LncRNA LINC00342 promotes gastric cancer progression by targeting the miR-545-5p/CNPY2 axis
Abstract Background This study aimed to explore the role and underlying molecular mechanisms of long non-coding RNA (lncRNA) LINC00342 in gastric cancer (GC). Methods The expression of LINC00342 in GC tissues was evaluated by Quantitative reverse transcription polymerase chain reaction (qRT-PCR). Si...
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| Format: | Article |
| Language: | English |
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BMC
2021-10-01
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| Series: | BMC Cancer |
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| Online Access: | https://doi.org/10.1186/s12885-021-08829-x |
| _version_ | 1819009231339126784 |
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| author | Run Liu Xianwu Yang |
| author_facet | Run Liu Xianwu Yang |
| author_sort | Run Liu |
| collection | DOAJ |
| description | Abstract Background This study aimed to explore the role and underlying molecular mechanisms of long non-coding RNA (lncRNA) LINC00342 in gastric cancer (GC). Methods The expression of LINC00342 in GC tissues was evaluated by Quantitative reverse transcription polymerase chain reaction (qRT-PCR). Silencing of LINC00342 was conducted to investigate the effect of LINC00342 in vitro and in vivo. The underlying molecular mechanisms of LINC00342 were determined by dual luciferase reporter assay, Western blotting analysis and rescue experiments. Biological functions of LINC00342 were evaluated by cell counting kit-8 (CCK-8) assay, colony formation assay, wound healing assay and Transwell assays. In addition, a tumor model was used to verify the effect of LINC00342 in tumorigenesis in vivo. Results LINC00342 was significantly upregulated in GC tissues and cell lines. Silencing of LINC00342 efficiently inhibited proliferation, migration and invasion of AGS cells in vitro, and also suppressed the tumorigenesis of GC in vivo. Functional experiments showed that LINC00342 regulated the expression of canopy fibroblast growth factor signaling regulator 2 (CNPY2) by competitively sponging miR-545-5p. Rescue experiments showed that inhibition of miR-545-5p and overexpression of CNPY2 significantly reversed cell phenotypes caused by silencing of LINC00342. Conclusion LINC00342 plays a potential oncogenic role in GC by targeting the miR545-5p/CNPY2 axis, and might act as a novel therapeutic target for GC. |
| first_indexed | 2024-12-21T00:53:05Z |
| format | Article |
| id | doaj.art-6f07ba7f396a4ef89e27a5ec52b1f2aa |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-21T00:53:05Z |
| publishDate | 2021-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-6f07ba7f396a4ef89e27a5ec52b1f2aa2022-12-21T19:21:22ZengBMCBMC Cancer1471-24072021-10-0121111210.1186/s12885-021-08829-xLncRNA LINC00342 promotes gastric cancer progression by targeting the miR-545-5p/CNPY2 axisRun Liu0Xianwu Yang1Department of Gastroenterology, The Shijiazhuang People’s HospitalDepartment of Gastroenterology, The Shijiazhuang People’s HospitalAbstract Background This study aimed to explore the role and underlying molecular mechanisms of long non-coding RNA (lncRNA) LINC00342 in gastric cancer (GC). Methods The expression of LINC00342 in GC tissues was evaluated by Quantitative reverse transcription polymerase chain reaction (qRT-PCR). Silencing of LINC00342 was conducted to investigate the effect of LINC00342 in vitro and in vivo. The underlying molecular mechanisms of LINC00342 were determined by dual luciferase reporter assay, Western blotting analysis and rescue experiments. Biological functions of LINC00342 were evaluated by cell counting kit-8 (CCK-8) assay, colony formation assay, wound healing assay and Transwell assays. In addition, a tumor model was used to verify the effect of LINC00342 in tumorigenesis in vivo. Results LINC00342 was significantly upregulated in GC tissues and cell lines. Silencing of LINC00342 efficiently inhibited proliferation, migration and invasion of AGS cells in vitro, and also suppressed the tumorigenesis of GC in vivo. Functional experiments showed that LINC00342 regulated the expression of canopy fibroblast growth factor signaling regulator 2 (CNPY2) by competitively sponging miR-545-5p. Rescue experiments showed that inhibition of miR-545-5p and overexpression of CNPY2 significantly reversed cell phenotypes caused by silencing of LINC00342. Conclusion LINC00342 plays a potential oncogenic role in GC by targeting the miR545-5p/CNPY2 axis, and might act as a novel therapeutic target for GC.https://doi.org/10.1186/s12885-021-08829-xGastric cancerLINC00342Tumorigenesis |
| spellingShingle | Run Liu Xianwu Yang LncRNA LINC00342 promotes gastric cancer progression by targeting the miR-545-5p/CNPY2 axis BMC Cancer Gastric cancer LINC00342 Tumorigenesis |
| title | LncRNA LINC00342 promotes gastric cancer progression by targeting the miR-545-5p/CNPY2 axis |
| title_full | LncRNA LINC00342 promotes gastric cancer progression by targeting the miR-545-5p/CNPY2 axis |
| title_fullStr | LncRNA LINC00342 promotes gastric cancer progression by targeting the miR-545-5p/CNPY2 axis |
| title_full_unstemmed | LncRNA LINC00342 promotes gastric cancer progression by targeting the miR-545-5p/CNPY2 axis |
| title_short | LncRNA LINC00342 promotes gastric cancer progression by targeting the miR-545-5p/CNPY2 axis |
| title_sort | lncrna linc00342 promotes gastric cancer progression by targeting the mir 545 5p cnpy2 axis |
| topic | Gastric cancer LINC00342 Tumorigenesis |
| url | https://doi.org/10.1186/s12885-021-08829-x |
| work_keys_str_mv | AT runliu lncrnalinc00342promotesgastriccancerprogressionbytargetingthemir5455pcnpy2axis AT xianwuyang lncrnalinc00342promotesgastriccancerprogressionbytargetingthemir5455pcnpy2axis |