Epidemiologic aspects of an emerging focus of visceral leishmaniasis in Tbilisi, Georgia.
BACKGROUND: Over the last 15 years, visceral leishmaniasis (VL) has emerged as a public health concern in Tbilisi, the capital of Georgia. METHODOLOGY/PRINCIPAL FINDINGS: Seroepidemiological surveys were conducted to determine the prevalence and incidence of infection in children and dogs within the...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-12-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | http://europepmc.org/articles/PMC3236723?pdf=render |
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| author | Ekaterina Giorgobiani Nazibrola Chitadze Gvantsa Chanturya Marina Grdzelidze Ryan C Jochim Anna Machablishvili Tsiuri Tushishvili Yulia Zedginidze Marina K Manjgaladze Nino Iashvili Manana P Makharadze Tsiuri Zakaraya Konstantin Kikaleishvili Ivan Markhvashvili Goderdzi Badashvili Teymuraz Daraselia Michael P Fay Shaden Kamhawi David Sacks |
| author_facet | Ekaterina Giorgobiani Nazibrola Chitadze Gvantsa Chanturya Marina Grdzelidze Ryan C Jochim Anna Machablishvili Tsiuri Tushishvili Yulia Zedginidze Marina K Manjgaladze Nino Iashvili Manana P Makharadze Tsiuri Zakaraya Konstantin Kikaleishvili Ivan Markhvashvili Goderdzi Badashvili Teymuraz Daraselia Michael P Fay Shaden Kamhawi David Sacks |
| author_sort | Ekaterina Giorgobiani |
| collection | DOAJ |
| description | BACKGROUND: Over the last 15 years, visceral leishmaniasis (VL) has emerged as a public health concern in Tbilisi, the capital of Georgia. METHODOLOGY/PRINCIPAL FINDINGS: Seroepidemiological surveys were conducted to determine the prevalence and incidence of infection in children and dogs within the main focus of VL, and to identify risk factors associated with human infection. Of 4,250 children investigated, 7.3% were positive by direct agglutination test in a baseline survey; an apparent incidence rate of 6.0% was estimated by one year follow-up. None of the seropositive children progressed to VL during the survey. Increased seropositivity at one year was predicted by presence at baseline of clustered flying insects (OR = 1.49; P = 0.001), perceived satisfactory sanitation (OR = 1.65; P<0.001), stray dogs (OR = 1.33; P = 0.023), and by persistent fever during the 6 months prior to baseline survey (OR = 14.2; P<0.001). Overall, 18.2% (107/588) of domestic and 15.3% (110/718) of stray dogs were seropositive by the rk39 dipstick test. Clinical VL signs were found in 1.3% of domestic and 2.9% of stray, seropositive dogs. Parasites isolated from human and dog samples were identified by PCR and phylogenetic analysis of the Leishmania 70 kDa heat-shock protein (HSP70) gene as Leishmania infantum. CONCLUSIONS/SIGNIFICANCE: There is an active focus of L. infantum transmission in Tbilisi with a high prevalence of human and canine infections. |
| first_indexed | 2024-12-10T15:36:40Z |
| format | Article |
| id | doaj.art-6ee37f552b944809bc6d9aba6313f2a2 |
| institution | Directory Open Access Journal |
| issn | 1935-2735 |
| language | English |
| last_indexed | 2024-12-10T15:36:40Z |
| publishDate | 2011-12-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-6ee37f552b944809bc6d9aba6313f2a22022-12-22T01:43:12ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27352011-12-01512e141510.1371/journal.pntd.0001415Epidemiologic aspects of an emerging focus of visceral leishmaniasis in Tbilisi, Georgia.Ekaterina GiorgobianiNazibrola ChitadzeGvantsa ChanturyaMarina GrdzelidzeRyan C JochimAnna MachablishviliTsiuri TushishviliYulia ZedginidzeMarina K ManjgaladzeNino IashviliManana P MakharadzeTsiuri ZakarayaKonstantin KikaleishviliIvan MarkhvashviliGoderdzi BadashviliTeymuraz DaraseliaMichael P FayShaden KamhawiDavid SacksBACKGROUND: Over the last 15 years, visceral leishmaniasis (VL) has emerged as a public health concern in Tbilisi, the capital of Georgia. METHODOLOGY/PRINCIPAL FINDINGS: Seroepidemiological surveys were conducted to determine the prevalence and incidence of infection in children and dogs within the main focus of VL, and to identify risk factors associated with human infection. Of 4,250 children investigated, 7.3% were positive by direct agglutination test in a baseline survey; an apparent incidence rate of 6.0% was estimated by one year follow-up. None of the seropositive children progressed to VL during the survey. Increased seropositivity at one year was predicted by presence at baseline of clustered flying insects (OR = 1.49; P = 0.001), perceived satisfactory sanitation (OR = 1.65; P<0.001), stray dogs (OR = 1.33; P = 0.023), and by persistent fever during the 6 months prior to baseline survey (OR = 14.2; P<0.001). Overall, 18.2% (107/588) of domestic and 15.3% (110/718) of stray dogs were seropositive by the rk39 dipstick test. Clinical VL signs were found in 1.3% of domestic and 2.9% of stray, seropositive dogs. Parasites isolated from human and dog samples were identified by PCR and phylogenetic analysis of the Leishmania 70 kDa heat-shock protein (HSP70) gene as Leishmania infantum. CONCLUSIONS/SIGNIFICANCE: There is an active focus of L. infantum transmission in Tbilisi with a high prevalence of human and canine infections.http://europepmc.org/articles/PMC3236723?pdf=render |
| spellingShingle | Ekaterina Giorgobiani Nazibrola Chitadze Gvantsa Chanturya Marina Grdzelidze Ryan C Jochim Anna Machablishvili Tsiuri Tushishvili Yulia Zedginidze Marina K Manjgaladze Nino Iashvili Manana P Makharadze Tsiuri Zakaraya Konstantin Kikaleishvili Ivan Markhvashvili Goderdzi Badashvili Teymuraz Daraselia Michael P Fay Shaden Kamhawi David Sacks Epidemiologic aspects of an emerging focus of visceral leishmaniasis in Tbilisi, Georgia. PLoS Neglected Tropical Diseases |
| title | Epidemiologic aspects of an emerging focus of visceral leishmaniasis in Tbilisi, Georgia. |
| title_full | Epidemiologic aspects of an emerging focus of visceral leishmaniasis in Tbilisi, Georgia. |
| title_fullStr | Epidemiologic aspects of an emerging focus of visceral leishmaniasis in Tbilisi, Georgia. |
| title_full_unstemmed | Epidemiologic aspects of an emerging focus of visceral leishmaniasis in Tbilisi, Georgia. |
| title_short | Epidemiologic aspects of an emerging focus of visceral leishmaniasis in Tbilisi, Georgia. |
| title_sort | epidemiologic aspects of an emerging focus of visceral leishmaniasis in tbilisi georgia |
| url | http://europepmc.org/articles/PMC3236723?pdf=render |
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