PorA typing of Neisseria meningitidis isolates from Iranian children for vaccine design
Introduction: As the causative agent of meningitis, Neisseria meningitidis has different serogroups. The purpose of this study was to investigate the molecular properties of N. meningitidis strains among Iranian cases. Methods: 450 samples were collected from children under 5 years of age. Detection...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Pasteur Institute of Iran
2018-06-01
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| Series: | Vaccine Research |
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| Online Access: | http://vacres.pasteur.ac.ir/article-1-124-en.html |
| _version_ | 1818302776790220800 |
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| author | P Afrough M Vosogh MR Asadi Karam A Behrouzi G Mardani SD Siadat |
| author_facet | P Afrough M Vosogh MR Asadi Karam A Behrouzi G Mardani SD Siadat |
| author_sort | P Afrough |
| collection | DOAJ |
| description | Introduction: As the causative agent of meningitis, Neisseria meningitidis has different serogroups. The purpose of this study was to investigate the molecular properties of N. meningitidis strains among Iranian cases. Methods: 450 samples were collected from children under 5 years of age. Detection of Neisseria genus was done by phenotypic and genotypic methods. Multiplex PCR was used to identify the serogroups of N. meningitides. The sequencing of variable regions of porA gene was performed for detection of the subserogroups. Results: From 137 (30.44%) Neisseria isolates, 4 isolates (0.88%) belonged to N. meningitidis and 133 isolates (29.55%) belonged to other species. Multiplex PCR results showed that one isolate belonged to serogroup A while 3 belonged to serogroup B. The analysis of amplified VR1 and VR2 variable regions of porA showed 100% identity of the serogroup A strain with strain BZ83N and the serogroup B strains with strain 528 of N. meningitidis . In accordance with other findings in Asia, serogroups A and B were the most prevalent serogroups of N. meningitidis. Sequencing of variable regions of porA could identify the subserogroups of the isolates. Conclusion: sequencing of porA could be a valuable method for identification of N. meningitidis strains to be used in epidemiological studies as well as improved vaccine designs. |
| first_indexed | 2024-12-13T05:44:17Z |
| format | Article |
| id | doaj.art-6ee002c42bb6422ba832477f0edbbbcf |
| institution | Directory Open Access Journal |
| issn | 2383-2819 2423-4923 |
| language | English |
| last_indexed | 2024-12-13T05:44:17Z |
| publishDate | 2018-06-01 |
| publisher | Pasteur Institute of Iran |
| record_format | Article |
| series | Vaccine Research |
| spelling | doaj.art-6ee002c42bb6422ba832477f0edbbbcf2022-12-21T23:57:42ZengPasteur Institute of IranVaccine Research2383-28192423-49232018-06-015146PorA typing of Neisseria meningitidis isolates from Iranian children for vaccine designP Afrough0M Vosogh1MR Asadi Karam2A Behrouzi3G Mardani4SD Siadat5 Department of Mycobacteriology & Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran./ Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran. Department of Mycobacteriology & Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran./ Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran. Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran. Department of Mycobacteriology & Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran./ Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran. Department of Mycobacteriology & Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran. Department of Mycobacteriology & Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran./ Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran. Introduction: As the causative agent of meningitis, Neisseria meningitidis has different serogroups. The purpose of this study was to investigate the molecular properties of N. meningitidis strains among Iranian cases. Methods: 450 samples were collected from children under 5 years of age. Detection of Neisseria genus was done by phenotypic and genotypic methods. Multiplex PCR was used to identify the serogroups of N. meningitides. The sequencing of variable regions of porA gene was performed for detection of the subserogroups. Results: From 137 (30.44%) Neisseria isolates, 4 isolates (0.88%) belonged to N. meningitidis and 133 isolates (29.55%) belonged to other species. Multiplex PCR results showed that one isolate belonged to serogroup A while 3 belonged to serogroup B. The analysis of amplified VR1 and VR2 variable regions of porA showed 100% identity of the serogroup A strain with strain BZ83N and the serogroup B strains with strain 528 of N. meningitidis . In accordance with other findings in Asia, serogroups A and B were the most prevalent serogroups of N. meningitidis. Sequencing of variable regions of porA could identify the subserogroups of the isolates. Conclusion: sequencing of porA could be a valuable method for identification of N. meningitidis strains to be used in epidemiological studies as well as improved vaccine designs.http://vacres.pasteur.ac.ir/article-1-124-en.htmlneisseria meningitidisporasequencingpcrtyping. |
| spellingShingle | P Afrough M Vosogh MR Asadi Karam A Behrouzi G Mardani SD Siadat PorA typing of Neisseria meningitidis isolates from Iranian children for vaccine design Vaccine Research neisseria meningitidis pora sequencing pcr typing. |
| title | PorA typing of Neisseria meningitidis isolates from Iranian children for vaccine design |
| title_full | PorA typing of Neisseria meningitidis isolates from Iranian children for vaccine design |
| title_fullStr | PorA typing of Neisseria meningitidis isolates from Iranian children for vaccine design |
| title_full_unstemmed | PorA typing of Neisseria meningitidis isolates from Iranian children for vaccine design |
| title_short | PorA typing of Neisseria meningitidis isolates from Iranian children for vaccine design |
| title_sort | pora typing of neisseria meningitidis isolates from iranian children for vaccine design |
| topic | neisseria meningitidis pora sequencing pcr typing. |
| url | http://vacres.pasteur.ac.ir/article-1-124-en.html |
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