Targeted Molecular Detection of Nosocomial Carbapenemase-Producing Gram-Negative Bacteria—On Near- and Distant-Patient Surfaces
Background: Here, we describe an integrative method to detect carbapenemase-producing Gram-negative bacteria (gn-Cp) on surfaces/fomites in the patient environment. We examined environmental samples from 28 patient rooms occupied with patients who were proven to be colonised with gn-Cp by rectal scr...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/6/1190 |
| _version_ | 1797531860680572928 |
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| author | Claudia Stein Isabel Lange Jürgen Rödel Mathias W. Pletz Frank Kipp |
| author_facet | Claudia Stein Isabel Lange Jürgen Rödel Mathias W. Pletz Frank Kipp |
| author_sort | Claudia Stein |
| collection | DOAJ |
| description | Background: Here, we describe an integrative method to detect carbapenemase-producing Gram-negative bacteria (gn-Cp) on surfaces/fomites in the patient environment. We examined environmental samples from 28 patient rooms occupied with patients who were proven to be colonised with gn-Cp by rectal screening. Methods: We took samples after 24 h, 72 h and one week. For sampling, we divided the patient environment into four parts and took samples from near- and extended patient areas. To obtain a representative bacterial swab from a larger surface, such as the patient cabinet, we used Polywipes. Bacterial DNA was isolated. Carbapenemase was detected with specific qPCR primers. Results: With this culture- and molecular-based approach, we could control the effectiveness of cleaning and disinfection in everyday clinical practice. Therefore, we could track the spread of gn-Cp within the patient room. The number of positive detections fluctuated between 30.5% (mean value positive results after 72 h) and 35.2% (after 24 h and one week). Conclusion: The method used to detect multidrug-resistant bacteria in the environment of patients by using Polywipes<sup>TM</sup> is reliable and can therefore be used as an effective, new tool in hygiene and infection control. |
| first_indexed | 2024-03-10T10:49:46Z |
| format | Article |
| id | doaj.art-6f0ab58bd700466b8ba07bfc5566729d |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T10:49:46Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-6f0ab58bd700466b8ba07bfc5566729d2023-11-21T22:17:56ZengMDPI AGMicroorganisms2076-26072021-05-0196119010.3390/microorganisms9061190Targeted Molecular Detection of Nosocomial Carbapenemase-Producing Gram-Negative Bacteria—On Near- and Distant-Patient SurfacesClaudia Stein0Isabel Lange1Jürgen Rödel2Mathias W. Pletz3Frank Kipp4Institute for Infectious Diseases and Infection Control, Jena University Hospital, Am Klinikum 1, 07747 Jena, GermanyInstitute for Infectious Diseases and Infection Control, Jena University Hospital, Am Klinikum 1, 07747 Jena, GermanyInstitute of Medical Microbiology, Jena University Hospital, Am Klinikum 1, 07747 Jena, GermanyInstitute for Infectious Diseases and Infection Control, Jena University Hospital, Am Klinikum 1, 07747 Jena, GermanyInstitute for Infectious Diseases and Infection Control, Jena University Hospital, Am Klinikum 1, 07747 Jena, GermanyBackground: Here, we describe an integrative method to detect carbapenemase-producing Gram-negative bacteria (gn-Cp) on surfaces/fomites in the patient environment. We examined environmental samples from 28 patient rooms occupied with patients who were proven to be colonised with gn-Cp by rectal screening. Methods: We took samples after 24 h, 72 h and one week. For sampling, we divided the patient environment into four parts and took samples from near- and extended patient areas. To obtain a representative bacterial swab from a larger surface, such as the patient cabinet, we used Polywipes. Bacterial DNA was isolated. Carbapenemase was detected with specific qPCR primers. Results: With this culture- and molecular-based approach, we could control the effectiveness of cleaning and disinfection in everyday clinical practice. Therefore, we could track the spread of gn-Cp within the patient room. The number of positive detections fluctuated between 30.5% (mean value positive results after 72 h) and 35.2% (after 24 h and one week). Conclusion: The method used to detect multidrug-resistant bacteria in the environment of patients by using Polywipes<sup>TM</sup> is reliable and can therefore be used as an effective, new tool in hygiene and infection control.https://www.mdpi.com/2076-2607/9/6/1190molecular detectionfomitescarbapenemase-producing Gram-negative bacteriahygienepatient’s room |
| spellingShingle | Claudia Stein Isabel Lange Jürgen Rödel Mathias W. Pletz Frank Kipp Targeted Molecular Detection of Nosocomial Carbapenemase-Producing Gram-Negative Bacteria—On Near- and Distant-Patient Surfaces Microorganisms molecular detection fomites carbapenemase-producing Gram-negative bacteria hygiene patient’s room |
| title | Targeted Molecular Detection of Nosocomial Carbapenemase-Producing Gram-Negative Bacteria—On Near- and Distant-Patient Surfaces |
| title_full | Targeted Molecular Detection of Nosocomial Carbapenemase-Producing Gram-Negative Bacteria—On Near- and Distant-Patient Surfaces |
| title_fullStr | Targeted Molecular Detection of Nosocomial Carbapenemase-Producing Gram-Negative Bacteria—On Near- and Distant-Patient Surfaces |
| title_full_unstemmed | Targeted Molecular Detection of Nosocomial Carbapenemase-Producing Gram-Negative Bacteria—On Near- and Distant-Patient Surfaces |
| title_short | Targeted Molecular Detection of Nosocomial Carbapenemase-Producing Gram-Negative Bacteria—On Near- and Distant-Patient Surfaces |
| title_sort | targeted molecular detection of nosocomial carbapenemase producing gram negative bacteria on near and distant patient surfaces |
| topic | molecular detection fomites carbapenemase-producing Gram-negative bacteria hygiene patient’s room |
| url | https://www.mdpi.com/2076-2607/9/6/1190 |
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