EFFECT OF EXTRACHROMOSOMAL ELEMENTS OF HEREDITY ON TOXIC PROPERTIES OF YERSINIA PESTIS
Aim. Elucidation of the role of extrachromosomal elements of heredity in manifestations of toxic properties of Yersinia pestis. Materials and methods. The study was carried out in vaccine strain Y. pestis EV76 (pMTl, pCDl, pPCPl) and non-plasmid variants of vaccine EV76 (pMTl\ pCDl', pPCPl'...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Central Research Institute for Epidemiology
2017-04-01
|
| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
| Subjects: | |
| Online Access: | https://microbiol.elpub.ru/jour/article/view/138 |
| _version_ | 1798036232833335296 |
|---|---|
| author | G. V. Demidova E. P. Sokolova V. P. Zyuzina V. A. Rykova I. V. Morozova O. N. Podladchikova V. I. Tynyanova |
| author_facet | G. V. Demidova E. P. Sokolova V. P. Zyuzina V. A. Rykova I. V. Morozova O. N. Podladchikova V. I. Tynyanova |
| author_sort | G. V. Demidova |
| collection | DOAJ |
| description | Aim. Elucidation of the role of extrachromosomal elements of heredity in manifestations of toxic properties of Yersinia pestis. Materials and methods. The study was carried out in vaccine strain Y. pestis EV76 (pMTl, pCDl, pPCPl) and non-plasmid variants of vaccine EV76 (pMTl\ pCDl', pPCPl') and virulent 231 (рМТГ, pCDl’, pPCPl') strains of Y. pestis. Presence of functionally active form of lipopolysaccharide (LPS) in the incubation medium of the bacteria was evaluated via toxicity of supernatant of Y. pestis for intact animals (infection-toxic shock) and mice sensitized by D-GalN. Results. 37°C cultures of Y. pestis EV76 containing a full amount of plasmids were established to release LPS into the environment. Non-plasmid variants of both vaccine and virulent strains of Y. pestis pMTl', pCD Г, рРСР 1 do not have this ability. Separation of LPS from cell wall was detected in live bacteria of plague infectious agent. This process is assumed to be coupled with translocation of proteins coded by pMTl, pCDl, pPCPl plasmids from the cell into the environment. Conclusion. Functional interconnection between extrachromosomal elements of heredity and toxic activity of Y. pestis LPS is established for the first time. |
| first_indexed | 2024-04-11T21:09:46Z |
| format | Article |
| id | doaj.art-6ee7be5404a746638b30190d6f4c62be |
| institution | Directory Open Access Journal |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| last_indexed | 2024-04-11T21:09:46Z |
| publishDate | 2017-04-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj.art-6ee7be5404a746638b30190d6f4c62be2022-12-22T04:03:04ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132017-04-0102283310.36233/0372-9311-2017-2-28-33138EFFECT OF EXTRACHROMOSOMAL ELEMENTS OF HEREDITY ON TOXIC PROPERTIES OF YERSINIA PESTISG. V. Demidova0E. P. Sokolova1V. P. Zyuzina2V. A. Rykova3I. V. Morozova4O. N. Podladchikova5V. I. Tynyanova6Rostov-on-Don Institute for Plague ControlRostov-on-Don Institute for Plague ControlRostov-on-Don Institute for Plague ControlRostov-on-Don Institute for Plague ControlRostov-on-Don Institute for Plague ControlRostov-on-Don Institute for Plague ControlRostov-on-Don Institute for Plague ControlAim. Elucidation of the role of extrachromosomal elements of heredity in manifestations of toxic properties of Yersinia pestis. Materials and methods. The study was carried out in vaccine strain Y. pestis EV76 (pMTl, pCDl, pPCPl) and non-plasmid variants of vaccine EV76 (pMTl\ pCDl', pPCPl') and virulent 231 (рМТГ, pCDl’, pPCPl') strains of Y. pestis. Presence of functionally active form of lipopolysaccharide (LPS) in the incubation medium of the bacteria was evaluated via toxicity of supernatant of Y. pestis for intact animals (infection-toxic shock) and mice sensitized by D-GalN. Results. 37°C cultures of Y. pestis EV76 containing a full amount of plasmids were established to release LPS into the environment. Non-plasmid variants of both vaccine and virulent strains of Y. pestis pMTl', pCD Г, рРСР 1 do not have this ability. Separation of LPS from cell wall was detected in live bacteria of plague infectious agent. This process is assumed to be coupled with translocation of proteins coded by pMTl, pCDl, pPCPl plasmids from the cell into the environment. Conclusion. Functional interconnection between extrachromosomal elements of heredity and toxic activity of Y. pestis LPS is established for the first time.https://microbiol.elpub.ru/jour/article/view/138yersinia pestisyersinia pestisplasmidslipopolysaccharidetoxicity |
| spellingShingle | G. V. Demidova E. P. Sokolova V. P. Zyuzina V. A. Rykova I. V. Morozova O. N. Podladchikova V. I. Tynyanova EFFECT OF EXTRACHROMOSOMAL ELEMENTS OF HEREDITY ON TOXIC PROPERTIES OF YERSINIA PESTIS Журнал микробиологии, эпидемиологии и иммунобиологии yersinia pestis yersinia pestis plasmids lipopolysaccharide toxicity |
| title | EFFECT OF EXTRACHROMOSOMAL ELEMENTS OF HEREDITY ON TOXIC PROPERTIES OF YERSINIA PESTIS |
| title_full | EFFECT OF EXTRACHROMOSOMAL ELEMENTS OF HEREDITY ON TOXIC PROPERTIES OF YERSINIA PESTIS |
| title_fullStr | EFFECT OF EXTRACHROMOSOMAL ELEMENTS OF HEREDITY ON TOXIC PROPERTIES OF YERSINIA PESTIS |
| title_full_unstemmed | EFFECT OF EXTRACHROMOSOMAL ELEMENTS OF HEREDITY ON TOXIC PROPERTIES OF YERSINIA PESTIS |
| title_short | EFFECT OF EXTRACHROMOSOMAL ELEMENTS OF HEREDITY ON TOXIC PROPERTIES OF YERSINIA PESTIS |
| title_sort | effect of extrachromosomal elements of heredity on toxic properties of yersinia pestis |
| topic | yersinia pestis yersinia pestis plasmids lipopolysaccharide toxicity |
| url | https://microbiol.elpub.ru/jour/article/view/138 |
| work_keys_str_mv | AT gvdemidova effectofextrachromosomalelementsofheredityontoxicpropertiesofyersiniapestis AT epsokolova effectofextrachromosomalelementsofheredityontoxicpropertiesofyersiniapestis AT vpzyuzina effectofextrachromosomalelementsofheredityontoxicpropertiesofyersiniapestis AT varykova effectofextrachromosomalelementsofheredityontoxicpropertiesofyersiniapestis AT ivmorozova effectofextrachromosomalelementsofheredityontoxicpropertiesofyersiniapestis AT onpodladchikova effectofextrachromosomalelementsofheredityontoxicpropertiesofyersiniapestis AT vitynyanova effectofextrachromosomalelementsofheredityontoxicpropertiesofyersiniapestis |