BMSCs-Seeded Interpenetrating Network GelMA/SF Composite Hydrogel for Articular Cartilage Repair
Because of limited self-healing ability, the treatment of articular cartilage defects is still an important clinical challenge. Hydrogel-based biomaterials have broad application prospects in articular cartilage repair. In this study, gelatin methacrylate (GelMA)and silk fibroin (SF) were combined t...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/14/1/39 |
| _version_ | 1797440472624398336 |
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| author | Kaiwen Zheng Xu Zheng Mingzhao Yu Yu He Di Wu |
| author_facet | Kaiwen Zheng Xu Zheng Mingzhao Yu Yu He Di Wu |
| author_sort | Kaiwen Zheng |
| collection | DOAJ |
| description | Because of limited self-healing ability, the treatment of articular cartilage defects is still an important clinical challenge. Hydrogel-based biomaterials have broad application prospects in articular cartilage repair. In this study, gelatin methacrylate (GelMA)and silk fibroin (SF) were combined to form a composite hydrogel with an interpenetrating network (IPN) structure under ultraviolet irradiation and ethanol treatment. Introducing silk fibroin into GelMA hydrogel significantly increased mechanical strength as compressive modulus reached 300 kPa in a GelMA/SF-5 (50 mg/mL silk fibroin) group. Moreover, composite IPN hydrogels demonstrated reduced swelling ratios and favorable biocompatibility and supported chondrogenesis of bone mesenchymal stem cells (BMSCs) at day 7 and day 14. Additionally, significantly higher gene expressions of <i>Col-2</i>, <i>Acan</i>, and <i>Sox-9</i> (<i>p</i> < 0.01) were found in IPN hydrogel groups when compared with the GelMA group. An in vivo study was performed to confirm that the GelMA-SF IPN hydrogel could promote cartilage regeneration. The results showed partial regeneration of cartilage in groups treated with hydrogels only and satisfactory cartilage repair in groups of cell-seeded hydrogels, indicating the necessity of additional seeding cells in hydro-gel-based cartilage treatment. Therefore, our results suggest that the GelMA/SF IPN hydrogels may be a potential functional material in cartilage repair and regeneration. |
| first_indexed | 2024-03-09T12:08:41Z |
| format | Article |
| id | doaj.art-6ef5b0a75a7648d3a2279840ee26839e |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-09T12:08:41Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-6ef5b0a75a7648d3a2279840ee26839e2023-11-30T22:55:10ZengMDPI AGJournal of Functional Biomaterials2079-49832023-01-011413910.3390/jfb14010039BMSCs-Seeded Interpenetrating Network GelMA/SF Composite Hydrogel for Articular Cartilage RepairKaiwen Zheng0Xu Zheng1Mingzhao Yu2Yu He3Di Wu4Department of Orthopaedic Surgery, Shanghai Sixth People’s Hospital, No.600 Yishan Road, Shanghai 200233, ChinaDepartment of Orthopaedic Surgery, Shanghai Sixth People’s Hospital, No.600 Yishan Road, Shanghai 200233, ChinaDepartment of Orthopaedic Surgery, Shanghai Sixth People’s Hospital, No.600 Yishan Road, Shanghai 200233, ChinaDepartment of Plastic Surgery, Plastic Surgery Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100144, ChinaDepartment of Orthopaedic Surgery, Shanghai Sixth People’s Hospital, No.600 Yishan Road, Shanghai 200233, ChinaBecause of limited self-healing ability, the treatment of articular cartilage defects is still an important clinical challenge. Hydrogel-based biomaterials have broad application prospects in articular cartilage repair. In this study, gelatin methacrylate (GelMA)and silk fibroin (SF) were combined to form a composite hydrogel with an interpenetrating network (IPN) structure under ultraviolet irradiation and ethanol treatment. Introducing silk fibroin into GelMA hydrogel significantly increased mechanical strength as compressive modulus reached 300 kPa in a GelMA/SF-5 (50 mg/mL silk fibroin) group. Moreover, composite IPN hydrogels demonstrated reduced swelling ratios and favorable biocompatibility and supported chondrogenesis of bone mesenchymal stem cells (BMSCs) at day 7 and day 14. Additionally, significantly higher gene expressions of <i>Col-2</i>, <i>Acan</i>, and <i>Sox-9</i> (<i>p</i> < 0.01) were found in IPN hydrogel groups when compared with the GelMA group. An in vivo study was performed to confirm that the GelMA-SF IPN hydrogel could promote cartilage regeneration. The results showed partial regeneration of cartilage in groups treated with hydrogels only and satisfactory cartilage repair in groups of cell-seeded hydrogels, indicating the necessity of additional seeding cells in hydro-gel-based cartilage treatment. Therefore, our results suggest that the GelMA/SF IPN hydrogels may be a potential functional material in cartilage repair and regeneration.https://www.mdpi.com/2079-4983/14/1/39hydrogelinterpenetrated networkarticular cartilageosteochondral defect |
| spellingShingle | Kaiwen Zheng Xu Zheng Mingzhao Yu Yu He Di Wu BMSCs-Seeded Interpenetrating Network GelMA/SF Composite Hydrogel for Articular Cartilage Repair Journal of Functional Biomaterials hydrogel interpenetrated network articular cartilage osteochondral defect |
| title | BMSCs-Seeded Interpenetrating Network GelMA/SF Composite Hydrogel for Articular Cartilage Repair |
| title_full | BMSCs-Seeded Interpenetrating Network GelMA/SF Composite Hydrogel for Articular Cartilage Repair |
| title_fullStr | BMSCs-Seeded Interpenetrating Network GelMA/SF Composite Hydrogel for Articular Cartilage Repair |
| title_full_unstemmed | BMSCs-Seeded Interpenetrating Network GelMA/SF Composite Hydrogel for Articular Cartilage Repair |
| title_short | BMSCs-Seeded Interpenetrating Network GelMA/SF Composite Hydrogel for Articular Cartilage Repair |
| title_sort | bmscs seeded interpenetrating network gelma sf composite hydrogel for articular cartilage repair |
| topic | hydrogel interpenetrated network articular cartilage osteochondral defect |
| url | https://www.mdpi.com/2079-4983/14/1/39 |
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