pWCP is a widely distributed and highly conserved Wolbachia plasmid in Culex pipiens and Culex quinquefasciatus mosquitoes worldwide
Abstract Mosquitoes represent the most important pathogen vectors and are responsible for the spread of a wide variety of poorly treatable diseases. Wolbachia are obligate intracellular bacteria that are widely distributed among arthropods and collectively represents one of the most promising soluti...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Oxford University Press
2023-04-01
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| Series: | ISME Communications |
| Online Access: | https://doi.org/10.1038/s43705-023-00248-2 |
| _version_ | 1827297767471448064 |
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| author | Amani Ghousein Jordan Tutagata Hans Schrieke Manuel Etienne Victor Chaumeau Sebastien Boyer Nonito Pages David Roiz A. Murat Eren Guillaume Cambray Julie Reveillaud |
| author_facet | Amani Ghousein Jordan Tutagata Hans Schrieke Manuel Etienne Victor Chaumeau Sebastien Boyer Nonito Pages David Roiz A. Murat Eren Guillaume Cambray Julie Reveillaud |
| author_sort | Amani Ghousein |
| collection | DOAJ |
| description | Abstract Mosquitoes represent the most important pathogen vectors and are responsible for the spread of a wide variety of poorly treatable diseases. Wolbachia are obligate intracellular bacteria that are widely distributed among arthropods and collectively represents one of the most promising solutions for vector control. In particular, Wolbachia has been shown to limit the transmission of pathogens, and to dramatically affect the reproductive behavior of their host through its phage WO. While much research has focused on deciphering and exploring the biocontrol applications of these WO-related phenotypes, the extent and potential impact of the Wolbachia mobilome remain poorly appreciated. Notably, several Wolbachia plasmids, carrying WO-like genes and Insertion Sequences (IS), thus possibly interrelated to other genetic units of the endosymbiont, have been recently discovered. Here we investigated the diversity and biogeography of the first described plasmid of Wolbachia in Culex pipiens (pWCP) in several islands and continental countries around the world—including Cambodia, Guadeloupe, Martinique, Thailand, and Mexico—together with mosquito strains from colonies that evolved for 2 to 30 years in the laboratory. We used PCR and qPCR to determine the presence and copy number of pWCP in individual mosquitoes, and highly accurate Sanger sequencing to evaluate potential variations. Together with earlier observation, our results show that pWCP is omnipresent and strikingly conserved among Wolbachia populations within mosquitoes from distant geographies and environmental conditions. These data suggest a critical role for the plasmid in Wolbachia ecology and evolution, and the potential of a great tool for further genetic dissection and possible manipulation of this endosymbiont. |
| first_indexed | 2024-04-24T15:02:04Z |
| format | Article |
| id | doaj.art-6efe14fa143e4e4aa603d61f2bb99f8c |
| institution | Directory Open Access Journal |
| issn | 2730-6151 |
| language | English |
| last_indexed | 2024-04-24T15:02:04Z |
| publishDate | 2023-04-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | ISME Communications |
| spelling | doaj.art-6efe14fa143e4e4aa603d61f2bb99f8c2024-04-02T14:42:16ZengOxford University PressISME Communications2730-61512023-04-01311510.1038/s43705-023-00248-2pWCP is a widely distributed and highly conserved Wolbachia plasmid in Culex pipiens and Culex quinquefasciatus mosquitoes worldwideAmani Ghousein0Jordan Tutagata1Hans Schrieke2Manuel Etienne3Victor Chaumeau4Sebastien Boyer5Nonito Pages6David Roiz7A. Murat Eren8Guillaume Cambray9Julie Reveillaud10MIVEGEC, University of Montpellier, INRAE, CNRS, IRDMIVEGEC, University of Montpellier, INRAE, CNRS, IRDMIVEGEC, University of Montpellier, INRAE, CNRS, IRDCentre de Démoustication et de Recherches Entomologiques - Lutte Anti-Vectorielle (CEDRE - LAV), avenue PasteurShoklo Malaria Research Unit, Mahidol-Oxford Research Unit, Faculty of Tropical Medicine, Mahidol UniversityInstitut Pasteur du Cambodge, Medical Entomology UnitASTRE, University of Montpellier, CIRAD, INRAEMIVEGEC, University of Montpellier, INRAE, CNRS, IRDMarine Biological Laboratory, Woods HoleCentre de Biologie Structurale (CBS), University of Montpellier, INSERM U1054MIVEGEC, University of Montpellier, INRAE, CNRS, IRDAbstract Mosquitoes represent the most important pathogen vectors and are responsible for the spread of a wide variety of poorly treatable diseases. Wolbachia are obligate intracellular bacteria that are widely distributed among arthropods and collectively represents one of the most promising solutions for vector control. In particular, Wolbachia has been shown to limit the transmission of pathogens, and to dramatically affect the reproductive behavior of their host through its phage WO. While much research has focused on deciphering and exploring the biocontrol applications of these WO-related phenotypes, the extent and potential impact of the Wolbachia mobilome remain poorly appreciated. Notably, several Wolbachia plasmids, carrying WO-like genes and Insertion Sequences (IS), thus possibly interrelated to other genetic units of the endosymbiont, have been recently discovered. Here we investigated the diversity and biogeography of the first described plasmid of Wolbachia in Culex pipiens (pWCP) in several islands and continental countries around the world—including Cambodia, Guadeloupe, Martinique, Thailand, and Mexico—together with mosquito strains from colonies that evolved for 2 to 30 years in the laboratory. We used PCR and qPCR to determine the presence and copy number of pWCP in individual mosquitoes, and highly accurate Sanger sequencing to evaluate potential variations. Together with earlier observation, our results show that pWCP is omnipresent and strikingly conserved among Wolbachia populations within mosquitoes from distant geographies and environmental conditions. These data suggest a critical role for the plasmid in Wolbachia ecology and evolution, and the potential of a great tool for further genetic dissection and possible manipulation of this endosymbiont.https://doi.org/10.1038/s43705-023-00248-2 |
| spellingShingle | Amani Ghousein Jordan Tutagata Hans Schrieke Manuel Etienne Victor Chaumeau Sebastien Boyer Nonito Pages David Roiz A. Murat Eren Guillaume Cambray Julie Reveillaud pWCP is a widely distributed and highly conserved Wolbachia plasmid in Culex pipiens and Culex quinquefasciatus mosquitoes worldwide ISME Communications |
| title | pWCP is a widely distributed and highly conserved Wolbachia plasmid in Culex pipiens and Culex quinquefasciatus mosquitoes worldwide |
| title_full | pWCP is a widely distributed and highly conserved Wolbachia plasmid in Culex pipiens and Culex quinquefasciatus mosquitoes worldwide |
| title_fullStr | pWCP is a widely distributed and highly conserved Wolbachia plasmid in Culex pipiens and Culex quinquefasciatus mosquitoes worldwide |
| title_full_unstemmed | pWCP is a widely distributed and highly conserved Wolbachia plasmid in Culex pipiens and Culex quinquefasciatus mosquitoes worldwide |
| title_short | pWCP is a widely distributed and highly conserved Wolbachia plasmid in Culex pipiens and Culex quinquefasciatus mosquitoes worldwide |
| title_sort | pwcp is a widely distributed and highly conserved wolbachia plasmid in culex pipiens and culex quinquefasciatus mosquitoes worldwide |
| url | https://doi.org/10.1038/s43705-023-00248-2 |
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