Chitosan (CS)/Hydroxyapatite (HA)/Tricalcium Phosphate (β-TCP)-Based Composites as a Potential Material for Pulp Tissue Regeneration
This research focused on developing new materials for endodontic treatments to restore tissues affected by infectious or inflammatory processes. Three materials were studied, namely tricalcium phosphate β-hydroxyapatite (β-TCP), commercial and natural hydroxyapatite (HA), and chitosan (CS), in diffe...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-07-01
|
| Series: | Polymers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4360/15/15/3213 |
| _version_ | 1797586096449650688 |
|---|---|
| author | Ingrid Zamora Gilbert Alfonso Morales Jorge Iván Castro Lina Marcela Ruiz Rojas Carlos Humberto Valencia-Llano Jose Herminsul Mina Hernandez Mayra Eliana Valencia Zapata Carlos David Grande-Tovar |
| author_facet | Ingrid Zamora Gilbert Alfonso Morales Jorge Iván Castro Lina Marcela Ruiz Rojas Carlos Humberto Valencia-Llano Jose Herminsul Mina Hernandez Mayra Eliana Valencia Zapata Carlos David Grande-Tovar |
| author_sort | Ingrid Zamora |
| collection | DOAJ |
| description | This research focused on developing new materials for endodontic treatments to restore tissues affected by infectious or inflammatory processes. Three materials were studied, namely tricalcium phosphate β-hydroxyapatite (β-TCP), commercial and natural hydroxyapatite (HA), and chitosan (CS), in different proportions. The chemical characterization using infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis confirmed the composition of the composite. Scanning electron microscopy (SEM) demonstrated that the design and origin of the HA, whether natural or commercial, did not affect the morphology of the composites. In vitro studies using <i>Artemia salina</i> (<i>A. salina</i>) indicated that all three experimental materials were biocompatible after 24 h, with no significant differences in mortality rate observed among the groups. The subdermal implantation of the materials in block form exhibited biocompatibility and biodegradability after 30 and 60 days, with the larger particles undergoing fragmentation and connective tissue formation consisting of collagen type III fibers, blood vessels, and inflammatory cells. The implanted material continued to undergo resorption during this process. The results obtained in this research contribute to developing endodontic technologies for tissue recovery and regeneration. |
| first_indexed | 2024-03-11T00:18:27Z |
| format | Article |
| id | doaj.art-6ef52a5a15c7468692d6198892e35fff |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-11T00:18:27Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-6ef52a5a15c7468692d6198892e35fff2023-11-18T23:28:08ZengMDPI AGPolymers2073-43602023-07-011515321310.3390/polym15153213Chitosan (CS)/Hydroxyapatite (HA)/Tricalcium Phosphate (β-TCP)-Based Composites as a Potential Material for Pulp Tissue RegenerationIngrid Zamora0Gilbert Alfonso Morales1Jorge Iván Castro2Lina Marcela Ruiz Rojas3Carlos Humberto Valencia-Llano4Jose Herminsul Mina Hernandez5Mayra Eliana Valencia Zapata6Carlos David Grande-Tovar7Grupo Biomateriales Dentales, Escuela de Odontología, Universidad del Valle, Calle 4B # 36-00, Cali 76001, ColombiaGrupo Biomateriales Dentales, Escuela de Odontología, Universidad del Valle, Calle 4B # 36-00, Cali 76001, ColombiaLaboratorio SIMERQO, Departamento de Química, Universidad del Valle, Calle 13 No. 100-00, Cali 76001, ColombiaGrupo de Materiales Compuestos, Escuela de Ingeniería de Materiales, Facultad de Ingeniería, Universidad del Valle, Calle 13 No. 100-00, Cali 760032, ColombiaGrupo Biomateriales Dentales, Escuela de Odontología, Universidad del Valle, Calle 4B # 36-00, Cali 76001, ColombiaGrupo de Materiales Compuestos, Escuela de Ingeniería de Materiales, Facultad de Ingeniería, Universidad del Valle, Calle 13 No. 100-00, Cali 760032, ColombiaGrupo de Materiales Compuestos, Escuela de Ingeniería de Materiales, Facultad de Ingeniería, Universidad del Valle, Calle 13 No. 100-00, Cali 760032, ColombiaGrupo de Investigación de Fotoquímica y Fotobiología, Universidad del Atlántico, Carrera 30 Número 8-49, Puerto Colombia 081008, ColombiaThis research focused on developing new materials for endodontic treatments to restore tissues affected by infectious or inflammatory processes. Three materials were studied, namely tricalcium phosphate β-hydroxyapatite (β-TCP), commercial and natural hydroxyapatite (HA), and chitosan (CS), in different proportions. The chemical characterization using infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis confirmed the composition of the composite. Scanning electron microscopy (SEM) demonstrated that the design and origin of the HA, whether natural or commercial, did not affect the morphology of the composites. In vitro studies using <i>Artemia salina</i> (<i>A. salina</i>) indicated that all three experimental materials were biocompatible after 24 h, with no significant differences in mortality rate observed among the groups. The subdermal implantation of the materials in block form exhibited biocompatibility and biodegradability after 30 and 60 days, with the larger particles undergoing fragmentation and connective tissue formation consisting of collagen type III fibers, blood vessels, and inflammatory cells. The implanted material continued to undergo resorption during this process. The results obtained in this research contribute to developing endodontic technologies for tissue recovery and regeneration.https://www.mdpi.com/2073-4360/15/15/3213<i>Artemia salina</i>biocompatibilitybiodegradabilityendodontic treatment materials |
| spellingShingle | Ingrid Zamora Gilbert Alfonso Morales Jorge Iván Castro Lina Marcela Ruiz Rojas Carlos Humberto Valencia-Llano Jose Herminsul Mina Hernandez Mayra Eliana Valencia Zapata Carlos David Grande-Tovar Chitosan (CS)/Hydroxyapatite (HA)/Tricalcium Phosphate (β-TCP)-Based Composites as a Potential Material for Pulp Tissue Regeneration Polymers <i>Artemia salina</i> biocompatibility biodegradability endodontic treatment materials |
| title | Chitosan (CS)/Hydroxyapatite (HA)/Tricalcium Phosphate (β-TCP)-Based Composites as a Potential Material for Pulp Tissue Regeneration |
| title_full | Chitosan (CS)/Hydroxyapatite (HA)/Tricalcium Phosphate (β-TCP)-Based Composites as a Potential Material for Pulp Tissue Regeneration |
| title_fullStr | Chitosan (CS)/Hydroxyapatite (HA)/Tricalcium Phosphate (β-TCP)-Based Composites as a Potential Material for Pulp Tissue Regeneration |
| title_full_unstemmed | Chitosan (CS)/Hydroxyapatite (HA)/Tricalcium Phosphate (β-TCP)-Based Composites as a Potential Material for Pulp Tissue Regeneration |
| title_short | Chitosan (CS)/Hydroxyapatite (HA)/Tricalcium Phosphate (β-TCP)-Based Composites as a Potential Material for Pulp Tissue Regeneration |
| title_sort | chitosan cs hydroxyapatite ha tricalcium phosphate β tcp based composites as a potential material for pulp tissue regeneration |
| topic | <i>Artemia salina</i> biocompatibility biodegradability endodontic treatment materials |
| url | https://www.mdpi.com/2073-4360/15/15/3213 |
| work_keys_str_mv | AT ingridzamora chitosancshydroxyapatitehatricalciumphosphatebtcpbasedcompositesasapotentialmaterialforpulptissueregeneration AT gilbertalfonsomorales chitosancshydroxyapatitehatricalciumphosphatebtcpbasedcompositesasapotentialmaterialforpulptissueregeneration AT jorgeivancastro chitosancshydroxyapatitehatricalciumphosphatebtcpbasedcompositesasapotentialmaterialforpulptissueregeneration AT linamarcelaruizrojas chitosancshydroxyapatitehatricalciumphosphatebtcpbasedcompositesasapotentialmaterialforpulptissueregeneration AT carloshumbertovalenciallano chitosancshydroxyapatitehatricalciumphosphatebtcpbasedcompositesasapotentialmaterialforpulptissueregeneration AT joseherminsulminahernandez chitosancshydroxyapatitehatricalciumphosphatebtcpbasedcompositesasapotentialmaterialforpulptissueregeneration AT mayraelianavalenciazapata chitosancshydroxyapatitehatricalciumphosphatebtcpbasedcompositesasapotentialmaterialforpulptissueregeneration AT carlosdavidgrandetovar chitosancshydroxyapatitehatricalciumphosphatebtcpbasedcompositesasapotentialmaterialforpulptissueregeneration |