Comparative evaluation of various serological tests in the laboratory diagnosis of Brucellosis
Introduction: Brucellosis is a zoonotic disease of public health importance having an impact on animal husbandry and dairy industry. Isolation of bacteria from blood cultures is very low, and diagnosis mainly depends on serology and molecular methods. The aim of this study is to know the impact of v...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2015-01-01
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| Series: | CHRISMED Journal of Health and Research |
| Subjects: | |
| Online Access: | http://www.cjhr.org/article.asp?issn=2348-3334;year=2015;volume=2;issue=2;spage=136;epage=139;aulast=Dias |
| _version_ | 1828376917642838016 |
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| author | Meena Dias Edwin Dias |
| author_facet | Meena Dias Edwin Dias |
| author_sort | Meena Dias |
| collection | DOAJ |
| description | Introduction: Brucellosis is a zoonotic disease of public health importance having an impact on animal husbandry and dairy industry. Isolation of bacteria from blood cultures is very low, and diagnosis mainly depends on serology and molecular methods. The aim of this study is to know the impact of various serological tests on the diagnosis of brucellosis. Materials and Methods: In a prospective study, a total of 180 samples, 90 from pyrexia of unknown origin (PUO) and 90 from high-risk group comprising veterinarians and animal handlers were serologically tested by Rose Bengal plate test, standard tube agglutination (STA) test and enzyme-linked immunosorbent assay (ELISA) and results were analyzed. Results: Out of 90 PUO cases, 28 were positive for brucellosis and in high-risk group, out of 30 veterinarians three were positive and out of 60 animal handlers 14 were positive. Male preponderance was seen. Rose Bengal and STA tests were still efficient methods for brucellosis serodiagnosis. The ELISA was observed to be more efficient in both acute and chronic brucellosis. Conclusion: All the three methods used are efficient methods for detecting Brucella antibody. Rose Bengal card test is the best suited for rapid diagnosis in rural endemic area as STA test is laborious and time-consuming. ELISA is the most sensitive test. |
| first_indexed | 2024-04-14T08:06:13Z |
| format | Article |
| id | doaj.art-6edee7c4d12a4f30a105241eeec3c585 |
| institution | Directory Open Access Journal |
| issn | 2348-3334 2348-506X |
| language | English |
| last_indexed | 2024-04-14T08:06:13Z |
| publishDate | 2015-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | CHRISMED Journal of Health and Research |
| spelling | doaj.art-6edee7c4d12a4f30a105241eeec3c5852022-12-22T02:04:43ZengWolters Kluwer Medknow PublicationsCHRISMED Journal of Health and Research2348-33342348-506X2015-01-012213613910.4103/2348-3334.153258Comparative evaluation of various serological tests in the laboratory diagnosis of BrucellosisMeena DiasEdwin DiasIntroduction: Brucellosis is a zoonotic disease of public health importance having an impact on animal husbandry and dairy industry. Isolation of bacteria from blood cultures is very low, and diagnosis mainly depends on serology and molecular methods. The aim of this study is to know the impact of various serological tests on the diagnosis of brucellosis. Materials and Methods: In a prospective study, a total of 180 samples, 90 from pyrexia of unknown origin (PUO) and 90 from high-risk group comprising veterinarians and animal handlers were serologically tested by Rose Bengal plate test, standard tube agglutination (STA) test and enzyme-linked immunosorbent assay (ELISA) and results were analyzed. Results: Out of 90 PUO cases, 28 were positive for brucellosis and in high-risk group, out of 30 veterinarians three were positive and out of 60 animal handlers 14 were positive. Male preponderance was seen. Rose Bengal and STA tests were still efficient methods for brucellosis serodiagnosis. The ELISA was observed to be more efficient in both acute and chronic brucellosis. Conclusion: All the three methods used are efficient methods for detecting Brucella antibody. Rose Bengal card test is the best suited for rapid diagnosis in rural endemic area as STA test is laborious and time-consuming. ELISA is the most sensitive test.http://www.cjhr.org/article.asp?issn=2348-3334;year=2015;volume=2;issue=2;spage=136;epage=139;aulast=DiasBrucellosisenzyme-linked immunosorbent assayRose Bengal card testserologystandard tube agglutination test |
| spellingShingle | Meena Dias Edwin Dias Comparative evaluation of various serological tests in the laboratory diagnosis of Brucellosis CHRISMED Journal of Health and Research Brucellosis enzyme-linked immunosorbent assay Rose Bengal card test serology standard tube agglutination test |
| title | Comparative evaluation of various serological tests in the laboratory diagnosis of Brucellosis |
| title_full | Comparative evaluation of various serological tests in the laboratory diagnosis of Brucellosis |
| title_fullStr | Comparative evaluation of various serological tests in the laboratory diagnosis of Brucellosis |
| title_full_unstemmed | Comparative evaluation of various serological tests in the laboratory diagnosis of Brucellosis |
| title_short | Comparative evaluation of various serological tests in the laboratory diagnosis of Brucellosis |
| title_sort | comparative evaluation of various serological tests in the laboratory diagnosis of brucellosis |
| topic | Brucellosis enzyme-linked immunosorbent assay Rose Bengal card test serology standard tube agglutination test |
| url | http://www.cjhr.org/article.asp?issn=2348-3334;year=2015;volume=2;issue=2;spage=136;epage=139;aulast=Dias |
| work_keys_str_mv | AT meenadias comparativeevaluationofvariousserologicaltestsinthelaboratorydiagnosisofbrucellosis AT edwindias comparativeevaluationofvariousserologicaltestsinthelaboratorydiagnosisofbrucellosis |