The Potential Role of Sulbactam and Cephalosporins Plus Daptomycin Against Daptomycin-Nonsusceptible VISA and H-VISA Isolates: An In Vitro Study
This study assesses the synergistic effect of the combination of cephalosporins and sulbactam with daptomycin against daptomycin-nonsusceptible, vancomycin-intermediate resistant <i>Staphylococcus aureus</i> (VISA) or heterogeneous vancomycin-intermediate <i>S. aureus</i> (h-...
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| Format: | Article |
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MDPI AG
2019-10-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/8/4/184 |
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| author | Chih-Cheng Lai Chi-Chung Chen Ying-Chen Lu Tsuey-Pin Lin Hung-Jui Chen Bo-An Su Chien-Ming Chao Yin-Ching Chuang Hung-Jen Tang |
| author_facet | Chih-Cheng Lai Chi-Chung Chen Ying-Chen Lu Tsuey-Pin Lin Hung-Jui Chen Bo-An Su Chien-Ming Chao Yin-Ching Chuang Hung-Jen Tang |
| author_sort | Chih-Cheng Lai |
| collection | DOAJ |
| description | This study assesses the synergistic effect of the combination of cephalosporins and sulbactam with daptomycin against daptomycin-nonsusceptible, vancomycin-intermediate resistant <i>Staphylococcus aureus</i> (VISA) or heterogeneous vancomycin-intermediate <i>S. aureus</i> (h-VISA) isolates. The in vitro activity of daptomycin against daptomycin-nonsusceptible VISA/h-VISA isolates after adding cephalosporins with or without sulbactam was evaluated. The MIC of daptomycin against the VISA/h-VISA isolates was reduced after adding cephalosporins to daptomycin. Except for one VISA and two h-VISA isolates, the other VISA/h-VISA isolates became daptomycin-susceptible (MICs ≤ 1 mg/L). After adding sulbactam to each daptomycin/cephalosporin combination, the MIC of daptomycin against the VISA/h-VISA isolates decreased for 5 (33.3%), 6 (40.0%), 6 (40.0%), and 6 (40.0%) isolates with the cefazolin, cefmetazole, cefotaxime, and cefepime combinations, respectively. Synergism using the checkerboard method was noted in 100% of cefazolin and cefotaxime combinations and 87% and 80% of cefmetazole and cefepime combinations for all the VISA and h-VISA isolates. With the addition of sulbactam, synergism was noted in 100% of cefazolin, cefmetazole, and cefotaxime combinations and 93% of the cefepime combinations for all the VISA and h-VISA isolates. Almost all the FICs for the three-drug combinations were lower than those for the two-drug combinations. Using time-killing methods, a synergistic effect against five h-VISA isolates was observed. A synergistic effect of daptomycin, sulbactam, and each cephalosporin was observed for all VISA isolates. In conclusion, the activity of daptomycin against daptomycin-nonsusceptible VISA/h-VISA isolates can be enhanced by adding cephalosporins, and partially further promoted by sulbactam. |
| first_indexed | 2024-12-10T06:21:00Z |
| format | Article |
| id | doaj.art-6ee9bff5ea8b4242847dd3d70af545b7 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-12-10T06:21:00Z |
| publishDate | 2019-10-01 |
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| series | Antibiotics |
| spelling | doaj.art-6ee9bff5ea8b4242847dd3d70af545b72022-12-22T01:59:21ZengMDPI AGAntibiotics2079-63822019-10-018418410.3390/antibiotics8040184antibiotics8040184The Potential Role of Sulbactam and Cephalosporins Plus Daptomycin Against Daptomycin-Nonsusceptible VISA and H-VISA Isolates: An In Vitro StudyChih-Cheng Lai0Chi-Chung Chen1Ying-Chen Lu2Tsuey-Pin Lin3Hung-Jui Chen4Bo-An Su5Chien-Ming Chao6Yin-Ching Chuang7Hung-Jen Tang8Department of Internal Medicine, Kaohsiung Veterans General Hospital, Tainan Branch, Tainan 71051, TaiwanDepartment of Medical Research; Chi Mei Medical Center; Tainan 71004, TaiwanDepartment of Food Science, National Chiayi University, Chiayi 60004, TaiwanDepartment of Health and Nutrition, Chia-Nan University of Pharmacy and Science, Tainan 71710, TaiwanDepartment of Medicine, Chi Mei Medical Center, Tainan 71004, TaiwanDepartment of Medicine, Chi Mei Medical Center, Tainan 71004, TaiwanDepartment of Intensive Care Medicine, Chi Mei Medical Center, Liouying, Tainan 73657, TaiwanDepartment of Medical Research; Chi Mei Medical Center; Tainan 71004, TaiwanDepartment of Health and Nutrition, Chia-Nan University of Pharmacy and Science, Tainan 71710, TaiwanThis study assesses the synergistic effect of the combination of cephalosporins and sulbactam with daptomycin against daptomycin-nonsusceptible, vancomycin-intermediate resistant <i>Staphylococcus aureus</i> (VISA) or heterogeneous vancomycin-intermediate <i>S. aureus</i> (h-VISA) isolates. The in vitro activity of daptomycin against daptomycin-nonsusceptible VISA/h-VISA isolates after adding cephalosporins with or without sulbactam was evaluated. The MIC of daptomycin against the VISA/h-VISA isolates was reduced after adding cephalosporins to daptomycin. Except for one VISA and two h-VISA isolates, the other VISA/h-VISA isolates became daptomycin-susceptible (MICs ≤ 1 mg/L). After adding sulbactam to each daptomycin/cephalosporin combination, the MIC of daptomycin against the VISA/h-VISA isolates decreased for 5 (33.3%), 6 (40.0%), 6 (40.0%), and 6 (40.0%) isolates with the cefazolin, cefmetazole, cefotaxime, and cefepime combinations, respectively. Synergism using the checkerboard method was noted in 100% of cefazolin and cefotaxime combinations and 87% and 80% of cefmetazole and cefepime combinations for all the VISA and h-VISA isolates. With the addition of sulbactam, synergism was noted in 100% of cefazolin, cefmetazole, and cefotaxime combinations and 93% of the cefepime combinations for all the VISA and h-VISA isolates. Almost all the FICs for the three-drug combinations were lower than those for the two-drug combinations. Using time-killing methods, a synergistic effect against five h-VISA isolates was observed. A synergistic effect of daptomycin, sulbactam, and each cephalosporin was observed for all VISA isolates. In conclusion, the activity of daptomycin against daptomycin-nonsusceptible VISA/h-VISA isolates can be enhanced by adding cephalosporins, and partially further promoted by sulbactam.https://www.mdpi.com/2079-6382/8/4/184vancomycin-intermediate resistant <i>s. aureus</i>heterogeneous vancomycin-intermediate <i>s. aureus</i>daptomycincephalosporinsulbactamsynergism test |
| spellingShingle | Chih-Cheng Lai Chi-Chung Chen Ying-Chen Lu Tsuey-Pin Lin Hung-Jui Chen Bo-An Su Chien-Ming Chao Yin-Ching Chuang Hung-Jen Tang The Potential Role of Sulbactam and Cephalosporins Plus Daptomycin Against Daptomycin-Nonsusceptible VISA and H-VISA Isolates: An In Vitro Study Antibiotics vancomycin-intermediate resistant <i>s. aureus</i> heterogeneous vancomycin-intermediate <i>s. aureus</i> daptomycin cephalosporin sulbactam synergism test |
| title | The Potential Role of Sulbactam and Cephalosporins Plus Daptomycin Against Daptomycin-Nonsusceptible VISA and H-VISA Isolates: An In Vitro Study |
| title_full | The Potential Role of Sulbactam and Cephalosporins Plus Daptomycin Against Daptomycin-Nonsusceptible VISA and H-VISA Isolates: An In Vitro Study |
| title_fullStr | The Potential Role of Sulbactam and Cephalosporins Plus Daptomycin Against Daptomycin-Nonsusceptible VISA and H-VISA Isolates: An In Vitro Study |
| title_full_unstemmed | The Potential Role of Sulbactam and Cephalosporins Plus Daptomycin Against Daptomycin-Nonsusceptible VISA and H-VISA Isolates: An In Vitro Study |
| title_short | The Potential Role of Sulbactam and Cephalosporins Plus Daptomycin Against Daptomycin-Nonsusceptible VISA and H-VISA Isolates: An In Vitro Study |
| title_sort | potential role of sulbactam and cephalosporins plus daptomycin against daptomycin nonsusceptible visa and h visa isolates an in vitro study |
| topic | vancomycin-intermediate resistant <i>s. aureus</i> heterogeneous vancomycin-intermediate <i>s. aureus</i> daptomycin cephalosporin sulbactam synergism test |
| url | https://www.mdpi.com/2079-6382/8/4/184 |
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