Diversity of CRISPR-Cas type II-A systems in Streptococcus anginosus
Streptococcus anginosus is a commensal Streptococcal species that is often associated with invasive bacterial infections. However, little is known about its molecular genetic background. Many Streptococcal species, including S. anginosus, harbor clustered regularly interspaced short palindromic repe...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1188671/full |
| _version_ | 1797803797354905600 |
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| author | Richard Bauer Dorina Haider Aline Grempels Rebecca Roscher Stefanie Mauerer Barbara Spellerberg |
| author_facet | Richard Bauer Dorina Haider Aline Grempels Rebecca Roscher Stefanie Mauerer Barbara Spellerberg |
| author_sort | Richard Bauer |
| collection | DOAJ |
| description | Streptococcus anginosus is a commensal Streptococcal species that is often associated with invasive bacterial infections. However, little is known about its molecular genetic background. Many Streptococcal species, including S. anginosus, harbor clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems. A CRISPR-Cas type II-A system as well as a type II-C system have been reported for this species. To characterize the CRISPR-Cas type II systems of S. anginosus in more detail, we conducted a phylogenetic analysis of Cas9 sequences from CRISPR-Cas type II systems with a special focus on streptococci and S. anginosus. In addition, a phylogenetic analysis of S. anginosus strains based on housekeeping genes included in MLST analysis, was performed. All analyzed Cas9 sequences of S. anginosus clustered with the Cas9 sequences of CRISPR type II-A systems, including the Cas9 sequences of S. anginosus strains reported to harbor a type II-C system. The Cas9 genes of the CRISPR-Cas type II-C systems of other bacterial species separated into a different cluster. Moreover, analyzing the CRISPR loci found in S. anginosus, two distinct csn2 genes could be detected, a short form showing high similarity to the canonical form of the csn2 gene present in S. pyogenes. The second CRISPR type II locus of S. anginosus contained a longer variant of csn2 with close similarities to a csn2 gene that has previously been described in Streptococcus thermophilus. Since CRISPR-Cas type II-C systems do not contain a csn2 gene, the S. anginosus strains reported to have a CRISPR-Cas type II-C system appear to carry a variation of CRISPR-Cas type II-A harboring a long variant of csn2. |
| first_indexed | 2024-03-13T05:26:27Z |
| format | Article |
| id | doaj.art-6ee6dc3208aa4a589d60620138dba11b |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-13T05:26:27Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-6ee6dc3208aa4a589d60620138dba11b2023-06-15T05:38:57ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-06-011410.3389/fmicb.2023.11886711188671Diversity of CRISPR-Cas type II-A systems in Streptococcus anginosusRichard BauerDorina HaiderAline GrempelsRebecca RoscherStefanie MauererBarbara SpellerbergStreptococcus anginosus is a commensal Streptococcal species that is often associated with invasive bacterial infections. However, little is known about its molecular genetic background. Many Streptococcal species, including S. anginosus, harbor clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems. A CRISPR-Cas type II-A system as well as a type II-C system have been reported for this species. To characterize the CRISPR-Cas type II systems of S. anginosus in more detail, we conducted a phylogenetic analysis of Cas9 sequences from CRISPR-Cas type II systems with a special focus on streptococci and S. anginosus. In addition, a phylogenetic analysis of S. anginosus strains based on housekeeping genes included in MLST analysis, was performed. All analyzed Cas9 sequences of S. anginosus clustered with the Cas9 sequences of CRISPR type II-A systems, including the Cas9 sequences of S. anginosus strains reported to harbor a type II-C system. The Cas9 genes of the CRISPR-Cas type II-C systems of other bacterial species separated into a different cluster. Moreover, analyzing the CRISPR loci found in S. anginosus, two distinct csn2 genes could be detected, a short form showing high similarity to the canonical form of the csn2 gene present in S. pyogenes. The second CRISPR type II locus of S. anginosus contained a longer variant of csn2 with close similarities to a csn2 gene that has previously been described in Streptococcus thermophilus. Since CRISPR-Cas type II-C systems do not contain a csn2 gene, the S. anginosus strains reported to have a CRISPR-Cas type II-C system appear to carry a variation of CRISPR-Cas type II-A harboring a long variant of csn2.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1188671/fullStreptococcus anginosusCRISPR-Casclassificationphylogenetic analysisCSN2 gene |
| spellingShingle | Richard Bauer Dorina Haider Aline Grempels Rebecca Roscher Stefanie Mauerer Barbara Spellerberg Diversity of CRISPR-Cas type II-A systems in Streptococcus anginosus Frontiers in Microbiology Streptococcus anginosus CRISPR-Cas classification phylogenetic analysis CSN2 gene |
| title | Diversity of CRISPR-Cas type II-A systems in Streptococcus anginosus |
| title_full | Diversity of CRISPR-Cas type II-A systems in Streptococcus anginosus |
| title_fullStr | Diversity of CRISPR-Cas type II-A systems in Streptococcus anginosus |
| title_full_unstemmed | Diversity of CRISPR-Cas type II-A systems in Streptococcus anginosus |
| title_short | Diversity of CRISPR-Cas type II-A systems in Streptococcus anginosus |
| title_sort | diversity of crispr cas type ii a systems in streptococcus anginosus |
| topic | Streptococcus anginosus CRISPR-Cas classification phylogenetic analysis CSN2 gene |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1188671/full |
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