Knockdown of ZNF479 inhibits proliferation and glycolysis of gastric cancer cells through regulating β‐catenin/c‐Myc signaling pathway
Abstract Gastric cancer is the fifth most common malignancy and the third most deadly tumor in the world. Zinc finger protein 479 (ZNF479) has been demonstrated to play crucial roles in hepatocellular carcinoma. However, the function of ZNF479 in gastric cancer remains to be clarified. The current s...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-09-01
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| Series: | Kaohsiung Journal of Medical Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/kjm2.12399 |
| _version_ | 1831585976069128192 |
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| author | Xiao‐Sheng Jin Ting‐Ting Ji Zheng‐Chao Shi Qing‐Qing Zhang Fang‐Peng Ye Wei‐Lai Yu Rong‐Zhou Li |
| author_facet | Xiao‐Sheng Jin Ting‐Ting Ji Zheng‐Chao Shi Qing‐Qing Zhang Fang‐Peng Ye Wei‐Lai Yu Rong‐Zhou Li |
| author_sort | Xiao‐Sheng Jin |
| collection | DOAJ |
| description | Abstract Gastric cancer is the fifth most common malignancy and the third most deadly tumor in the world. Zinc finger protein 479 (ZNF479) has been demonstrated to play crucial roles in hepatocellular carcinoma. However, the function of ZNF479 in gastric cancer remains to be clarified. The current study aimed to investigate the role of ZNF479 in gastric cancer progression and elucidate the potential molecular mechanism. In this study, Cell Count Kit‐8 and colony formation assays demonstrated that knockdown of ZNF479 inhibited cell proliferation in AGS and SGC‐7901 cells. Of note, knockdown of ZNF479 hinders tumor growth of xenograft tumor mice. What is more, knockdown of ZNF479 inhibited glucose uptake, lactate production, adenosine triphosphate level, and extracellular acidification ratio; increased oxygen consumption ratio in gastric cancer cells; and decreased the expression of glycolytic proteins both in vitro and in vivo. Furthermore, analysis mechanism suggests that ZNF479 participated in the regulation of gastric cancer progression through affecting the β‐catenin/c‐Myc signaling pathway. Collectively, ZNF479 plays a role as an oncogene through modulating β‐catenin/c‐Myc signaling pathway in the development of gastric cancer, which provides a new research target for future studies. |
| first_indexed | 2024-12-17T21:28:20Z |
| format | Article |
| id | doaj.art-6ef02df05cde4653af5e0b0afadd612c |
| institution | Directory Open Access Journal |
| issn | 1607-551X 2410-8650 |
| language | English |
| last_indexed | 2024-12-17T21:28:20Z |
| publishDate | 2021-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Kaohsiung Journal of Medical Sciences |
| spelling | doaj.art-6ef02df05cde4653af5e0b0afadd612c2022-12-21T21:31:58ZengWileyKaohsiung Journal of Medical Sciences1607-551X2410-86502021-09-0137975976710.1002/kjm2.12399Knockdown of ZNF479 inhibits proliferation and glycolysis of gastric cancer cells through regulating β‐catenin/c‐Myc signaling pathwayXiao‐Sheng Jin0Ting‐Ting Ji1Zheng‐Chao Shi2Qing‐Qing Zhang3Fang‐Peng Ye4Wei‐Lai Yu5Rong‐Zhou Li6Department of Gastroenterology Ruian people's hospital Rui'an City ChinaDepartment of Gastroenterology Ruian people's hospital Rui'an City ChinaDepartment of Gastroenterology Ruian people's hospital Rui'an City ChinaDepartment of Gastroenterology Ruian people's hospital Rui'an City ChinaDepartment of Gastroenterology Ruian people's hospital Rui'an City ChinaDepartment of Gastroenterology Ruian people's hospital Rui'an City ChinaDepartment of Gastroenterology Ruian people's hospital Rui'an City ChinaAbstract Gastric cancer is the fifth most common malignancy and the third most deadly tumor in the world. Zinc finger protein 479 (ZNF479) has been demonstrated to play crucial roles in hepatocellular carcinoma. However, the function of ZNF479 in gastric cancer remains to be clarified. The current study aimed to investigate the role of ZNF479 in gastric cancer progression and elucidate the potential molecular mechanism. In this study, Cell Count Kit‐8 and colony formation assays demonstrated that knockdown of ZNF479 inhibited cell proliferation in AGS and SGC‐7901 cells. Of note, knockdown of ZNF479 hinders tumor growth of xenograft tumor mice. What is more, knockdown of ZNF479 inhibited glucose uptake, lactate production, adenosine triphosphate level, and extracellular acidification ratio; increased oxygen consumption ratio in gastric cancer cells; and decreased the expression of glycolytic proteins both in vitro and in vivo. Furthermore, analysis mechanism suggests that ZNF479 participated in the regulation of gastric cancer progression through affecting the β‐catenin/c‐Myc signaling pathway. Collectively, ZNF479 plays a role as an oncogene through modulating β‐catenin/c‐Myc signaling pathway in the development of gastric cancer, which provides a new research target for future studies.https://doi.org/10.1002/kjm2.12399c‐Mycgastric cancerglycolysisproliferationZNF479 |
| spellingShingle | Xiao‐Sheng Jin Ting‐Ting Ji Zheng‐Chao Shi Qing‐Qing Zhang Fang‐Peng Ye Wei‐Lai Yu Rong‐Zhou Li Knockdown of ZNF479 inhibits proliferation and glycolysis of gastric cancer cells through regulating β‐catenin/c‐Myc signaling pathway Kaohsiung Journal of Medical Sciences c‐Myc gastric cancer glycolysis proliferation ZNF479 |
| title | Knockdown of ZNF479 inhibits proliferation and glycolysis of gastric cancer cells through regulating β‐catenin/c‐Myc signaling pathway |
| title_full | Knockdown of ZNF479 inhibits proliferation and glycolysis of gastric cancer cells through regulating β‐catenin/c‐Myc signaling pathway |
| title_fullStr | Knockdown of ZNF479 inhibits proliferation and glycolysis of gastric cancer cells through regulating β‐catenin/c‐Myc signaling pathway |
| title_full_unstemmed | Knockdown of ZNF479 inhibits proliferation and glycolysis of gastric cancer cells through regulating β‐catenin/c‐Myc signaling pathway |
| title_short | Knockdown of ZNF479 inhibits proliferation and glycolysis of gastric cancer cells through regulating β‐catenin/c‐Myc signaling pathway |
| title_sort | knockdown of znf479 inhibits proliferation and glycolysis of gastric cancer cells through regulating β catenin c myc signaling pathway |
| topic | c‐Myc gastric cancer glycolysis proliferation ZNF479 |
| url | https://doi.org/10.1002/kjm2.12399 |
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