A Structural View of miRNA Biogenesis and Function
Micro-RNAs (miRNAs) are a class of non-coding RNAs (ncRNAs) that act as post-transcriptional regulators of gene expression. Since their discovery in 1993, they have been the subject of deep study due to their involvement in many important biological processes. Compared with other ncRNAs, miRNAs are...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Non-Coding RNA |
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| Online Access: | https://www.mdpi.com/2311-553X/8/1/10 |
| _version_ | 1797477524685455360 |
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| author | Ana Lúcia Leitão Francisco J. Enguita |
| author_facet | Ana Lúcia Leitão Francisco J. Enguita |
| author_sort | Ana Lúcia Leitão |
| collection | DOAJ |
| description | Micro-RNAs (miRNAs) are a class of non-coding RNAs (ncRNAs) that act as post-transcriptional regulators of gene expression. Since their discovery in 1993, they have been the subject of deep study due to their involvement in many important biological processes. Compared with other ncRNAs, miRNAs are generated from devoted transcriptional units which are processed by a specific set of endonucleases. The contribution of structural biology methods for understanding miRNA biogenesis and function has been essential for the dissection of their roles in cell biology and human disease. In this review, we summarize the application of structural biology for the characterization of the molecular players involved in miRNA biogenesis (processors and effectors), starting from the X-ray crystallography methods to the more recent cryo-electron microscopy protocols. |
| first_indexed | 2024-03-09T21:18:54Z |
| format | Article |
| id | doaj.art-6f02b1437251490882e61e8a644ecf2c |
| institution | Directory Open Access Journal |
| issn | 2311-553X |
| language | English |
| last_indexed | 2024-03-09T21:18:54Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Non-Coding RNA |
| spelling | doaj.art-6f02b1437251490882e61e8a644ecf2c2023-11-23T21:27:14ZengMDPI AGNon-Coding RNA2311-553X2022-01-01811010.3390/ncrna8010010A Structural View of miRNA Biogenesis and FunctionAna Lúcia Leitão0Francisco J. Enguita1MEtRICs, Department of Sciences and Technology of Biomass, NOVA School of Science and Technology, FCT NOVA, Universidade NOVA de Lisboa, 2829-516 Caparica, PortugalInstituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Av. Prof. Egas Moniz, 1649-028 Lisboa, PortugalMicro-RNAs (miRNAs) are a class of non-coding RNAs (ncRNAs) that act as post-transcriptional regulators of gene expression. Since their discovery in 1993, they have been the subject of deep study due to their involvement in many important biological processes. Compared with other ncRNAs, miRNAs are generated from devoted transcriptional units which are processed by a specific set of endonucleases. The contribution of structural biology methods for understanding miRNA biogenesis and function has been essential for the dissection of their roles in cell biology and human disease. In this review, we summarize the application of structural biology for the characterization of the molecular players involved in miRNA biogenesis (processors and effectors), starting from the X-ray crystallography methods to the more recent cryo-electron microscopy protocols.https://www.mdpi.com/2311-553X/8/1/10microRNAbiogenesisstructural biologyX-ray crystallographycryo-electron microscopynuclease |
| spellingShingle | Ana Lúcia Leitão Francisco J. Enguita A Structural View of miRNA Biogenesis and Function Non-Coding RNA microRNA biogenesis structural biology X-ray crystallography cryo-electron microscopy nuclease |
| title | A Structural View of miRNA Biogenesis and Function |
| title_full | A Structural View of miRNA Biogenesis and Function |
| title_fullStr | A Structural View of miRNA Biogenesis and Function |
| title_full_unstemmed | A Structural View of miRNA Biogenesis and Function |
| title_short | A Structural View of miRNA Biogenesis and Function |
| title_sort | structural view of mirna biogenesis and function |
| topic | microRNA biogenesis structural biology X-ray crystallography cryo-electron microscopy nuclease |
| url | https://www.mdpi.com/2311-553X/8/1/10 |
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