Synthesis of Phospholipid-Protein Conjugates as New Antigens for Autoimmune Antibodies
Copper(I)-catalyzed azide-alkyne cycloaddition, or CuAAC click chemistry, is an efficient method for bioconjugation aiming at chemical and biological applications. Herein, we demonstrate how the CuAAC method can provide novel phospholipid-protein conjugates with a high potential for the diagnostics...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2015-06-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/20/6/10253 |
| _version_ | 1819181900375261184 |
|---|---|
| author | Arindam Maity Claudia Macaubas Elizabeth Mellins Kira Astakhova |
| author_facet | Arindam Maity Claudia Macaubas Elizabeth Mellins Kira Astakhova |
| author_sort | Arindam Maity |
| collection | DOAJ |
| description | Copper(I)-catalyzed azide-alkyne cycloaddition, or CuAAC click chemistry, is an efficient method for bioconjugation aiming at chemical and biological applications. Herein, we demonstrate how the CuAAC method can provide novel phospholipid-protein conjugates with a high potential for the diagnostics and therapy of autoimmune conditions. In doing this, we, for the first time, covalently bind via 1,2,3-triazole linker biologically complementary molecules, namely phosphoethanol amine with human β2-glycoprotein I and prothrombin. The resulting phospholipid-protein conjugates show high binding affinity and specificity for the autoimmune antibodies against autoimmune complexes. Thus, the development of this work might become a milestone in further diagnostics and therapy of autoimmune diseases that involve the production of autoantibodies against the aforementioned phospholipids and proteins, such as antiphospholipid syndrome and systemic lupus erythematosus. |
| first_indexed | 2024-12-22T22:37:35Z |
| format | Article |
| id | doaj.art-6efdfcddc96a45548abe5a537d157afc |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-22T22:37:35Z |
| publishDate | 2015-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-6efdfcddc96a45548abe5a537d157afc2022-12-21T18:10:16ZengMDPI AGMolecules1420-30492015-06-01206102531026310.3390/molecules200610253molecules200610253Synthesis of Phospholipid-Protein Conjugates as New Antigens for Autoimmune AntibodiesArindam Maity0Claudia Macaubas1Elizabeth Mellins2Kira Astakhova3Nucleic Acid Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Campusvej 55, Odense 5230, DenmarkDivisions of Human Gene Therapy and Pediatric Rheumatology, Program in Immunology, Stanford University School of Medicine, 269 Campus Drive, Stanford, MC 5164, USADivisions of Human Gene Therapy and Pediatric Rheumatology, Program in Immunology, Stanford University School of Medicine, 269 Campus Drive, Stanford, MC 5164, USANucleic Acid Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Campusvej 55, Odense 5230, DenmarkCopper(I)-catalyzed azide-alkyne cycloaddition, or CuAAC click chemistry, is an efficient method for bioconjugation aiming at chemical and biological applications. Herein, we demonstrate how the CuAAC method can provide novel phospholipid-protein conjugates with a high potential for the diagnostics and therapy of autoimmune conditions. In doing this, we, for the first time, covalently bind via 1,2,3-triazole linker biologically complementary molecules, namely phosphoethanol amine with human β2-glycoprotein I and prothrombin. The resulting phospholipid-protein conjugates show high binding affinity and specificity for the autoimmune antibodies against autoimmune complexes. Thus, the development of this work might become a milestone in further diagnostics and therapy of autoimmune diseases that involve the production of autoantibodies against the aforementioned phospholipids and proteins, such as antiphospholipid syndrome and systemic lupus erythematosus.http://www.mdpi.com/1420-3049/20/6/10253CuAAC click chemistryantiphospholipid syndromeantigensβ2-glycoprotein Iphosphoethanolamineprothrombin |
| spellingShingle | Arindam Maity Claudia Macaubas Elizabeth Mellins Kira Astakhova Synthesis of Phospholipid-Protein Conjugates as New Antigens for Autoimmune Antibodies Molecules CuAAC click chemistry antiphospholipid syndrome antigens β2-glycoprotein I phosphoethanolamine prothrombin |
| title | Synthesis of Phospholipid-Protein Conjugates as New Antigens for Autoimmune Antibodies |
| title_full | Synthesis of Phospholipid-Protein Conjugates as New Antigens for Autoimmune Antibodies |
| title_fullStr | Synthesis of Phospholipid-Protein Conjugates as New Antigens for Autoimmune Antibodies |
| title_full_unstemmed | Synthesis of Phospholipid-Protein Conjugates as New Antigens for Autoimmune Antibodies |
| title_short | Synthesis of Phospholipid-Protein Conjugates as New Antigens for Autoimmune Antibodies |
| title_sort | synthesis of phospholipid protein conjugates as new antigens for autoimmune antibodies |
| topic | CuAAC click chemistry antiphospholipid syndrome antigens β2-glycoprotein I phosphoethanolamine prothrombin |
| url | http://www.mdpi.com/1420-3049/20/6/10253 |
| work_keys_str_mv | AT arindammaity synthesisofphospholipidproteinconjugatesasnewantigensforautoimmuneantibodies AT claudiamacaubas synthesisofphospholipidproteinconjugatesasnewantigensforautoimmuneantibodies AT elizabethmellins synthesisofphospholipidproteinconjugatesasnewantigensforautoimmuneantibodies AT kiraastakhova synthesisofphospholipidproteinconjugatesasnewantigensforautoimmuneantibodies |