Magnetic Resonance Tracking of Human CD34 Progenitor Cells Separated by Means of Immunomagnetic Selection and Transplanted into Injured Rat Brain
Magnetic resonance imaging (MRI) provides a noninvasive method for studying the fate of transplanted cells in vivo. We studied whether superparamagnetic nanoparticles (CD34 microbeads), used clinically for specific magnetic sorting, can be used as a magnetic cell label for in vivo cell visualization...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2005-04-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/000000005783983124 |
| _version_ | 1818302777283051520 |
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| author | Pavla Jendelová Ph.D. Vít Herynek Lucia Urdziková KateřIna Glogarová Šárka Rahmatová Ivan Fales Benita Andersson Pavel Procházka Josef Zamečník Tomáš Eckschlager Petr Kobylka Milan Hájek Eva Sykova |
| author_facet | Pavla Jendelová Ph.D. Vít Herynek Lucia Urdziková KateřIna Glogarová Šárka Rahmatová Ivan Fales Benita Andersson Pavel Procházka Josef Zamečník Tomáš Eckschlager Petr Kobylka Milan Hájek Eva Sykova |
| author_sort | Pavla Jendelová Ph.D. |
| collection | DOAJ |
| description | Magnetic resonance imaging (MRI) provides a noninvasive method for studying the fate of transplanted cells in vivo. We studied whether superparamagnetic nanoparticles (CD34 microbeads), used clinically for specific magnetic sorting, can be used as a magnetic cell label for in vivo cell visualization. Human cells from peripheral blood were selected by CliniMACS® CD34 Selection Technology (Miltenyi). Purified CD34+ cells were implanted into rats with a cortical photochemical lesion, contralaterally to the lesion. Twenty-four hours after grafting, the implanted cells were detected in the contralateral hemisphere as a hypointense spot on T 2 weighted images; the hypointensity of the implant decreased during the first week. At the lesion site we observed a hypointensive signal 10 days after grafting that persisted for the next 3 weeks, until the end of the experiment. Prussian blue and anti-human nuclei staining confirmed the presence of magnetically labeled human cells in the corpus callosum and in the lesion 4 weeks after grafting. CD34+ cells were also found in the subventricular zone (SVZ). Human DNA (a human-specific 850 base pair fragment of α-satellite DNA from human chromosome 17) was detected in brain tissue sections from the lesion using PCR, confirming the presence of human cells. Our results show that CD34 microbeads superparamagnetic nanoparticles can be used as a magnetic cell label for in vivo cell visualization. The fact that microbeads coated with different commercially available antibodies can bind to specific cell types opens extensive possibilities for cell tracking in vivo. |
| first_indexed | 2024-12-13T05:44:18Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-13T05:44:18Z |
| publishDate | 2005-04-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-6f048027ffcd445d8ee1662adedabde02022-12-21T23:57:42ZengSAGE PublishingCell Transplantation0963-68971555-38922005-04-011410.3727/000000005783983124Magnetic Resonance Tracking of Human CD34 Progenitor Cells Separated by Means of Immunomagnetic Selection and Transplanted into Injured Rat BrainPavla Jendelová Ph.D.0Vít Herynek1Lucia Urdziková2KateřIna Glogarová3Šárka Rahmatová4Ivan Fales5Benita Andersson6Pavel Procházka7Josef Zamečník8Tomáš Eckschlager9Petr Kobylka10Milan Hájek11Eva Sykova12Institute of Experimental Medicine ASCR, Prague, Czech RepublicMR-Unit, Department of Diagnostic and Interventional Radiology, Institute for Clinical and Experimental Medicine, Prague, Czech RepublicInstitute of Experimental Medicine ASCR, Prague, Czech RepublicCenter for Cell Therapy and Tissue Repair, Charles University, Prague, Czech RepublicInstitute of Hematology and Blood Transfusion, Prague, Czech RepublicInstitute of Hematology and Blood Transfusion, Prague, Czech RepublicInstitute of Experimental Medicine ASCR, Prague, Czech RepublicDepartment of Paediatric Haematology and Oncology, Charles University, Second Medical Faculty, Prague, Czech RepublicDepartment of Pathology and Molecular Medicine, Charles University, Second Medical Faculty, Prague, Czech RepublicDepartment of Paediatric Haematology and Oncology, Charles University, Second Medical Faculty, Prague, Czech RepublicInstitute of Hematology and Blood Transfusion, Prague, Czech RepublicMR-Unit, Department of Diagnostic and Interventional Radiology, Institute for Clinical and Experimental Medicine, Prague, Czech RepublicDepartment of Neuroscience, Charles University, Second Medical Faculty, Prague, Czech RepublicMagnetic resonance imaging (MRI) provides a noninvasive method for studying the fate of transplanted cells in vivo. We studied whether superparamagnetic nanoparticles (CD34 microbeads), used clinically for specific magnetic sorting, can be used as a magnetic cell label for in vivo cell visualization. Human cells from peripheral blood were selected by CliniMACS® CD34 Selection Technology (Miltenyi). Purified CD34+ cells were implanted into rats with a cortical photochemical lesion, contralaterally to the lesion. Twenty-four hours after grafting, the implanted cells were detected in the contralateral hemisphere as a hypointense spot on T 2 weighted images; the hypointensity of the implant decreased during the first week. At the lesion site we observed a hypointensive signal 10 days after grafting that persisted for the next 3 weeks, until the end of the experiment. Prussian blue and anti-human nuclei staining confirmed the presence of magnetically labeled human cells in the corpus callosum and in the lesion 4 weeks after grafting. CD34+ cells were also found in the subventricular zone (SVZ). Human DNA (a human-specific 850 base pair fragment of α-satellite DNA from human chromosome 17) was detected in brain tissue sections from the lesion using PCR, confirming the presence of human cells. Our results show that CD34 microbeads superparamagnetic nanoparticles can be used as a magnetic cell label for in vivo cell visualization. The fact that microbeads coated with different commercially available antibodies can bind to specific cell types opens extensive possibilities for cell tracking in vivo.https://doi.org/10.3727/000000005783983124 |
| spellingShingle | Pavla Jendelová Ph.D. Vít Herynek Lucia Urdziková KateřIna Glogarová Šárka Rahmatová Ivan Fales Benita Andersson Pavel Procházka Josef Zamečník Tomáš Eckschlager Petr Kobylka Milan Hájek Eva Sykova Magnetic Resonance Tracking of Human CD34 Progenitor Cells Separated by Means of Immunomagnetic Selection and Transplanted into Injured Rat Brain Cell Transplantation |
| title | Magnetic Resonance Tracking of Human CD34 Progenitor Cells Separated by Means of Immunomagnetic Selection and Transplanted into Injured Rat Brain |
| title_full | Magnetic Resonance Tracking of Human CD34 Progenitor Cells Separated by Means of Immunomagnetic Selection and Transplanted into Injured Rat Brain |
| title_fullStr | Magnetic Resonance Tracking of Human CD34 Progenitor Cells Separated by Means of Immunomagnetic Selection and Transplanted into Injured Rat Brain |
| title_full_unstemmed | Magnetic Resonance Tracking of Human CD34 Progenitor Cells Separated by Means of Immunomagnetic Selection and Transplanted into Injured Rat Brain |
| title_short | Magnetic Resonance Tracking of Human CD34 Progenitor Cells Separated by Means of Immunomagnetic Selection and Transplanted into Injured Rat Brain |
| title_sort | magnetic resonance tracking of human cd34 progenitor cells separated by means of immunomagnetic selection and transplanted into injured rat brain |
| url | https://doi.org/10.3727/000000005783983124 |
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