The HMGB Protein <i>Kl</i>Ixr1, a DNA Binding Regulator of <i>Kluyveromyces lactis</i> Gene Expression Involved in Oxidative Metabolism, Growth, and dNTP Synthesis
In the traditional fermentative model yeast <i>Saccharomyces cerevisiae</i>, <i>Sc</i>Ixr1 is an HMGB (High Mobility Group box B) protein that has been considered as an important regulator of gene transcription in response to external changes like oxygen, carbon source, or nu...
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MDPI AG
2021-09-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/9/1392 |
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| author | Agustín Rico-Díaz Aída Barreiro-Alonso Cora Rey-Souto Manuel Becerra Mónica Lamas-Maceiras M. Esperanza Cerdán Ángel Vizoso-Vázquez |
| author_facet | Agustín Rico-Díaz Aída Barreiro-Alonso Cora Rey-Souto Manuel Becerra Mónica Lamas-Maceiras M. Esperanza Cerdán Ángel Vizoso-Vázquez |
| author_sort | Agustín Rico-Díaz |
| collection | DOAJ |
| description | In the traditional fermentative model yeast <i>Saccharomyces cerevisiae</i>, <i>Sc</i>Ixr1 is an HMGB (High Mobility Group box B) protein that has been considered as an important regulator of gene transcription in response to external changes like oxygen, carbon source, or nutrient availability. <i>Kluyveromyces lactis</i> is also a useful eukaryotic model, more similar to many human cells due to its respiratory metabolism. We cloned and functionally characterized by different methodologies <i>Kl</i>IXR1, which encodes a protein with only 34.4% amino acid sequence similarity to <i>Sc</i>Ixr1. Our data indicate that both proteins share common functions, including their involvement in the response to hypoxia or oxidative stress induced by hydrogen peroxide or metal treatments, as well as in the control of key regulators for maintenance of the dNTP (deoxyribonucleotide triphosphate) pool and ribosome synthesis. <i>Kl</i>Ixr1 is able to bind specific regulatory DNA sequences in the promoter of its target genes, which are well conserved between <i>S. cerevisiae</i> and <i>K. lactis</i>. Oppositely, we found important differences between <i>Sc</i>Irx1 and <i>Kl</i>Ixr1 affecting cellular responses to cisplatin or cycloheximide in these yeasts, which could be dependent on specific and non-conserved domains present in these two proteins. |
| first_indexed | 2024-03-10T07:51:15Z |
| format | Article |
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| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T07:51:15Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-6ee780fb387d4c5c9a7b8fcffeb15c0d2023-11-22T12:12:02ZengMDPI AGBiomolecules2218-273X2021-09-01119139210.3390/biom11091392The HMGB Protein <i>Kl</i>Ixr1, a DNA Binding Regulator of <i>Kluyveromyces lactis</i> Gene Expression Involved in Oxidative Metabolism, Growth, and dNTP SynthesisAgustín Rico-Díaz0Aída Barreiro-Alonso1Cora Rey-Souto2Manuel Becerra3Mónica Lamas-Maceiras4M. Esperanza Cerdán5Ángel Vizoso-Vázquez6Grupo EXPRELA, Centro de Investigacións Científicas Avanzadas (CICA), Departamento de Bioloxía, Facultade de Ciencias, INIBIC—Universidade de A Coruña, Campus de A Zapateira, 15071 A Coruña, SpainGrupo EXPRELA, Centro de Investigacións Científicas Avanzadas (CICA), Departamento de Bioloxía, Facultade de Ciencias, INIBIC—Universidade de A Coruña, Campus de A Zapateira, 15071 A Coruña, SpainGrupo EXPRELA, Centro de Investigacións Científicas Avanzadas (CICA), Departamento de Bioloxía, Facultade de Ciencias, INIBIC—Universidade de A Coruña, Campus de A Zapateira, 15071 A Coruña, SpainGrupo EXPRELA, Centro de Investigacións Científicas Avanzadas (CICA), Departamento de Bioloxía, Facultade de Ciencias, INIBIC—Universidade de A Coruña, Campus de A Zapateira, 15071 A Coruña, SpainGrupo EXPRELA, Centro de Investigacións Científicas Avanzadas (CICA), Departamento de Bioloxía, Facultade de Ciencias, INIBIC—Universidade de A Coruña, Campus de A Zapateira, 15071 A Coruña, SpainGrupo EXPRELA, Centro de Investigacións Científicas Avanzadas (CICA), Departamento de Bioloxía, Facultade de Ciencias, INIBIC—Universidade de A Coruña, Campus de A Zapateira, 15071 A Coruña, SpainGrupo EXPRELA, Centro de Investigacións Científicas Avanzadas (CICA), Departamento de Bioloxía, Facultade de Ciencias, INIBIC—Universidade de A Coruña, Campus de A Zapateira, 15071 A Coruña, SpainIn the traditional fermentative model yeast <i>Saccharomyces cerevisiae</i>, <i>Sc</i>Ixr1 is an HMGB (High Mobility Group box B) protein that has been considered as an important regulator of gene transcription in response to external changes like oxygen, carbon source, or nutrient availability. <i>Kluyveromyces lactis</i> is also a useful eukaryotic model, more similar to many human cells due to its respiratory metabolism. We cloned and functionally characterized by different methodologies <i>Kl</i>IXR1, which encodes a protein with only 34.4% amino acid sequence similarity to <i>Sc</i>Ixr1. Our data indicate that both proteins share common functions, including their involvement in the response to hypoxia or oxidative stress induced by hydrogen peroxide or metal treatments, as well as in the control of key regulators for maintenance of the dNTP (deoxyribonucleotide triphosphate) pool and ribosome synthesis. <i>Kl</i>Ixr1 is able to bind specific regulatory DNA sequences in the promoter of its target genes, which are well conserved between <i>S. cerevisiae</i> and <i>K. lactis</i>. Oppositely, we found important differences between <i>Sc</i>Irx1 and <i>Kl</i>Ixr1 affecting cellular responses to cisplatin or cycloheximide in these yeasts, which could be dependent on specific and non-conserved domains present in these two proteins.https://www.mdpi.com/2218-273X/11/9/1392<i>Kluyveromyces lactis</i>IXR1cisplatin sensitivityhypoxiadNTP pool |
| spellingShingle | Agustín Rico-Díaz Aída Barreiro-Alonso Cora Rey-Souto Manuel Becerra Mónica Lamas-Maceiras M. Esperanza Cerdán Ángel Vizoso-Vázquez The HMGB Protein <i>Kl</i>Ixr1, a DNA Binding Regulator of <i>Kluyveromyces lactis</i> Gene Expression Involved in Oxidative Metabolism, Growth, and dNTP Synthesis Biomolecules <i>Kluyveromyces lactis</i> IXR1 cisplatin sensitivity hypoxia dNTP pool |
| title | The HMGB Protein <i>Kl</i>Ixr1, a DNA Binding Regulator of <i>Kluyveromyces lactis</i> Gene Expression Involved in Oxidative Metabolism, Growth, and dNTP Synthesis |
| title_full | The HMGB Protein <i>Kl</i>Ixr1, a DNA Binding Regulator of <i>Kluyveromyces lactis</i> Gene Expression Involved in Oxidative Metabolism, Growth, and dNTP Synthesis |
| title_fullStr | The HMGB Protein <i>Kl</i>Ixr1, a DNA Binding Regulator of <i>Kluyveromyces lactis</i> Gene Expression Involved in Oxidative Metabolism, Growth, and dNTP Synthesis |
| title_full_unstemmed | The HMGB Protein <i>Kl</i>Ixr1, a DNA Binding Regulator of <i>Kluyveromyces lactis</i> Gene Expression Involved in Oxidative Metabolism, Growth, and dNTP Synthesis |
| title_short | The HMGB Protein <i>Kl</i>Ixr1, a DNA Binding Regulator of <i>Kluyveromyces lactis</i> Gene Expression Involved in Oxidative Metabolism, Growth, and dNTP Synthesis |
| title_sort | hmgb protein i kl i ixr1 a dna binding regulator of i kluyveromyces lactis i gene expression involved in oxidative metabolism growth and dntp synthesis |
| topic | <i>Kluyveromyces lactis</i> IXR1 cisplatin sensitivity hypoxia dNTP pool |
| url | https://www.mdpi.com/2218-273X/11/9/1392 |
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