Isolation of nuclei from frozen human subcutaneous adipose tissue for full-length single-nuclei transcriptional profiling

Isolation of nuclei from frozen human subcutaneous adipose tissue for full-length single-nuclei transcriptional profiling

Summary: Automated single-cell dispensing is incompatible with white adipose tissue (WAT) due to lipid-laden adipocytes. Single-nuclei RNA-Seq permits transcriptional profiling of all cells from WAT. Human WAT faces unique technical challenges in isolating nuclei compared to rodent tissue due to gre...

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Bibliographic Details
Main Authors: Katie L. Whytock, Adeline Divoux, Yifei Sun, Meghan Hopf, Reichelle X. Yeo, Maria F. Pino, GongXin Yu, Steven R. Smith, Martin J. Walsh, Lauren M. Sparks
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:STAR Protocols
Subjects:
Cell isolation
Single Cell
RNAseq
Metabolism
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723000126

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