capture sequencing (MCC-Seq) approach based on next-generation sequencing (NGS) and found that the methylation level of both genome-wide and promoter regions significantly increased when the percentage of AML blasts reached ≥ 40%, indicating that an accurate DNA methylation level in cancer cells can be obtained when the bone marrow samples of AML patients have more than 40% myeloblasts. |
| first_indexed | 2024-12-21T19:10:20Z |
| format | Article |
| id | doaj.art-6edce62404374ce99dbc92c8e8c007f7 |
| institution | Directory Open Access Journal |
| issn | 2162-3619 |
| language | English |
| last_indexed | 2024-12-21T19:10:20Z |
| publishDate | 2021-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Experimental Hematology & Oncology |
| spelling | doaj.art-6edce62404374ce99dbc92c8e8c007f72022-12-21T18:53:13ZengBMCExperimental Hematology & Oncology2162-36192021-03-011011410.1186/s40164-021-00219-0Evaluation of acute myeloid leukemia blast percentage on MethylC-Capture Sequencing resultsErna Yang0Desheng Gong1Wei Guan2Jieying Li3Xuefeng Gao4Yonghui Li5Li Yu6Department of Hematology and Oncology, International Cancer Center, Shenzhen Key Laboratory of Precision Medicine for Hematological Malignancies, Shenzhen University General Hospital, Shenzhen University Clinical Medical Academy, Shenzhen University Health Science Center, Shenzhen UniversityDepartment of Hematology and Oncology, International Cancer Center, Shenzhen Key Laboratory of Precision Medicine for Hematological Malignancies, Shenzhen University General Hospital, Shenzhen University Clinical Medical Academy, Shenzhen University Health Science Center, Shenzhen UniversityDepartment of Hematology, Chinese PLA General HospitalDepartment of Hematology and Oncology, International Cancer Center, Shenzhen Key Laboratory of Precision Medicine for Hematological Malignancies, Shenzhen University General Hospital, Shenzhen University Clinical Medical Academy, Shenzhen University Health Science Center, Shenzhen UniversityCentrol Laboratory, Shenzhen University General Hospital, Shenzhen University Health Science CenterCentrol Laboratory, Shenzhen University General Hospital, Shenzhen University Health Science CenterDepartment of Hematology and Oncology, International Cancer Center, Shenzhen Key Laboratory of Precision Medicine for Hematological Malignancies, Shenzhen University General Hospital, Shenzhen University Clinical Medical Academy, Shenzhen University Health Science Center, Shenzhen UniversityAbstract Aberrant DNA methylation is often related to the diagnosis, prognosis, and therapeutic response of acute myeloid leukemia (AML); however, relevant studies on the relationship between bone marrow myeloblast percentage and the DNA methylation level in AML have not been reported. We evaluated the effects of AML blast percentage on DNA methylation level using the MethylC-capture sequencing (MCC-Seq) approach based on next-generation sequencing (NGS) and found that the methylation level of both genome-wide and promoter regions significantly increased when the percentage of AML blasts reached ≥ 40%, indicating that an accurate DNA methylation level in cancer cells can be obtained when the bone marrow samples of AML patients have more than 40% myeloblasts.https://doi.org/10.1186/s40164-021-00219-0AMLBlast percentageMethylation statusMCC-Seq |
| spellingShingle | Erna Yang Desheng Gong Wei Guan Jieying Li Xuefeng Gao Yonghui Li Li Yu Evaluation of acute myeloid leukemia blast percentage on MethylC-Capture Sequencing results Experimental Hematology & Oncology AML Blast percentage Methylation status MCC-Seq |
| title | Evaluation of acute myeloid leukemia blast percentage on MethylC-Capture Sequencing results |
| title_full | Evaluation of acute myeloid leukemia blast percentage on MethylC-Capture Sequencing results |
| title_fullStr | Evaluation of acute myeloid leukemia blast percentage on MethylC-Capture Sequencing results |
| title_full_unstemmed | Evaluation of acute myeloid leukemia blast percentage on MethylC-Capture Sequencing results |
| title_short | Evaluation of acute myeloid leukemia blast percentage on MethylC-Capture Sequencing results |
| title_sort | evaluation of acute myeloid leukemia blast percentage on methylc capture sequencing results |
| topic | AML Blast percentage Methylation status MCC-Seq |
| url | https://doi.org/10.1186/s40164-021-00219-0 |
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