Differential expression of the KLK2 and KLK3 genes in peripheral blood and tissues of patients with prostate cancer
We used the multiplex semi-quantitative reverse-transcriptase PCR (RT-PCR) to investigate kallikrein 2 and 3 (KLK2 and KLK3) mRNA levels in prostate tissue from 42 prostate cancer patients, 33 of whom were also assessed for peripheral blood KLK2 expression by qualitative semi-nested RT-PCR. We found...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2006-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200001 |
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| author | Juliana Meola Luiz R. Goulart Jaqueline D.D. Oliveira Adriana F. Neves Waldesse P. Oliveira Jr. Ana C.M. Saraiva Andréia C. Capaneli Alexandra M. Cardoso Lindolfo D. Prado Sebastião A. Borba Heyder D. Silva |
| author_facet | Juliana Meola Luiz R. Goulart Jaqueline D.D. Oliveira Adriana F. Neves Waldesse P. Oliveira Jr. Ana C.M. Saraiva Andréia C. Capaneli Alexandra M. Cardoso Lindolfo D. Prado Sebastião A. Borba Heyder D. Silva |
| author_sort | Juliana Meola |
| collection | DOAJ |
| description | We used the multiplex semi-quantitative reverse-transcriptase PCR (RT-PCR) to investigate kallikrein 2 and 3 (KLK2 and KLK3) mRNA levels in prostate tissue from 42 prostate cancer patients, 33 of whom were also assessed for peripheral blood KLK2 expression by qualitative semi-nested RT-PCR. We found that KLK2 was an important tissue biomarker for distinguishing between prostate cancer patients and those with benign prostatic hyperplasia, particularly when KLK2 expression was > 60% of that of the beta2-microglobulin constitutive gene. Patients with an average relative expression value > 0.6 (cutoff value) had an eleven-fold higher chance of having prostate cancer. When one or two tissues samples were evaluated for KLK2 expression using the cutoff value the estimated chance of having prostate cancer was increased by seven times for one positive sample and 45 times for two positive samples. There was no significant correlation between KLK3 gene expression and prostate cancer diagnosis. Logistic regression for blood and tissue KLK2 expression successfully detected 92% of the prostate cancer cases. The detection of KLK2 in blood showed a sensitivity of 59% and a specificity of 82%. This study indicates that the KLK2 gene may be a useful molecular marker for the diagnosis of prostate cancer and that analysis of KLK2 expression in blood and tissues could provide a novel approach for the clinical investigation of this type of cancer. |
| first_indexed | 2024-12-13T23:09:21Z |
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| id | doaj.art-6ef375aac7064334ac4045efdeb6be44 |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-13T23:09:21Z |
| publishDate | 2006-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-6ef375aac7064334ac4045efdeb6be442022-12-21T23:28:09ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852006-01-0129219319910.1590/S1415-47572006000200001Differential expression of the KLK2 and KLK3 genes in peripheral blood and tissues of patients with prostate cancerJuliana MeolaLuiz R. GoulartJaqueline D.D. OliveiraAdriana F. NevesWaldesse P. Oliveira Jr.Ana C.M. SaraivaAndréia C. CapaneliAlexandra M. CardosoLindolfo D. PradoSebastião A. BorbaHeyder D. SilvaWe used the multiplex semi-quantitative reverse-transcriptase PCR (RT-PCR) to investigate kallikrein 2 and 3 (KLK2 and KLK3) mRNA levels in prostate tissue from 42 prostate cancer patients, 33 of whom were also assessed for peripheral blood KLK2 expression by qualitative semi-nested RT-PCR. We found that KLK2 was an important tissue biomarker for distinguishing between prostate cancer patients and those with benign prostatic hyperplasia, particularly when KLK2 expression was > 60% of that of the beta2-microglobulin constitutive gene. Patients with an average relative expression value > 0.6 (cutoff value) had an eleven-fold higher chance of having prostate cancer. When one or two tissues samples were evaluated for KLK2 expression using the cutoff value the estimated chance of having prostate cancer was increased by seven times for one positive sample and 45 times for two positive samples. There was no significant correlation between KLK3 gene expression and prostate cancer diagnosis. Logistic regression for blood and tissue KLK2 expression successfully detected 92% of the prostate cancer cases. The detection of KLK2 in blood showed a sensitivity of 59% and a specificity of 82%. This study indicates that the KLK2 gene may be a useful molecular marker for the diagnosis of prostate cancer and that analysis of KLK2 expression in blood and tissues could provide a novel approach for the clinical investigation of this type of cancer.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200001kallikrein IImolecular markersprostate cancerPSAsemi-quantitative RT-PCR |
| spellingShingle | Juliana Meola Luiz R. Goulart Jaqueline D.D. Oliveira Adriana F. Neves Waldesse P. Oliveira Jr. Ana C.M. Saraiva Andréia C. Capaneli Alexandra M. Cardoso Lindolfo D. Prado Sebastião A. Borba Heyder D. Silva Differential expression of the KLK2 and KLK3 genes in peripheral blood and tissues of patients with prostate cancer Genetics and Molecular Biology kallikrein II molecular markers prostate cancer PSA semi-quantitative RT-PCR |
| title | Differential expression of the KLK2 and KLK3 genes in peripheral blood and tissues of patients with prostate cancer |
| title_full | Differential expression of the KLK2 and KLK3 genes in peripheral blood and tissues of patients with prostate cancer |
| title_fullStr | Differential expression of the KLK2 and KLK3 genes in peripheral blood and tissues of patients with prostate cancer |
| title_full_unstemmed | Differential expression of the KLK2 and KLK3 genes in peripheral blood and tissues of patients with prostate cancer |
| title_short | Differential expression of the KLK2 and KLK3 genes in peripheral blood and tissues of patients with prostate cancer |
| title_sort | differential expression of the klk2 and klk3 genes in peripheral blood and tissues of patients with prostate cancer |
| topic | kallikrein II molecular markers prostate cancer PSA semi-quantitative RT-PCR |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200001 |
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