Porcine Reproductive and Respiratory Syndrome Virus Type 1.3 Lena Triggers Conventional Dendritic Cells 1 Activation and T Helper 1 Immune Response Without Infecting Dendritic Cells
Porcine Reproductive and Respiratory Syndrome virus (PRRSV) is an arterivirus responsible for highly contagious infection and huge economic losses in pig industry. Two species, PRRSV-1 and PRRSV-2 are distinguished, PRRSV-1 being more prevalent in Europe. PRRSV-1 can further be divided in subtypes....
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-10-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2018.02299/full |
| _version_ | 1819015184691232768 |
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| author | Elise Bordet Fany Blanc Mathieu Tiret Elisa Crisci Elisa Crisci Edwige Bouguyon Patricia Renson Patricia Renson Patricia Renson Pauline Maisonnasse Mickael Bourge Jean-Jacques Leplat Elisabetta Giuffra Luc Jouneau Isabelle Schwartz-Cornil Olivier Bourry Olivier Bourry Nicolas Bertho |
| author_facet | Elise Bordet Fany Blanc Mathieu Tiret Elisa Crisci Elisa Crisci Edwige Bouguyon Patricia Renson Patricia Renson Patricia Renson Pauline Maisonnasse Mickael Bourge Jean-Jacques Leplat Elisabetta Giuffra Luc Jouneau Isabelle Schwartz-Cornil Olivier Bourry Olivier Bourry Nicolas Bertho |
| author_sort | Elise Bordet |
| collection | DOAJ |
| description | Porcine Reproductive and Respiratory Syndrome virus (PRRSV) is an arterivirus responsible for highly contagious infection and huge economic losses in pig industry. Two species, PRRSV-1 and PRRSV-2 are distinguished, PRRSV-1 being more prevalent in Europe. PRRSV-1 can further be divided in subtypes. PRRSV-1.3 such as Lena are more pathogenic than PRRSV-1.1 such as Lelystad or Flanders13. PRRSV-1.3 viruses trigger a higher Th1 response than PRRSV-1.1, although the role of the cellular immune response in PRRSV clearance remains ill defined. The pathogenicity as well as the T cell response inductions may be differentially impacted according to the capacity of the virus strain to infect and/or activate DCs. However, the interactions of PRRSV with in vivo-differentiated-DC subtypes such as conventional DC1 (cDC1), cDC2, and monocyte-derived DCs (moDC) have not been thoroughly investigated. Here, DC subpopulations from Lena in vivo infected pigs were analyzed for viral genome detection. This experiment demonstrates that cDC1, cDC2, and moDC are not infected in vivo by Lena. Analysis of DC cytokines production revealed that cDC1 are clearly activated in vivo by Lena. In vitro comparison of 3 Europeans strains revealed no infection of the cDC1 and cDC2 and no or little infection of moDC with Lena, whereas the two PRRSV-1.1 strains infect none of the 3 DC subtypes. In vitro investigation of T helper polarization and cytokines production demonstrate that Lena induces a higher Th1 polarization and IFNγ secretion than FL13 and LV. Altogether, this work suggests an activation of cDC1 by Lena associated with a Th1 immune response polarization. |
| first_indexed | 2024-12-21T02:27:42Z |
| format | Article |
| id | doaj.art-6ee0716dfcb64d949a0f42fa8f82ee22 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-21T02:27:42Z |
| publishDate | 2018-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-6ee0716dfcb64d949a0f42fa8f82ee222022-12-21T19:19:00ZengFrontiers Media S.A.Frontiers in Immunology1664-32242018-10-01910.3389/fimmu.2018.02299382471Porcine Reproductive and Respiratory Syndrome Virus Type 1.3 Lena Triggers Conventional Dendritic Cells 1 Activation and T Helper 1 Immune Response Without Infecting Dendritic CellsElise Bordet0Fany Blanc1Mathieu Tiret2Elisa Crisci3Elisa Crisci4Edwige Bouguyon5Patricia Renson6Patricia Renson7Patricia Renson8Pauline Maisonnasse9Mickael Bourge10Jean-Jacques Leplat11Elisabetta Giuffra12Luc Jouneau13Isabelle Schwartz-Cornil14Olivier Bourry15Olivier Bourry16Nicolas Bertho17Virologie et Immunologie Moléculaire, Institut National de la Recherche Agronomique, Université Paris-Saclay, Jouy-en-Josas, FranceUMR Génétique Animale et Biologie Intégrative, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, FranceUMR Génétique Animale et Biologie Intégrative, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, FranceUMR Génétique Animale et Biologie Intégrative, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, FranceDepartment of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, United StatesVirologie et Immunologie Moléculaire, Institut National de la Recherche Agronomique, Université Paris-Saclay, Jouy-en-Josas, FranceVirologie et Immunologie Porcines, Agence Nationale de Sécurité Sanitaire, Ploufragan, FranceUniversité Bretagne Loire, Rennes, FranceUnion des Groupements de Producteurs de Viande de Bretagne (UGPVB), Rennes, FranceVirologie et Immunologie Moléculaire, Institut National de la Recherche Agronomique, Université Paris-Saclay, Jouy-en-Josas, FranceInstitute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, FranceUMR Génétique Animale et Biologie Intégrative, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, FranceUMR Génétique Animale et Biologie Intégrative, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, FranceVirologie et Immunologie Moléculaire, Institut National de la Recherche Agronomique, Université Paris-Saclay, Jouy-en-Josas, FranceVirologie et Immunologie Moléculaire, Institut National de la Recherche Agronomique, Université Paris-Saclay, Jouy-en-Josas, FranceVirologie et Immunologie Porcines, Agence Nationale de Sécurité Sanitaire, Ploufragan, FranceUniversité Bretagne Loire, Rennes, FranceVirologie et Immunologie Moléculaire, Institut National de la Recherche Agronomique, Université Paris-Saclay, Jouy-en-Josas, FrancePorcine Reproductive and Respiratory Syndrome virus (PRRSV) is an arterivirus responsible for highly contagious infection and huge economic losses in pig industry. Two species, PRRSV-1 and PRRSV-2 are distinguished, PRRSV-1 being more prevalent in Europe. PRRSV-1 can further be divided in subtypes. PRRSV-1.3 such as Lena are more pathogenic than PRRSV-1.1 such as Lelystad or Flanders13. PRRSV-1.3 viruses trigger a higher Th1 response than PRRSV-1.1, although the role of the cellular immune response in PRRSV clearance remains ill defined. The pathogenicity as well as the T cell response inductions may be differentially impacted according to the capacity of the virus strain to infect and/or activate DCs. However, the interactions of PRRSV with in vivo-differentiated-DC subtypes such as conventional DC1 (cDC1), cDC2, and monocyte-derived DCs (moDC) have not been thoroughly investigated. Here, DC subpopulations from Lena in vivo infected pigs were analyzed for viral genome detection. This experiment demonstrates that cDC1, cDC2, and moDC are not infected in vivo by Lena. Analysis of DC cytokines production revealed that cDC1 are clearly activated in vivo by Lena. In vitro comparison of 3 Europeans strains revealed no infection of the cDC1 and cDC2 and no or little infection of moDC with Lena, whereas the two PRRSV-1.1 strains infect none of the 3 DC subtypes. In vitro investigation of T helper polarization and cytokines production demonstrate that Lena induces a higher Th1 polarization and IFNγ secretion than FL13 and LV. Altogether, this work suggests an activation of cDC1 by Lena associated with a Th1 immune response polarization.https://www.frontiersin.org/article/10.3389/fimmu.2018.02299/fullPRRSVLenalungdendritic cellscDC1Th1 response |
| spellingShingle | Elise Bordet Fany Blanc Mathieu Tiret Elisa Crisci Elisa Crisci Edwige Bouguyon Patricia Renson Patricia Renson Patricia Renson Pauline Maisonnasse Mickael Bourge Jean-Jacques Leplat Elisabetta Giuffra Luc Jouneau Isabelle Schwartz-Cornil Olivier Bourry Olivier Bourry Nicolas Bertho Porcine Reproductive and Respiratory Syndrome Virus Type 1.3 Lena Triggers Conventional Dendritic Cells 1 Activation and T Helper 1 Immune Response Without Infecting Dendritic Cells Frontiers in Immunology PRRSV Lena lung dendritic cells cDC1 Th1 response |
| title | Porcine Reproductive and Respiratory Syndrome Virus Type 1.3 Lena Triggers Conventional Dendritic Cells 1 Activation and T Helper 1 Immune Response Without Infecting Dendritic Cells |
| title_full | Porcine Reproductive and Respiratory Syndrome Virus Type 1.3 Lena Triggers Conventional Dendritic Cells 1 Activation and T Helper 1 Immune Response Without Infecting Dendritic Cells |
| title_fullStr | Porcine Reproductive and Respiratory Syndrome Virus Type 1.3 Lena Triggers Conventional Dendritic Cells 1 Activation and T Helper 1 Immune Response Without Infecting Dendritic Cells |
| title_full_unstemmed | Porcine Reproductive and Respiratory Syndrome Virus Type 1.3 Lena Triggers Conventional Dendritic Cells 1 Activation and T Helper 1 Immune Response Without Infecting Dendritic Cells |
| title_short | Porcine Reproductive and Respiratory Syndrome Virus Type 1.3 Lena Triggers Conventional Dendritic Cells 1 Activation and T Helper 1 Immune Response Without Infecting Dendritic Cells |
| title_sort | porcine reproductive and respiratory syndrome virus type 1 3 lena triggers conventional dendritic cells 1 activation and t helper 1 immune response without infecting dendritic cells |
| topic | PRRSV Lena lung dendritic cells cDC1 Th1 response |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2018.02299/full |
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